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Bioassays with diatomaceous earth formulations: Effect of species co-occurrence, size of vials and application technique

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dc.contributor.author Kavallieratos, NG en
dc.contributor.author Athanassiou, CG en
dc.contributor.author Mpassoukou, AE en
dc.contributor.author Mpakou, FD en
dc.contributor.author Tomanovic, T en
dc.contributor.author Manessioti, TB en
dc.contributor.author Papadopoulou, SC en
dc.date.accessioned 2014-06-06T06:51:39Z
dc.date.available 2014-06-06T06:51:39Z
dc.date.issued 2012 en
dc.identifier.issn 02612194 en
dc.identifier.uri http://dx.doi.org/10.1016/j.cropro.2012.06.016 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5619
dc.subject Bioassay protocol en
dc.subject Diatomaceous earth formulations en
dc.subject Laboratory bioassays en
dc.subject Rhyzopertha dominica en
dc.subject Sitophilus oryzae en
dc.subject Tribolium confusum en
dc.subject.other bioassay en
dc.subject.other coexistence en
dc.subject.other diatomite en
dc.subject.other dose-response relationship en
dc.subject.other insect en
dc.subject.other insecticide en
dc.subject.other integrated pest management en
dc.subject.other laboratory method en
dc.subject.other maize en
dc.subject.other rice en
dc.subject.other species occurrence en
dc.subject.other wheat en
dc.subject.other Bostrichidae en
dc.subject.other Coleoptera en
dc.subject.other Curculionidae en
dc.subject.other Hexapoda en
dc.subject.other Rhyzopertha dominica en
dc.subject.other Sitophilus oryzae en
dc.subject.other Tenebrionidae en
dc.subject.other Tribolium confusum en
dc.subject.other Triticum aestivum en
dc.subject.other Triticum turgidum subsp. durum en
dc.subject.other Zea mays en
dc.title Bioassays with diatomaceous earth formulations: Effect of species co-occurrence, size of vials and application technique en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.cropro.2012.06.016 en
heal.publicationDate 2012 en
heal.abstract Two series of laboratory bioassays were carried out in order to evaluate the effect of the simultaneous presence of two insect species inside the experimental vials and the effect of different sizes (50, 65, 90, 145 mm in diameter) of experimental vials on the efficacy of diatomaceous earth (DE) formulations as wheat (Triticum durum Desf.) protectants. Three DE formulations were tested; Insecto, SilicoSec and one DE enhanced with pyrethrum, PyriSec. In a third series of bioassays the influence of application technique of DE formulations in wheat and maize (Zea mays L.) on their insecticidal efficacy was also evaluated in the laboratory. Two DE formulations were tested; SilicoSec and Protect-It. The application technique was based on the duration of admixing (0.5 or 3 min) and surface treatment (without admixing) of DE formulations in grain. In all series of bioassays three species were tested; Sitophilus oryzae (L.) (Coleoptera: Curculionidae), Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae) and Tribolium confusum Jacquelin du Val (Coleoptera: Tenebrionidae). Significant differences were noted in mortality levels of the tested species among the three DE formulations and among doses for the simultaneous presence of two insect species in all combinations and the different size of vials. In contrast, no significant differences were found in the mortality levels among the adult densities of any of the insects tested. The progeny production was low for all tested species. The increase of diameter of vials used in the bioassays decreased the mortality of S. oryzae and T. confusum adults significantly whereas no significant differences were noted for R. dominica adults. The increase of dose and exposure interval reduced differences of R. dominica adult mortality in admixture durations in the case of wheat. For S. oryzae, no significant differences in adult mortality in maize were found among admixture durations while significant differences were detected in wheat. © 2012. en
heal.journalName Crop Protection en
dc.identifier.volume 42 en
dc.identifier.doi 10.1016/j.cropro.2012.06.016 en
dc.identifier.spage 170 en
dc.identifier.epage 179 en


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