Adaptation dynamics of Clostridium butyricum in high 1,3-propanediol content media

dc.contributor.author	Chatzifragkou, A	en
dc.contributor.author	Aggelis, G	en
dc.contributor.author	Gardeli, C	en
dc.contributor.author	Galiotou-Panayotou, M	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:51:36Z
dc.date.available	2014-06-06T06:51:36Z
dc.date.issued	2012	en
dc.identifier.issn	01757598	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00253-012-4003-6	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5593
dc.subject	1,3-Propanediol	en
dc.subject	Cellular lipids	en
dc.subject	Clostridium butyricum	en
dc.subject	Crude glycerol	en
dc.subject.other	1 ,3 propanediol	en
dc.subject.other	Cellular lipid	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Continuous cultivation	en
dc.subject.other	Crude glycerol	en
dc.subject.other	Exponential growth phase	en
dc.subject.other	Fatty acid composition	en
dc.subject.other	Maintenance requirement	en
dc.subject.other	Batch cell culture	en
dc.subject.other	Biochemistry	en
dc.subject.other	Bioreactors	en
dc.subject.other	Glycerol	en
dc.subject.other	Lipids	en
dc.subject.other	Unsaturated fatty acids	en
dc.subject.other	1,3 propanediol	en
dc.subject.other	glycerol	en
dc.subject.other	unsaturated fatty acid	en
dc.subject.other	1,3 propanediol	en
dc.subject.other	1,3-propanediol	en
dc.subject.other	fatty acid	en
dc.subject.other	propanediol derivative	en
dc.subject.other	adaptation	en
dc.subject.other	bioreactor	en
dc.subject.other	fatty acid	en
dc.subject.other	fermentation	en
dc.subject.other	growth rate	en
dc.subject.other	lipid	en
dc.subject.other	metabolite	en
dc.subject.other	microbial community	en
dc.subject.other	saturation	en
dc.subject.other	adaptation	en
dc.subject.other	article	en
dc.subject.other	bacterium culture	en
dc.subject.other	bioreactor	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	continuous culture	en
dc.subject.other	endospore-forming rods and cocci	en
dc.subject.other	growth rate	en
dc.subject.other	microbial growth	en
dc.subject.other	molecular dynamics	en
dc.subject.other	culture medium	en
dc.subject.other	fermentation	en
dc.subject.other	genetics	en
dc.subject.other	growth, development and aging	en
dc.subject.other	metabolism	en
dc.subject.other	microbiology	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Adaptation, Physiological	en
dc.subject.other	Bioreactors	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Culture Media	en
dc.subject.other	Fatty Acids	en
dc.subject.other	Fermentation	en
dc.subject.other	Glycerol	en
dc.subject.other	Propylene Glycols	en
dc.title	Adaptation dynamics of Clostridium butyricum in high 1,3-propanediol content media	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00253-012-4003-6	en
heal.publicationDate	2012	en
heal.abstract	Aim of the present study was to evaluate the effect of exogenous additions of 1,3-propanediol (1,3-PDO) on microbial growth and metabolites production of Clostridium butyricum VPI 1718 strain, during crude glycerol fermentation. Preliminary batch cultures in anaerobic Duran bottles revealed that early addition of 1,3-PDO caused growth cessation in rather low quantities (15 g/L), while 1,3-PDO additions during the middle exponential growth phase up to 70 g/L resulted in an almost linear decrease of the specific growth rate (μ), accompanied by reduced glycerol assimilation, with substrate consumption being used mainly for energy of maintenance requirements. During batch trials in a 3-L bioreactor, the strain proved able to withstand more than 70 g/L of both biologically produced and externally added 1,3-PDO, whereas glycerol assimilation and metabolite production were carried on at a lower rate. Adaptation of the strain in high 1,3-PDO concentration environments was validated during its continuous cultivation with pulses of 1,3-PDO in concentrations of 31 and 46 g/L, where no washout phenomena were noticed. As far as C. butyricum cellular lipids were concerned, during batch bioreactor cultivations, 1,3-PDO addition was found to favor the biosynthesis of unsaturated fatty acids. Also, fatty acid composition was studied during continuous cultures, in which additions of 1,3-PDO were performed at steady states. Lipids were globally more saturated compared to batch cultures, while by monitoring of the transitory phases, it was noticed that the gradual diol washout had an evident impact in the alteration of the fatty acid composition, by rendering them more unsaturated. © 2012 Springer-Verlag.	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.issue	6	en
dc.identifier.volume	95	en
dc.identifier.doi	10.1007/s00253-012-4003-6	en
dc.identifier.spage	1541	en
dc.identifier.epage	1552	en