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A RANKL G278R mutation causing osteopetrosis identifies a functional amino acid essential for trimer assembly in RANKL and TNF

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dc.contributor.author Douni, E en
dc.contributor.author Rinotas, V en
dc.contributor.author Makrinou, E en
dc.contributor.author Zwerina, J en
dc.contributor.author Penninger, JM en
dc.contributor.author Eliopoulos, E en
dc.contributor.author Schett, G en
dc.contributor.author Kollias, G en
dc.date.accessioned 2014-06-06T06:51:35Z
dc.date.available 2014-06-06T06:51:35Z
dc.date.issued 2012 en
dc.identifier.issn 09646906 en
dc.identifier.uri http://dx.doi.org/10.1093/hmg/ddr510 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5584
dc.subject.other arginine en
dc.subject.other glutathione transferase en
dc.subject.other glycine en
dc.subject.other monomer en
dc.subject.other osteoclast differentiation factor en
dc.subject.other receptor activator of nuclear factor kappa B en
dc.subject.other recombinant osteoclast differentiation factor en
dc.subject.other recombinant protein en
dc.subject.other tumor necrosis factor en
dc.subject.other unclassified drug en
dc.subject.other animal cell en
dc.subject.other animal experiment en
dc.subject.other animal model en
dc.subject.other animal tissue en
dc.subject.other article en
dc.subject.other autosomal recessive disorder en
dc.subject.other autosomal recessive osteopetrosis en
dc.subject.other codon en
dc.subject.other controlled study en
dc.subject.other crystal structure en
dc.subject.other ex vivo study en
dc.subject.other female en
dc.subject.other histopathology en
dc.subject.other human en
dc.subject.other human cell en
dc.subject.other hydrophobicity en
dc.subject.other in vitro study en
dc.subject.other in vivo study en
dc.subject.other loss of function mutation en
dc.subject.other male en
dc.subject.other missense mutation en
dc.subject.other mouse en
dc.subject.other nonhuman en
dc.subject.other nucleotide sequence en
dc.subject.other osteoclast en
dc.subject.other osteoclastogenesis en
dc.subject.other osteoporosis en
dc.subject.other priority journal en
dc.subject.other protein function en
dc.subject.other protein protein interaction en
dc.subject.other receptor binding en
dc.subject.other wild type en
dc.subject.other Amino Acid Substitution en
dc.subject.other Animals en
dc.subject.other Disease Models, Animal en
dc.subject.other Ethylnitrosourea en
dc.subject.other Genes, Dominant en
dc.subject.other Mice en
dc.subject.other Mutation, Missense en
dc.subject.other Osteoclasts en
dc.subject.other Osteopetrosis en
dc.subject.other Point Mutation en
dc.subject.other Protein Binding en
dc.subject.other Protein Multimerization en
dc.subject.other RANK Ligand en
dc.subject.other Receptor Activator of Nuclear Factor-kappa B en
dc.subject.other Tumor Necrosis Factor-alpha en
dc.subject.other Mus en
dc.title A RANKL G278R mutation causing osteopetrosis identifies a functional amino acid essential for trimer assembly in RANKL and TNF en
heal.type journalArticle en
heal.identifier.primary 10.1093/hmg/ddr510 en
heal.identifier.secondary ddr510 en
heal.publicationDate 2012 en
heal.abstract Receptor activator of nuclear factor-κB ligand (RANKL), a trimeric tumor necrosis factor (TNF) superfamily member, is the central mediator of osteoclast formation and bone resorption. Functional mutations in RANKL lead to human autosomal recessive osteopetrosis (ARO), whereas RANKL overexpression has been implicated in the pathogenesis of bone degenerative diseases such as osteoporosis. Following a forward genetics approach using N-ethyl-N-nitrosourea (ENU)-mediated random mutagenesis, we generated a novel mouse model of ARO caused by a new loss-of-function allele of Rankl with a glycine-to-arginine mutation at codon 278 (G278R) at the extracellular inner hydrophobic F β-strand of RANKL. Mutant mice develop severe osteopetrosis similar to Rankl-deficient mice, whereas exogenous administration of recombinant RANKL restores osteoclast formation in vivo. We show that RANKL G278R monomers fail to assemble into homotrimers, are unable to bind and activate the RANK receptor and interact with wild-type RANKL exerting a dominant-negative effect on its trimerization and function in vitro. Since G278 is highly conserved within the TNF superfamily, we identified that a similar substitution in TNF, G122R, also abrogated trimerization, binding to TNF receptor and consequently impaired TNF biological activity. Notably, SPD304, a potent small-molecule inhibitor of TNF trimerization that interacts with G122, also inhibited RANKL activity, suggesting analogous inhibitory mechanisms. Our results provide a new disease model for ARO and identify a functional amino acid in the TNF-like core domain essential for trimer formation both in RANKL and in TNF that could be considered a novel potential target for inhibiting their biological activities. © The Author 2011. Published by Oxford University Press. All rights reserved. en
heal.journalName Human Molecular Genetics en
dc.identifier.issue 4 en
dc.identifier.volume 21 en
dc.identifier.doi 10.1093/hmg/ddr510 en
dc.identifier.spage 784 en
dc.identifier.epage 798 en


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