| dc.contributor.author |
Gramberg, B |
en |
| dc.contributor.author |
Kintzios, S |
en |
| dc.contributor.author |
Schmidt, U |
en |
| dc.contributor.author |
Mewis, I |
en |
| dc.contributor.author |
Ulrichs, C |
en |
| dc.date.accessioned |
2014-06-06T06:51:33Z |
|
| dc.date.available |
2014-06-06T06:51:33Z |
|
| dc.date.issued |
2012 |
en |
| dc.identifier.issn |
09311785 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1111/j.1439-0434.2011.01867.x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5569 |
|
| dc.subject |
Antigen detection |
en |
| dc.subject |
Bioelectric recognition assay |
en |
| dc.subject |
Cherry leaf roll virus |
en |
| dc.subject |
Escherichia coli XL-1Blue MRF' |
en |
| dc.subject |
Membrane engineering |
en |
| dc.subject |
Tobacco mosaic virus |
en |
| dc.subject.other |
antibiotics |
en |
| dc.subject.other |
antigen |
en |
| dc.subject.other |
bacterium |
en |
| dc.subject.other |
bioassay |
en |
| dc.subject.other |
drug |
en |
| dc.subject.other |
genetic engineering |
en |
| dc.subject.other |
membrane |
en |
| dc.subject.other |
optimization |
en |
| dc.subject.other |
research work |
en |
| dc.subject.other |
sensor |
en |
| dc.subject.other |
virus |
en |
| dc.subject.other |
Animalia |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Cherry leaf roll virus |
en |
| dc.subject.other |
Cucumber mosaic virus |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Tobacco mosaic virus |
en |
| dc.title |
A basic approach towards the development of bioelectric bacterial biosensors for the detection of plant viruses |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1111/j.1439-0434.2011.01867.x |
en |
| heal.publicationDate |
2012 |
en |
| heal.abstract |
There is a growing need for virus sensors with improved sensitivity and dynamic range for disease diagnosis, pharmaceutical research, agriculture and homeland security. Membrane-engineered animal cells bearing antibodies against viral antigens have been previously used for biorecognition biosensors for the ultrarapid (3min), sensitive (1ng/ml) detection of plant viruses, such as the cucumber mosaic virus. We here report a new approach for the construction of cell-based sensors for virus detection, based on membrane (antibody)-engineered bacteria. The novel method was applied for the detection of tobacco mosaic virus (TMV) and cherry leaf roll virus (CLRV) using sensors containing modified Escherichia coli XL-1Blue MRF' bacteria. E. coli membranes have been engineered with electro-inserted, virus-homologous antibodies. The detection principle was based on the measurement of changes in the bacterial membrane potential as a result of virus-antibody binding. After optimization of the membrane-engineering process, the virus detection limit for TMV and CLRV with the bacteria-based biosensor system was 1pg/ml, representing a 1000-fold improvement over currently available methods. Although the novel biosensor is still in its proof-of-concept stage of development, its sensitivity and speed (assay time: 60-100s) could make it a very promising tool for high throughput, field-based virus screening. © 2011 Blackwell Verlag GmbH. |
en |
| heal.journalName |
Journal of Phytopathology |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
160 |
en |
| dc.identifier.doi |
10.1111/j.1439-0434.2011.01867.x |
en |
| dc.identifier.spage |
106 |
en |
| dc.identifier.epage |
111 |
en |