The HrpZ gene of Pseudomonas syringae pv. phaseolicola enhances resistance to rhizomania disease in transgenic Nicotiana benthamiana and sugar beet

dc.contributor.advisor	Pavli, Ourania I.	en
dc.contributor.advisor	Παυλή, Ουρανία Ι.	el
dc.contributor.author	Kelaidi, Georgia I.	en
dc.contributor.author	Tampakaki, Anastasia P.	en
dc.contributor.author	Skaracis, George N.	en
dc.contributor.author	Κελαηδή, Γεωργία Ι.	el
dc.contributor.author	Ταμπακάκη, Αναστασία Π.	el
dc.contributor.author	Σκαράκης, Γεώργιος Ν.	el
dc.date.accessioned	2014-06-06T06:51:32Z
dc.date.available	2014-06-06T06:51:32Z
dc.date.issued	2011-03-04	en
dc.identifier.issn	19326203	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0017306	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5565
dc.rights	CC0 1.0 Παγκόσμια	el
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.title	The HrpZ gene of Pseudomonas syringae pv. phaseolicola enhances resistance to rhizomania disease in transgenic Nicotiana benthamiana and sugar beet	en
heal.type	journalArticle	en
heal.keyword	Bacterial protein	en
heal.keyword	Hairpin protein	en
heal.keyword	HrpZ protein	en
heal.keyword	Pseudomonas syringae	en
heal.keyword	Virus resistance	en
heal.keyword	hrpZ gene	en
heal.keyword	Nicotiana benthamiana	en
heal.keyword	Polymerase chain reaction	en
heal.keyword	Progeny	en
heal.keyword	Root growth	en
heal.keyword	Sugar beet	en
heal.keyword	Necrosis	en
heal.keyword	Beta vulgaris	en
heal.keyword	Plant Diseases	en
heal.keyword	Plant virus	en
heal.keyword	Rhizomania Disease	en
heal.identifier.primary	10.1371/journal.pone.0017306	en
heal.identifier.secondary	e17306	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Φυτικής Παραγωγής	el
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Γεωπονικής Βιοτεχνολογίας	el
heal.publicationDate	2011-03-04	en
heal.bibliographicCitation	Pavli, Ourania I. The HrpZ gene of Pseudomonas syringae pv. phaseolicola enhances resistance to rhizomania disease in transgenic Nicotiana benthamiana and sugar beet, Plos One, vol. 6 (3), pp 1-9, PLoS, 2011	en
heal.abstract	To explore possible sources of transgenic resistance to the rhizomania-causing Beet necrotic yellow vein virus (BNYVV), Nicotiana benthamiana plants were constructed to express the harpin of Pseudomonas syringae pv. phaseolicola (HrpZPsph). The HrpZ protein was expressed as an N-terminal fusion to the PR1 signal peptide (SP/HrpZ) to direct harpin accumulation to the plant apoplast. Transgene integration was verified by mPCR in all primary transformants (T0), while immunoblot analysis confirmed that the protein HrpZPsph was produced and the signal peptide was properly processed. Neither T0 plants nor selfed progeny (T1) showed macroscopically visible necrosis or any other macroscopic phenotypes. However, plants expressing the SP/HrpZPsph showed increased vigor and grew faster in comparison with non-transgenic control plants. Transgenic resistance was assessed after challenge inoculation with BNYVV on T1 progeny by scoring of disease symptoms and by DAS-ELISA at 20 and 30 dpi. Transgenic and control lines showed significant differences in terms of the number of plants that became infected, the timing of infection and the disease symptoms displayed. Plants expressing the SP/HrpZPsph developed localized leaf necrosis in the infection area and had enhanced resistance upon challenge with BNYVV. In order to evaluate the SP/HrpZ-based resistance in the sugar beet host, A. rhizogenes-mediated root transformation was exploited as a transgene expression platform. Upon BNYVV inoculation, transgenic sugar beet hairy roots showed high level of BNYVV resistance. In contrast, the aerial non-transgenic parts of the same seedlings had virus titers that were comparable to those of the seedlings that were untransformed or transformed with wild type R1000 cells. These findings indicate that the transgenically expressed SP/HrpZ protein results in enhanced rhizomania resistance both in a model plant and sugar beet, the natural host of BNYVV. Possible molecular mechanisms underlying the enhanced resistance and plant growth phenotypes observed in SP/HrpZ transgenic plants are discussed. © 2011 Pavli et al.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLoS One	en
dc.identifier.doi	10.1371/journal.pone.0017306	en