| dc.contributor.author |
Venieraki, A |
en |
| dc.contributor.author |
Dimou, M |
en |
| dc.contributor.author |
Pergalis, P |
en |
| dc.contributor.author |
Kefalogianni, I |
en |
| dc.contributor.author |
Chatzipavlidis, I |
en |
| dc.contributor.author |
Katinakis, P |
en |
| dc.date.accessioned |
2014-06-06T06:51:29Z |
|
| dc.date.available |
2014-06-06T06:51:29Z |
|
| dc.date.issued |
2011 |
en |
| dc.identifier.issn |
00953628 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s00248-010-9747-x |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5536 |
|
| dc.subject.other |
bacterial DNA |
en |
| dc.subject.other |
indoleacetic acid derivative |
en |
| dc.subject.other |
nitrogenase reductase |
en |
| dc.subject.other |
oxidoreductase |
en |
| dc.subject.other |
RNA 16S |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Azospirillum |
en |
| dc.subject.other |
classification |
en |
| dc.subject.other |
DNA sequence |
en |
| dc.subject.other |
enzymology |
en |
| dc.subject.other |
genetic variability |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
Greece |
en |
| dc.subject.other |
growth, development and aging |
en |
| dc.subject.other |
isolation and purification |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
microbiology |
en |
| dc.subject.other |
molecular cloning |
en |
| dc.subject.other |
nitrogen fixation |
en |
| dc.subject.other |
phylogeny |
en |
| dc.subject.other |
Pseudomonas stutzeri |
en |
| dc.subject.other |
rhizosphere |
en |
| dc.subject.other |
wheat |
en |
| dc.subject.other |
Azospirillum |
en |
| dc.subject.other |
Cloning, Molecular |
en |
| dc.subject.other |
DNA, Bacterial |
en |
| dc.subject.other |
Genetic Variation |
en |
| dc.subject.other |
Greece |
en |
| dc.subject.other |
Indoleacetic Acids |
en |
| dc.subject.other |
Nitrogen Fixation |
en |
| dc.subject.other |
Oxidoreductases |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Pseudomonas stutzeri |
en |
| dc.subject.other |
Rhizosphere |
en |
| dc.subject.other |
RNA, Ribosomal, 16S |
en |
| dc.subject.other |
Sequence Analysis, DNA |
en |
| dc.subject.other |
Soil Microbiology |
en |
| dc.subject.other |
Triticum |
en |
| dc.subject.other |
Azospirillum |
en |
| dc.subject.other |
Azospirillum brasilense |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Pseudomonas stutzeri |
en |
| dc.subject.other |
Triticum aestivum |
en |
| dc.title |
The Genetic Diversity of Culturable Nitrogen-Fixing Bacteria in the Rhizosphere of Wheat |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s00248-010-9747-x |
en |
| heal.publicationDate |
2011 |
en |
| heal.abstract |
A total of 17 culturable nitrogen-fixing bacterial strains associated with the roots of wheat growing in different regions of Greece were isolated and characterized for plant-growth-promoting traits such as auxin production and phosphate solubilization. The phylogenetic position of the isolates was first assessed by the analysis of the PCR-amplified 16S rRNA gene. The comparative sequence analysis and phylogenetic analysis based on 16S rRNA gene sequences show that the isolates recovered in this study are grouped with Azospirillum brasilense, Azospirillum zeae, and Pseudomonas stutzeri. The diazotrophic nature of all isolates was confirmed by amplification of partial nifH gene sequences. The phylogenetic tree based on nifH gene sequences is consistent with 16S rRNA gene phylogeny. The isolates belonging to Azospirillum species were further characterized by examining the partial dnaK gene phylogenetic tree. Furthermore, it was demonstrated that the ipdC gene was present in all Azospirillum isolates, suggesting that auxin is mainly synthesized via the indole-3-pyruvate pathway. Although members of P. stutzeri and A. zeae are known diazotrophic bacteria, to the best of our knowledge, this is the first report of isolation and characterization of strains belonging to these bacterial genera associated with wheat. © 2010 Springer Science+Business Media, LLC. |
en |
| heal.journalName |
Microbial Ecology |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
61 |
en |
| dc.identifier.doi |
10.1007/s00248-010-9747-x |
en |
| dc.identifier.spage |
277 |
en |
| dc.identifier.epage |
285 |
en |