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Seedling vaccination by stem injecting a conidial suspension of F2, a non-pathogenic Fusarium oxysporum strain, suppresses Verticillium wilt of eggplant

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dc.contributor.author Gizi, D en
dc.contributor.author Stringlis, IA en
dc.contributor.author Tjamos, SE en
dc.contributor.author Paplomatas, EJ en
dc.date.accessioned 2014-06-06T06:51:27Z
dc.date.available 2014-06-06T06:51:27Z
dc.date.issued 2011 en
dc.identifier.issn 10499644 en
dc.identifier.uri http://dx.doi.org/10.1016/j.biocontrol.2011.06.009 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5523
dc.subject Biological control en
dc.subject Vascular wilts en
dc.subject Verticillium dahliae en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Fusarium oxysporum en
dc.subject.other Solanum melongena en
dc.subject.other Verticillium dahliae en
dc.title Seedling vaccination by stem injecting a conidial suspension of F2, a non-pathogenic Fusarium oxysporum strain, suppresses Verticillium wilt of eggplant en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.biocontrol.2011.06.009 en
heal.publicationDate 2011 en
heal.abstract Several bacterial and fungal strains have been evaluated as biocontrol agents (BCAs) against Verticillium dahliae. In these studies, the BCAs were applied as a root drenching inoculum; however, this application method may have an adverse effect on the native, beneficial for the plants, microbial community. In the present study, it was evaluated whether endophytic application by stem injecting a conidial suspension of the non pathogenic Fusarium oxysporum F2 strain, isolated from a V. dahliae suppressive compost amendment, could reduce significantly Verticillium wilt symptom development in eggplants. It was revealed that stem injection of F2 seven days before transplanting the seedlings to soil infested by V. dahliae microsclerotia resulted in reduced disease severity compared to the control treatment. To visualise F2 ramification into the plant vascular system eggplant stems were injected with an EGFP transformed F2 strain. It was shown that F2 colonises effectively the plant vascular tissues over a long period of time as it was assessed by F2 DNA levels. In parallel, qPCR analysis showed that the application of F2 reduced significantly the amount of V. dahliae DNA in the stem tissues compared to the control treatment. © 2011 Elsevier Inc. en
heal.journalName Biological Control en
dc.identifier.issue 3 en
dc.identifier.volume 58 en
dc.identifier.doi 10.1016/j.biocontrol.2011.06.009 en
dc.identifier.spage 387 en
dc.identifier.epage 392 en


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