Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process

dc.contributor.author	Chatzifragkou, A	en
dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Dietz, D	en
dc.contributor.author	Doulgeraki, AI	en
dc.contributor.author	Nychas, G-JE	en
dc.contributor.author	Zeng, A-P	en
dc.date.accessioned	2014-06-06T06:51:26Z
dc.date.available	2014-06-06T06:51:26Z
dc.date.issued	2011	en
dc.identifier.issn	01757598	en
dc.identifier.uri	http://dx.doi.org/10.1007/s00253-011-3247-x	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5509
dc.subject	1,3-Propanediol	en
dc.subject	Clostridium butyricum	en
dc.subject	Crude glycerol	en
dc.subject	Non-sterile fermentation	en
dc.subject.other	1 ,3 propanediol	en
dc.subject.other	Batch culture	en
dc.subject.other	Bioprocesses	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Continuous culture	en
dc.subject.other	Continuous operation	en
dc.subject.other	Crude glycerol	en
dc.subject.other	Culture conditions	en
dc.subject.other	Different origins	en
dc.subject.other	Dilution rate	en
dc.subject.other	Fed-batch operation	en
dc.subject.other	Fermentation process	en
dc.subject.other	Glycerol concentration	en
dc.subject.other	Gradient gel electrophoresis	en
dc.subject.other	Initial concentration	en
dc.subject.other	Kinetic data	en
dc.subject.other	Microbial communities	en
dc.subject.other	Non-sterile fermentation	en
dc.subject.other	PCR-DGGE	en
dc.subject.other	Production yield	en
dc.subject.other	Sole carbon source	en
dc.subject.other	Steady state	en
dc.subject.other	With inlets	en
dc.subject.other	Batch cell culture	en
dc.subject.other	Biodiesel	en
dc.subject.other	Clostridium	en
dc.subject.other	Cultivation	en
dc.subject.other	Electrophoresis	en
dc.subject.other	Fermentation	en
dc.subject.other	Polymerase chain reaction	en
dc.subject.other	Production	en
dc.subject.other	Glycerol	en
dc.subject.other	1,3 propanediol	en
dc.subject.other	biodiesel	en
dc.subject.other	glycerol	en
dc.subject.other	alcohol	en
dc.subject.other	bacterium	en
dc.subject.other	biofuel	en
dc.subject.other	biotechnology	en
dc.subject.other	chemostat	en
dc.subject.other	concentration (composition)	en
dc.subject.other	electrokinesis	en
dc.subject.other	fermentation	en
dc.subject.other	microbial activity	en
dc.subject.other	microbial community	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	reaction kinetics	en
dc.subject.other	alcohol production	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial strain	en
dc.subject.other	bacterium culture	en
dc.subject.other	bioprocess	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	controlled study	en
dc.subject.other	denaturing gradient gel electrophoresis	en
dc.subject.other	fed batch fermentation	en
dc.subject.other	microbial community	en
dc.subject.other	nonhuman	en
dc.subject.other	polymerase chain reaction	en
dc.subject.other	Biofuels	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Culture Media	en
dc.subject.other	Fermentation	en
dc.subject.other	Glycerol	en
dc.subject.other	Industrial Microbiology	en
dc.subject.other	Propylene Glycols	en
dc.subject.other	Sterilization	en
dc.subject.other	Clostridium butyricum	en
dc.title	Production of 1,3-propanediol by Clostridium butyricum growing on biodiesel-derived crude glycerol through a non-sterilized fermentation process	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s00253-011-3247-x	en
heal.publicationDate	2011	en
heal.abstract	The aim of the present study was to investigate the production of 1,3-propanediol (PDO) under non-sterile fermentation conditions by employing the strain Clostridium butyricum VPI 1718. A series of batch cultures were performed by utilizing biodiesel-derived crude glycerol feedstocks of different origins as the sole carbon source, in various initial concentrations. The strain presented similarities in terms of PDO production when cultivated on crude glycerol of various origins, with final concentrations ranging between 11.1 and 11.5 g/L. Moreover, PDO fermentation was successfully concluded regardless of the initial crude glycerol concentration imposed (from 20 to 80 g/L), accompanied by sufficient PDO production yields (0.52-0.55 g per gram of glycerol consumed). During fed-batch operation under non-sterile culture conditions, 67.9 g/L of PDO were finally produced, with a yield of 0.55 g/g. Additionally, the sustainability of the bioprocess during a continuous operation was tested; indeed, the system was able to run at steady state for 16 days, during which PDO effluent level was 13.9 g/L. Furthermore, possible existence of a microbial community inside the chemostat was evaluated by operating a polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis, and DGGE results revealed the presence of only one band corresponding to that of C. butyricum VPI 1718. Finally, non-sterile continuous cultures were carried out at different dilution rates (D), with inlet glycerol concentration at 80 g/L. Maximum PDO production was achieved at low D values (0.02 h -1) corresponding to 30.1 g/L, while the elaboration of kinetic data from continuous cultures revealed the stability of the bioprocess proposed, with global PDO production yield corresponding to 0.52 g/g. © 2011 Springer-Verlag.	en
heal.journalName	Applied Microbiology and Biotechnology	en
dc.identifier.issue	1	en
dc.identifier.volume	91	en
dc.identifier.doi	10.1007/s00253-011-3247-x	en
dc.identifier.spage	101	en
dc.identifier.epage	112	en