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Potential of a simple HPLC-based approach for the identification of the spoilage status of minced beef stored at various temperatures and packaging systems

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dc.contributor.author Argyri, AA en
dc.contributor.author Doulgeraki, AI en
dc.contributor.author Blana, VA en
dc.contributor.author Panagou, EZ en
dc.contributor.author Nychas, GJE en
dc.date.accessioned 2014-06-06T06:51:25Z
dc.date.available 2014-06-06T06:51:25Z
dc.date.issued 2011 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/j.ijfoodmicro.2011.07.010 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5505
dc.subject Active packaging en
dc.subject Bioinformatics en
dc.subject Essential oil en
dc.subject HPLC en
dc.subject Meat spoilage en
dc.subject Metabolomics en
dc.subject.other article en
dc.subject.other Brochothrix thermosphacta en
dc.subject.other controlled study en
dc.subject.other discriminant analysis en
dc.subject.other Enterobacteriaceae en
dc.subject.other food packaging en
dc.subject.other food quality en
dc.subject.other food spoilage en
dc.subject.other food storage en
dc.subject.other high performance liquid chromatography en
dc.subject.other lactic acid bacterium en
dc.subject.other meat en
dc.subject.other modified atmosphere packaging en
dc.subject.other pH measurement en
dc.subject.other principal component analysis en
dc.subject.other Pseudomonas en
dc.subject.other Animals en
dc.subject.other Bacteria en
dc.subject.other Cattle en
dc.subject.other Chromatography, High Pressure Liquid en
dc.subject.other Colony Count, Microbial en
dc.subject.other Food Microbiology en
dc.subject.other Food Packaging en
dc.subject.other Food Preservation en
dc.subject.other Humans en
dc.subject.other Hydrogen-Ion Concentration en
dc.subject.other Meat en
dc.subject.other Oils, Volatile en
dc.subject.other Origanum en
dc.subject.other Plant Extracts en
dc.subject.other Temperature en
dc.subject.other Yeasts en
dc.subject.other Brochothrix thermosphacta en
dc.subject.other Enterobacteriaceae en
dc.subject.other Origanum en
dc.subject.other Pseudomonas en
dc.title Potential of a simple HPLC-based approach for the identification of the spoilage status of minced beef stored at various temperatures and packaging systems en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.ijfoodmicro.2011.07.010 en
heal.publicationDate 2011 en
heal.abstract The shelf life of minced beef stored (i) aerobically, (ii) under modified atmosphere packaging (MAP), and (iii) under MAP with oregano essential oil (MAP/OEO) at 0, 5, 10, and 15 °C was investigated. The microbial association of meat and the temporal biochemical changes were monitored. Microbiological analyses, including total viable counts (TVC), Pseudomonas spp., Brochothrix thermosphacta, lactic acid bacteria, Enterobacteriaceae, and yeasts/moulds, were undertaken, in parallel with sensory assessment, pH measurement and HPLC analysis of the organic acid profiles. Spectral data collected by HPLC were subjected to statistical analysis, including principal component analysis (PCA) and factorial discriminant analysis (FDA). This revealed qualitative discrimination of the samples based on their spoilage status. Partial least squares regression (PLS-R) was used to evaluate quantitative predictions of TVC, Pseudomonas spp., Br. thermosphacta, lactic acid bacteria, Enterobacteriaceae, and yeasts/moulds. Overall, the HPLC analysis of organic acids, was found to be a potential method to evaluate the spoilage and microbial status of a meat sample regardless of the storage conditions. This could be a very useful tool for monitoring the quality of meat batches during transportation and storage in the meat food chain. © 2011 Elsevier B.V. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.issue 1 en
dc.identifier.volume 150 en
dc.identifier.doi 10.1016/j.ijfoodmicro.2011.07.010 en
dc.identifier.spage 25 en
dc.identifier.epage 33 en


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