Potential for bioremediation of agro-industrial effluents with high loads of pesticides by selected fungi

dc.contributor.author	Karas, PA	en
dc.contributor.author	Perruchon, C	en
dc.contributor.author	Exarhou, K	en
dc.contributor.author	Ehaliotis, C	en
dc.contributor.author	Karpouzas, DG	en
dc.date.accessioned	2014-06-06T06:51:25Z
dc.date.available	2014-06-06T06:51:25Z
dc.date.issued	2011	en
dc.identifier.issn	09239820	en
dc.identifier.uri	http://dx.doi.org/10.1007/s10532-010-9389-1	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5504
dc.subject	Aspergillus niger	en
dc.subject	Biodegradation	en
dc.subject	Fruit packaging industrial effluents	en
dc.subject	Fungicides	en
dc.subject	White rot fungi	en
dc.subject.other	Agro-industrial effluents	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Chlorpyrifos	en
dc.subject.other	Environmental discharge	en
dc.subject.other	Fruit packaging	en
dc.subject.other	Fruit packaging industrial effluents	en
dc.subject.other	High load	en
dc.subject.other	Imazalil	en
dc.subject.other	Lac activity	en
dc.subject.other	Laccases	en
dc.subject.other	Maximum Efficiency	en
dc.subject.other	Pesticide degradation	en
dc.subject.other	Phanerochaete chrysosporium	en
dc.subject.other	Pleurotus ostreatus	en
dc.subject.other	Scaling-up	en
dc.subject.other	SEM	en
dc.subject.other	Soil extracts	en
dc.subject.other	T. versicolor	en
dc.subject.other	Thiabendazole	en
dc.subject.other	Trametes versicolor	en
dc.subject.other	White rot fungi	en
dc.subject.other	Agricultural chemicals	en
dc.subject.other	Biodegradation	en
dc.subject.other	Biological water treatment	en
dc.subject.other	Bioremediation	en
dc.subject.other	Biotechnology	en
dc.subject.other	Catalysts	en
dc.subject.other	Effluents	en
dc.subject.other	Enzymes	en
dc.subject.other	Fruits	en
dc.subject.other	Fungi	en
dc.subject.other	Fungicides	en
dc.subject.other	Industry	en
dc.subject.other	Microbiology	en
dc.subject.other	Packaging	en
dc.subject.other	Phenols	en
dc.subject.other	Pollution	en
dc.subject.other	Sewage	en
dc.subject.other	Spoilage	en
dc.subject.other	Wastewater	en
dc.subject.other	Wastewater treatment	en
dc.subject.other	Degradation	en
dc.subject.other	fungal protein	en
dc.subject.other	laccase	en
dc.subject.other	peroxidase	en
dc.subject.other	pesticide	en
dc.subject.other	biodegradation	en
dc.subject.other	bioremediation	en
dc.subject.other	enzyme activity	en
dc.subject.other	fungicide	en
dc.subject.other	fungus	en
dc.subject.other	straw	en
dc.subject.other	wastewater	en
dc.subject.other	agriculture	en
dc.subject.other	article	en
dc.subject.other	Basidiomycetes	en
dc.subject.other	bioremediation	en
dc.subject.other	enzymology	en
dc.subject.other	genetics	en
dc.subject.other	metabolism	en
dc.subject.other	microbiology	en
dc.subject.other	sewage	en
dc.subject.other	water pollutant	en
dc.subject.other	Agriculture	en
dc.subject.other	Basidiomycota	en
dc.subject.other	Biodegradation, Environmental	en
dc.subject.other	Fungal Proteins	en
dc.subject.other	Laccase	en
dc.subject.other	Peroxidase	en
dc.subject.other	Pesticides	en
dc.subject.other	Sewage	en
dc.subject.other	Water Pollutants, Chemical	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Fungi	en
dc.subject.other	Phanerochaete	en
dc.subject.other	Phanerochaete chrysosporium	en
dc.subject.other	Pleurotus ostreatus	en
dc.subject.other	Trametes versicolor	en
dc.title	Potential for bioremediation of agro-industrial effluents with high loads of pesticides by selected fungi	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s10532-010-9389-1	en
heal.publicationDate	2011	en
heal.abstract	Wastewaters from the fruit packaging industry contain a high pesticide load and require treatment before their environmental discharge. We provide first evidence for the potential bioremediation of these wastewaters. Three white rot fungi (WRF) (Phanerochaete chrysosporium, Trametes versicolor, Pleurotus ostreatus) and an Aspergillus niger strain were tested in straw extract medium (StEM) and soil extract medium (SEM) for degrading the pesticides thiabendazole (TBZ), imazalil (IMZ), thiophanate methyl (TM), ortho-phenylphenol (OPP), diphenylamine (DPA) and chlorpyrifos (CHL). Peroxidase (LiP, MnP) and laccase (Lac) activity was also determined to investigate their involvement in pesticide degradation. T. versicolor and P. ostreatus were the most efficient degraders and degraded all pesticides (10 mg l-1) except TBZ, with maximum efficiency in StEM. The phenolic pesticides OPP and DPA were rapidly degraded by these two fungi with a concurrent increase in MnP and Lac activity. In contrast, these enzymes were not associated with the degradation of CHL, IMZ and TM implying the involvement of other enzymes. T. versicolor degraded spillage-level pesticide concentrations (50 mg l-1) either fully (DPA, OPP) or partially (TBZ, IMZ). The fungus was also able to rapidly degrade a mixture of TM/DPA (50 mg l-1), whereas it failed to degrade IMZ and TBZ when supplied in a mixture with OPP. Overall, T. versicolor and P. ostreatus showed great potential for the bioremediation of wastewaters from the fruit packaging industry. However, degradation of TBZ should be also achieved before further scaling up. © 2010 Springer Science+Business Media B.V.	en
heal.journalName	Biodegradation	en
dc.identifier.issue	1	en
dc.identifier.volume	22	en
dc.identifier.doi	10.1007/s10532-010-9389-1	en
dc.identifier.spage	215	en
dc.identifier.epage	228	en