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Polar tube protein gene diversity among Nosema ceranae strains derived from a Greek honey bee health study

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dc.contributor.author Hatjina, F en
dc.contributor.author Tsoktouridis, G en
dc.contributor.author Bouga, M en
dc.contributor.author Charistos, L en
dc.contributor.author Evangelou, V en
dc.contributor.author Avtzis, D en
dc.contributor.author Meeus, I en
dc.contributor.author Brunain, M en
dc.contributor.author Smagghe, G en
dc.contributor.author De Graaf, DC en
dc.date.accessioned 2014-06-06T06:51:25Z
dc.date.available 2014-06-06T06:51:25Z
dc.date.issued 2011 en
dc.identifier.issn 00222011 en
dc.identifier.uri http://dx.doi.org/10.1016/j.jip.2011.07.003 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5501
dc.subject Apis mellifera en
dc.subject Genotyping en
dc.subject Honey bee en
dc.subject Nosema ceranae en
dc.subject Pathogen en
dc.subject Polar tube protein en
dc.subject Virus en
dc.subject.other fungal DNA en
dc.subject.other fungal protein en
dc.subject.other animal en
dc.subject.other animal disease en
dc.subject.other article en
dc.subject.other bee en
dc.subject.other environmental monitoring en
dc.subject.other genetic polymorphism en
dc.subject.other genetic variability en
dc.subject.other genetics en
dc.subject.other Greece en
dc.subject.other host pathogen interaction en
dc.subject.other microbiology en
dc.subject.other mycosis en
dc.subject.other Nosema en
dc.subject.other species difference en
dc.subject.other Animals en
dc.subject.other Bees en
dc.subject.other DNA, Fungal en
dc.subject.other Environmental Monitoring en
dc.subject.other Fungal Proteins en
dc.subject.other Genetic Variation en
dc.subject.other Greece en
dc.subject.other Host-Pathogen Interactions en
dc.subject.other Mycoses en
dc.subject.other Nosema en
dc.subject.other Polymorphism, Genetic en
dc.subject.other Species Specificity en
dc.subject.other Apis mellifera en
dc.subject.other Apoidea en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Chronic bee paralysis virus en
dc.subject.other Deformed wing virus en
dc.subject.other Encephalitozoon en
dc.subject.other Encephalitozoon cuniculi en
dc.subject.other Encephalitozoon hellem en
dc.subject.other Melissococcus plutonius en
dc.subject.other Microsporidia en
dc.subject.other Nosema en
dc.subject.other Nosema apis en
dc.subject.other Nosema ceranae en
dc.subject.other Paenibacillus en
dc.subject.other Paenibacillus larvae en
dc.title Polar tube protein gene diversity among Nosema ceranae strains derived from a Greek honey bee health study en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.jip.2011.07.003 en
heal.publicationDate 2011 en
heal.abstract Honey bee samples from 54 apiaries originating from 37 geographic locations of Greece were screened for Nosema apis and Nosema ceranae. Furthermore 15 samples coming from 12 geographic locations were screened also for Paenibacillus larvae and Melissococcus plutonius and seven honey bee virus species, for the first time on a nation-wide level. There was a tendency in finding proportionally higher spore counts in samples from apiaries that suffered important colony losses. P. larvae bacteria were identified in two samples and each of the tested bee viruses could be detected in at least one of the examined samples, with IAPV, CBPV and SBV being the least abundant and BQCV and DWV being the most abundant. In the study we focused on polymorphism of a N. ceranae gene encoding a polar tube protein (PTP) as similar genes were proven to be highly polymorphic in the microsporidian parasites Encephalitozoon cuniculi and Encephalitozoon hellem. The polymorphism observed in the PTP gene sequences from a single sample (bee hive) was unexpected and can thus be considered to be a major obstacle for genotyping. © 2011 Elsevier Inc. en
heal.journalName Journal of Invertebrate Pathology en
dc.identifier.issue 2 en
dc.identifier.volume 108 en
dc.identifier.doi 10.1016/j.jip.2011.07.003 en
dc.identifier.spage 131 en
dc.identifier.epage 134 en


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