| dc.contributor.author |
Bountouri, M |
en |
| dc.contributor.author |
Fragkiadaki, E |
en |
| dc.contributor.author |
Ntafis, V |
en |
| dc.contributor.author |
Kanellos, T |
en |
| dc.contributor.author |
Xylouri, E |
en |
| dc.date.accessioned |
2014-06-06T06:51:25Z |
|
| dc.date.available |
2014-06-06T06:51:25Z |
|
| dc.date.issued |
2011 |
en |
| dc.identifier.issn |
1743422X |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1186/1743-422X-8-350 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5497 |
|
| dc.subject.other |
virus RNA |
en |
| dc.subject.other |
hemagglutinin |
en |
| dc.subject.other |
sialidase |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cell line |
en |
| dc.subject.other |
cluster analysis |
en |
| dc.subject.other |
DNA sequence |
en |
| dc.subject.other |
dog |
en |
| dc.subject.other |
genetic reassortment |
en |
| dc.subject.other |
genetics |
en |
| dc.subject.other |
Greece |
en |
| dc.subject.other |
hemagglutination inhibition test |
en |
| dc.subject.other |
horse |
en |
| dc.subject.other |
horse disease |
en |
| dc.subject.other |
Influenza virus A H3N8 |
en |
| dc.subject.other |
isolation and purification |
en |
| dc.subject.other |
molecular genetics |
en |
| dc.subject.other |
nose mucosa |
en |
| dc.subject.other |
orthomyxovirus infection |
en |
| dc.subject.other |
phylogeny |
en |
| dc.subject.other |
reverse transcription polymerase chain reaction |
en |
| dc.subject.other |
virology |
en |
| dc.subject.other |
virus culture |
en |
| dc.subject.other |
acute respiratory tract disease |
en |
| dc.subject.other |
amino acid sequence |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
animal experiment |
en |
| dc.subject.other |
cell strain |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
equine influenza |
en |
| dc.subject.other |
hemagglutination |
en |
| dc.subject.other |
molecular dynamics |
en |
| dc.subject.other |
molecular evolution |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
nose smear |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
virus isolation |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Cell Line |
en |
| dc.subject.other |
Cluster Analysis |
en |
| dc.subject.other |
Dogs |
en |
| dc.subject.other |
Greece |
en |
| dc.subject.other |
Hemagglutination Inhibition Tests |
en |
| dc.subject.other |
Horse Diseases |
en |
| dc.subject.other |
Horses |
en |
| dc.subject.other |
Influenza A Virus, H3N8 Subtype |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Nasal Mucosa |
en |
| dc.subject.other |
Orthomyxoviridae Infections |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Reassortant Viruses |
en |
| dc.subject.other |
Reverse Transcriptase Polymerase Chain Reaction |
en |
| dc.subject.other |
RNA, Viral |
en |
| dc.subject.other |
Sequence Analysis, DNA |
en |
| dc.subject.other |
Virus Cultivation |
en |
| dc.subject.other |
Equidae |
en |
| dc.subject.other |
Equine influenza virus |
en |
| dc.subject.other |
Orthomyxoviridae |
en |
| dc.title |
Phylogenetic and molecular characterization of equine H3N8 influenza viruses from Greece (2003 and 2007): Evidence for reassortment between evolutionary lineages |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1186/1743-422X-8-350 |
en |
| heal.identifier.secondary |
350 |
en |
| heal.publicationDate |
2011 |
en |
| heal.abstract |
Background: For first time in Greece equine influenza virus infection was confirmed, by isolation and molecular analysis, as the cause of clinical respiratory disease among unvaccinated horses during 2003 and 2007 outbreaks. Methods. Equine influenza virus (EIV) H3N8 was isolated in MDCK cells from 30 nasal swabs from horses with acute respiratory disease, which were tested positive by Directigen Flu A. Isolation was confirmed by haemagglutination assay and RT-PCR assay of the M, HA and NA gene. Results: HA sequences of the Greek isolates appeared to be more closely related to viruses isolated in early 1990s in Europe. These results suggested that viruses with fewer changes than those on the main evolutionary lineage may continue to circulate. On the other hand, analysis of deduced NA amino acid sequences were more closely related to viruses isolated in outbreaks in Europe and Asia during 2003-2007. Phylogenetic analysis characterized the Greek isolates as a member of the Eurasian lineage by the haemagglutinin (HA) protein alignment, but appeared to be a member of the Florida sublineage clade 2 by the neuraminidase (NA) protein sequence suggesting that reassortment might be a possible explanation. Conclusion: Our findings suggest that the Greek strains represent an example of ""frozen evolution"" and probably reassortment between genetically distinct co-circulated strains. Therefore expanding current equine influenza surveillance efforts is a necessity. © 2011 Bountouri et al; licensee BioMed Central Ltd. |
en |
| heal.journalName |
Virology Journal |
en |
| dc.identifier.volume |
8 |
en |
| dc.identifier.doi |
10.1186/1743-422X-8-350 |
en |