| dc.contributor.author |
Fasseas, MK |
en |
| dc.contributor.author |
Tsikou, D |
en |
| dc.contributor.author |
Flemetakis, E |
en |
| dc.contributor.author |
Katinakis, P |
en |
| dc.date.accessioned |
2014-06-06T06:51:23Z |
|
| dc.date.available |
2014-06-06T06:51:23Z |
|
| dc.date.issued |
2011 |
en |
| dc.identifier.issn |
03014851 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1007/s11033-010-0292-y |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5483 |
|
| dc.subject |
α Class |
en |
| dc.subject |
C. elegans |
en |
| dc.subject |
Carbonic anhydrase |
en |
| dc.subject |
Nematode |
en |
| dc.subject |
Stress |
en |
| dc.subject.other |
carbon dioxide |
en |
| dc.subject.other |
carbonate dehydratase |
en |
| dc.subject.other |
carbonate dehydratase III |
en |
| dc.subject.other |
carbonate dehydratase IVA |
en |
| dc.subject.other |
carbonate dehydratase V |
en |
| dc.subject.other |
oxygen |
en |
| dc.subject.other |
unclassified drug |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
Caenorhabditis elegans |
en |
| dc.subject.other |
cah 1 gene |
en |
| dc.subject.other |
cah 2 gene |
en |
| dc.subject.other |
cah 3 gene |
en |
| dc.subject.other |
cah 4 gene |
en |
| dc.subject.other |
cah 5 gene |
en |
| dc.subject.other |
cah 6 gene |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
environmental stress |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
gene |
en |
| dc.subject.other |
gene expression |
en |
| dc.subject.other |
genome analysis |
en |
| dc.subject.other |
lifespan |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
pH |
en |
| dc.subject.other |
Saccharomyces cerevisiae |
en |
| dc.subject.other |
sequence analysis |
en |
| dc.subject.other |
survival rate |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Caenorhabditis elegans |
en |
| dc.subject.other |
Caenorhabditis elegans Proteins |
en |
| dc.subject.other |
Carbonic Anhydrases |
en |
| dc.subject.other |
Electrophoresis, Polyacrylamide Gel |
en |
| dc.subject.other |
Enzyme Assays |
en |
| dc.subject.other |
Gene Expression Regulation, Developmental |
en |
| dc.subject.other |
Gene Expression Regulation, Enzymologic |
en |
| dc.subject.other |
Genetic Complementation Test |
en |
| dc.subject.other |
Longevity |
en |
| dc.subject.other |
RNA Interference |
en |
| dc.subject.other |
RNA, Messenger |
en |
| dc.subject.other |
Saccharomyces cerevisiae |
en |
| dc.subject.other |
Sequence Alignment |
en |
| dc.subject.other |
Sequence Analysis, Protein |
en |
| dc.subject.other |
Caenorhabditis elegans |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Saccharomyces cerevisiae |
en |
| dc.title |
Molecular and biochemical analysis of the α class carbonic anhydrases in Caenorhabditis elegans |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1007/s11033-010-0292-y |
en |
| heal.publicationDate |
2011 |
en |
| heal.abstract |
In this study, in silico analysis of the Caenorhabditis elegans genome revealed six genes (cah-1, cah-2, cah-3, cah-4, cah-5, and cah-6) possibly encoding α class CAs (carbonic anhydrase). Real-time RT-PCR analysis revealed the temporal expression pattern of each gene, as well as changes in expression levels under different atmospheric conditions (stress). Cah-3 and cah-4 showed the highest levels of transcript accumulation, while most genes responded to the stress conditions. Yeast complementation showed that cah-3 was able to complement the function of Saccharomyces cerevisiae CA (NCE103) in vivo. Recombinant CAH-3, CAH-4a and CAH-5 enzymes, expressed in Escherichia coli were used for in vitro measurement of CA activity. However, in vitro activity was only detectable for CAH-4a. RNAi by feeding was performed on wild-type C. elegans for all genes. The worms were examined for a visible phenotype under normal and stress conditions (pH, CO2/O2). Silencing cah-3 and cah-4 may reduce the life-span of the worms (at 22°C). © 2010 Springer Science+Business Media B.V. |
en |
| heal.journalName |
Molecular Biology Reports |
en |
| dc.identifier.issue |
3 |
en |
| dc.identifier.volume |
38 |
en |
| dc.identifier.doi |
10.1007/s11033-010-0292-y |
en |
| dc.identifier.spage |
1777 |
en |
| dc.identifier.epage |
1785 |
en |