Lipid synthesized by micro-algae grown in laboratory- and industrial-scale bioreactors

dc.contributor.author	Makri, A	en
dc.contributor.author	Bellou, S	en
dc.contributor.author	Birkou, M	en
dc.contributor.author	Papatrehas, K	en
dc.contributor.author	Dolapsakis, NP	en
dc.contributor.author	Bokas, D	en
dc.contributor.author	Papanikolaou, S	en
dc.contributor.author	Aggelis, G	en
dc.date.accessioned	2014-06-06T06:51:22Z
dc.date.available	2014-06-06T06:51:22Z
dc.date.issued	2011	en
dc.identifier.issn	16180240	en
dc.identifier.uri	http://dx.doi.org/10.1002/elsc.201000086	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5474
dc.subject	Large-scale application	en
dc.subject	Lipids	en
dc.subject	Micro-algae	en
dc.subject.other	Aquatic environments	en
dc.subject.other	Docosahexaenoic acid	en
dc.subject.other	Dry biomass	en
dc.subject.other	Eicosapentaenoic acid	en
dc.subject.other	Fatty acid methyl ester	en
dc.subject.other	Glycolipids	en
dc.subject.other	High concentration	en
dc.subject.other	Isolated strains	en
dc.subject.other	Large-scale applications	en
dc.subject.other	Lipid fractions	en
dc.subject.other	Micro-algae	en
dc.subject.other	Neutral lipid	en
dc.subject.other	Pharmaceutical industry	en
dc.subject.other	Sphingolipids	en
dc.subject.other	Algae	en
dc.subject.other	Bioreactors	en
dc.subject.other	Esters	en
dc.subject.other	Fatty acids	en
dc.subject.other	Industry	en
dc.subject.other	Phospholipids	en
dc.subject.other	Polyunsaturated fatty acids	en
dc.subject.other	docosahexaenoic acid	en
dc.subject.other	fatty acid ester	en
dc.subject.other	glycolipid	en
dc.subject.other	icosapentaenoic acid	en
dc.subject.other	lipid	en
dc.subject.other	octadecatetraenoic acid	en
dc.subject.other	omega 3 fatty acid	en
dc.subject.other	phospholipid	en
dc.subject.other	sphingolipid	en
dc.subject.other	unclassified drug	en
dc.subject.other	aquaculture industry	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	biotechnology	en
dc.subject.other	carboxylic acid	en
dc.subject.other	ester	en
dc.subject.other	food industry	en
dc.subject.other	laboratory method	en
dc.subject.other	lipid	en
dc.subject.other	marine technology	en
dc.subject.other	microalga	en
dc.subject.other	pharmaceutical industry	en
dc.subject.other	alga	en
dc.subject.other	algal growth	en
dc.subject.other	Amphidinium	en
dc.subject.other	aquatic environment	en
dc.subject.other	article	en
dc.subject.other	Asterionella	en
dc.subject.other	biomass	en
dc.subject.other	bioreactor	en
dc.subject.other	concentration (parameters)	en
dc.subject.other	laboratory	en
dc.subject.other	lipogenesis	en
dc.subject.other	microalga	en
dc.subject.other	Nannochloropsis oculata	en
dc.subject.other	nonhuman	en
dc.subject.other	Prorocentrum minimum	en
dc.subject.other	Prorocentrum triestinum	en
dc.subject.other	Prymnesium	en
dc.subject.other	Tetraselmis	en
dc.subject.other	Greece	en
dc.subject.other	algae	en
dc.subject.other	Amphidinium	en
dc.subject.other	Asterionella	en
dc.subject.other	Nannochloropsis oculata	en
dc.subject.other	Prorocentrum minimum	en
dc.subject.other	Prorocentrum triestinum	en
dc.subject.other	Prymnesium parvum	en
dc.subject.other	Tetraselmis	en
dc.title	Lipid synthesized by micro-algae grown in laboratory- and industrial-scale bioreactors	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/elsc.201000086	en
heal.publicationDate	2011	en
heal.abstract	Tetraselmis sp. and Nannochloropsis oculata, cultivated in industrial-scale bioreactors, produced 2.33 and 2.44%w/w lipid (calculated as the sum of fatty acid methyl esters) in dry biomass, respectively. These lipids contained higher amounts of neutral lipids and glycolipids plus sphingolipids, than phospholipids. Lipids of Tetraselmis sp. were characterized by the presence of eicosapentaenoic acid (that was located mainly in phospholipids), and octadecatetraenoic acid (that was equally distributed among lipid fractions), while these fatty acids were completely absent in N. oculata lipids. Additionally, lipids produced by 16 newly isolated strains from Greek aquatic environments (cultivated in flask reactors) were studied. The highest percentage of lipids was found in Prorocentrum triestinum (3.69% w/w) while the lowest in Prymnesium parvum (0.47% w/w). Several strains produced lipids rich in eicosapentaenoic and docosahexaenoic acids. For instance, docosahexaenoic acid was found in high percentages in lipids of Amphidinium sp. S1, P. parvum, Prorocentrum minimum and P. triestinum, while lipids produced by Asterionella sp. (?) S2 contained eicosapentaenoic acid in high concentration. These lipids, containing ω-3-long-chain polyunsaturated fatty acids, have important applications in the food and pharmaceutical industries and in aquaculture. © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.	en
heal.journalName	Engineering in Life Sciences	en
dc.identifier.issue	1	en
dc.identifier.volume	11	en
dc.identifier.doi	10.1002/elsc.201000086	en
dc.identifier.spage	52	en
dc.identifier.epage	58	en