| dc.contributor.author |
Ntafis, V |
en |
| dc.contributor.author |
Mari, V |
en |
| dc.contributor.author |
Decaro, N |
en |
| dc.contributor.author |
Papanastassopoulou, M |
en |
| dc.contributor.author |
Papaioannou, N |
en |
| dc.contributor.author |
Mpatziou, R |
en |
| dc.contributor.author |
Buonavoglia, C |
en |
| dc.contributor.author |
Xylouri, E |
en |
| dc.date.accessioned |
2014-06-06T06:51:22Z |
|
| dc.date.available |
2014-06-06T06:51:22Z |
|
| dc.date.issued |
2011 |
en |
| dc.identifier.issn |
03781135 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.vetmic.2011.03.008 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5472 |
|
| dc.subject |
CCoV |
en |
| dc.subject |
Distribution |
en |
| dc.subject |
Dog |
en |
| dc.subject |
Organs |
en |
| dc.subject |
Recombination |
en |
| dc.subject |
TGEV-like |
en |
| dc.subject.other |
virus RNA |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
animal experiment |
en |
| dc.subject.other |
animal tissue |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
canine coronavirus type i |
en |
| dc.subject.other |
canine coronavirus type ii |
en |
| dc.subject.other |
canine coronavirus type iia |
en |
| dc.subject.other |
canine coronavirus type iib |
en |
| dc.subject.other |
canine parvovirus type 2 |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
Coronavirus |
en |
| dc.subject.other |
dog |
en |
| dc.subject.other |
feces |
en |
| dc.subject.other |
mixed infection |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
nucleotide sequence |
en |
| dc.subject.other |
organ |
en |
| dc.subject.other |
Parvovirus |
en |
| dc.subject.other |
phylogeny |
en |
| dc.subject.other |
puppy |
en |
| dc.subject.other |
real time polymerase chain reaction |
en |
| dc.subject.other |
sequence analysis |
en |
| dc.subject.other |
tissue distribution |
en |
| dc.subject.other |
Transmissible gastroenteritis virus |
en |
| dc.subject.other |
virus characterization |
en |
| dc.subject.other |
virus detection |
en |
| dc.subject.other |
virus isolation |
en |
| dc.subject.other |
virus load |
en |
| dc.subject.other |
virus strain |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Coronavirus Infections |
en |
| dc.subject.other |
Coronavirus, Canine |
en |
| dc.subject.other |
Dog Diseases |
en |
| dc.subject.other |
Dogs |
en |
| dc.subject.other |
Enteritis |
en |
| dc.subject.other |
Feces |
en |
| dc.subject.other |
Genotype |
en |
| dc.subject.other |
Parvovirus, Canine |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Reverse Transcriptase Polymerase Chain Reaction |
en |
| dc.subject.other |
RNA, Viral |
en |
| dc.subject.other |
Sequence Analysis, RNA |
en |
| dc.subject.other |
Canine coronavirus |
en |
| dc.subject.other |
Canine parvovirus 2 |
en |
| dc.subject.other |
Canis familiaris |
en |
| dc.subject.other |
RNA viruses |
en |
| dc.subject.other |
Transmissible gastroenteritis virus |
en |
| dc.title |
Isolation, tissue distribution and molecular characterization of two recombinant canine coronavirus strains |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.vetmic.2011.03.008 |
en |
| heal.publicationDate |
2011 |
en |
| heal.abstract |
Canine coronavirus (CCoV) is an enveloped RNA virus, responsible for gastrointestinal infection in dogs. To date, two different CCoV genotypes have been recognized, CCoV type I and CCoV type II. Recently, CCoV type II strains of potential recombinant origin with transmissible gastroenteritis virus (TGEV) were detected and characterized as a new subtype (CCoV-IIb) of canine coronavirus, in order to be differentiated from the "" classical"" CCoV type II strains (CCoV-IIa). In the present study, two CCoV-IIb strains were detected in the faeces and internal organs of two puppies, which died after presenting gastrointestinal symptoms. Mixed infection of both subtypes (CCoV-IIa/IIb) was detected in the faeces, while only CCoV-IIb was detected in the organs. Puppies were also infected by canine parvovirus type 2 (CPV-2). Both CCoV-IIb strains were isolated on cell cultures and subjected to sequence analysis and phylogeny. By means of RT-PCR and real time RT-PCR assays, tissue distribution and quantitation of viral loads took place. These cases represent the first description of tissue distribution and quantitation of CCoV-IIb strains, detected in the organs. The detection of CCoV-IIa strains, which is restricted to the faeces, suggests that CCoV-IIb strains may have an advantage in disseminating throughout a dog with CPV-2 coinfection, in contrast to common enteric CCoV-IIa strains. © 2011 Elsevier B.V. |
en |
| heal.journalName |
Veterinary Microbiology |
en |
| dc.identifier.issue |
3-4 |
en |
| dc.identifier.volume |
151 |
en |
| dc.identifier.doi |
10.1016/j.vetmic.2011.03.008 |
en |
| dc.identifier.spage |
238 |
en |
| dc.identifier.epage |
244 |
en |