Impact of anaerobiosis strategy and bioreactor geometry on the biochemical response of Clostridium butyricum VPI 1718 during 1,3-propanediol fermentation

dc.contributor.author	Chatzifragkou, A	en
dc.contributor.author	Aggelis, G	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Zeng, A-P	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:51:21Z
dc.date.available	2014-06-06T06:51:21Z
dc.date.issued	2011	en
dc.identifier.issn	09608524	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.biortech.2011.09.023	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5466
dc.subject	1,3-Propanediol	en
dc.subject	Clostridium butyricum	en
dc.subject	Crude glycerol	en
dc.subject	Fed-batch	en
dc.subject	Lactate dehydrogenase	en
dc.subject.other	1 ,3 propanediol	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Crude glycerol	en
dc.subject.other	Fed batches	en
dc.subject.other	Lactate dehydrogenase	en
dc.subject.other	Bioreactors	en
dc.subject.other	Clostridium	en
dc.subject.other	Ecology	en
dc.subject.other	Fermentation	en
dc.subject.other	Geometry	en
dc.subject.other	Glycerol	en
dc.subject.other	Lactic acid	en
dc.subject.other	Metabolism	en
dc.subject.other	Synthesis (chemical)	en
dc.subject.other	Bioconversion	en
dc.subject.other	1,3 propanediol	en
dc.subject.other	glycerol	en
dc.subject.other	lactate dehydrogenase	en
dc.subject.other	lactic acid	en
dc.subject.other	nitrogen	en
dc.subject.other	anoxic conditions	en
dc.subject.other	biochemical composition	en
dc.subject.other	bioreactor	en
dc.subject.other	cultivation	en
dc.subject.other	enzyme activity	en
dc.subject.other	experimental study	en
dc.subject.other	fermentation	en
dc.subject.other	geometry	en
dc.subject.other	prokaryote	en
dc.subject.other	anaerobic growth	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial strain	en
dc.subject.other	biochemistry	en
dc.subject.other	bioreactor	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	coculture	en
dc.subject.other	enzyme activity	en
dc.subject.other	fed batch fermentation	en
dc.subject.other	geometry	en
dc.subject.other	nonhuman	en
dc.subject.other	priority journal	en
dc.subject.other	signal transduction	en
dc.subject.other	synthesis	en
dc.subject.other	Anaerobiosis	en
dc.subject.other	Batch Cell Culture Techniques	en
dc.subject.other	Biomass	en
dc.subject.other	Bioreactors	en
dc.subject.other	Biotechnology	en
dc.subject.other	Butyric Acid	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Fermentation	en
dc.subject.other	Glycerol	en
dc.subject.other	L-Lactate Dehydrogenase	en
dc.subject.other	Lactic Acid	en
dc.subject.other	Metabolic Networks and Pathways	en
dc.subject.other	Propylene Glycols	en
dc.subject.other	Time Factors	en
dc.subject.other	Clostridium butyricum	en
dc.title	Impact of anaerobiosis strategy and bioreactor geometry on the biochemical response of Clostridium butyricum VPI 1718 during 1,3-propanediol fermentation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.biortech.2011.09.023	en
heal.publicationDate	2011	en
heal.abstract	The impact of anaerobiosis strategy on 1,3-propanediol production during cultivation of Clostridium butyricum VPI 1718 in different size bioreactors was studied. In batch trials with N 2 gas infusion, the fermentation was successfully accomplished, regardless of initial glycerol concentration imposed and bioreactor geometry. However, in the absence of N 2 continual sparging, significant variations concerning the biochemical response of the strain were observed. Specifically, at 1-L bioreactor, the absence of N 2 infusion at high initial glycerol concentration induced lactate dehydrogenase activity and thus lactic acid synthesis, probably due to partial blockage of phosphoroclastic reaction caused by insufficient self-generated anaerobiosis environment. During fed-batch cultivation with continual N 2 sparging, the strain produced ∼71gL -1 of 1,3-propanediol, whereas under self-generated anaerobiosis, 1,3-propanediol pathway was evidently restricted, as only 30.5gL -1 of 1,3-propanediol were finally produced. Apparently, N 2 infusion strategy paired with bioreactor geometry can alter the biochemical behavior of the particular strain. © 2011 Elsevier Ltd.	en
heal.journalName	Bioresource Technology	en
dc.identifier.issue	22	en
dc.identifier.volume	102	en
dc.identifier.doi	10.1016/j.biortech.2011.09.023	en
dc.identifier.spage	10625	en
dc.identifier.epage	10632	en