Evaluation of growth/no growth interface of Listeria monocytogenes growing on stainless steel surfaces, detached from biofilms or in suspension, in response to pH and NaCl

dc.contributor.author	Belessi, CEA	en
dc.contributor.author	Gounadaki, AS	en
dc.contributor.author	Schvartzman, S	en
dc.contributor.author	Jordan, K	en
dc.contributor.author	Skandamis, PN	en
dc.date.accessioned	2014-06-06T06:51:19Z
dc.date.available	2014-06-06T06:51:19Z
dc.date.issued	2011	en
dc.identifier.issn	01681605	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.ijfoodmicro.2010.10.031	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5448
dc.subject	Attached	en
dc.subject	Biofilms	en
dc.subject	Detached	en
dc.subject	Growth/no growth interface	en
dc.subject	Listeria monocytogenes	en
dc.subject	NaCl	en
dc.subject	PH	en
dc.subject	Planktonic	en
dc.subject.other	sodium chloride	en
dc.subject.other	stainless steel	en
dc.subject.other	article	en
dc.subject.other	bacterial colonization	en
dc.subject.other	bacterial growth	en
dc.subject.other	bacterial strain	en
dc.subject.other	bacterial survival	en
dc.subject.other	biofilm	en
dc.subject.other	colony forming unit	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	nonhuman	en
dc.subject.other	pH	en
dc.subject.other	probability	en
dc.subject.other	Biofilms	en
dc.subject.other	Food Microbiology	en
dc.subject.other	Hydrogen-Ion Concentration	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Sodium Chloride	en
dc.subject.other	Stainless Steel	en
dc.subject.other	Suspensions	en
dc.subject.other	Listeria monocytogenes	en
dc.title	Evaluation of growth/no growth interface of Listeria monocytogenes growing on stainless steel surfaces, detached from biofilms or in suspension, in response to pH and NaCl	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijfoodmicro.2010.10.031	en
heal.publicationDate	2011	en
heal.abstract	The present study aimed to describe the growth/no growth interface of Listeria monocytogenes at three potential states of growth in industrial environments, namely attached, (Att), detached (Det) from a biofilm, or in a planktonic state (suspended; Plan). A 3-strain composite of L. monocytogenes cells was left to colonize stainless steel (SS) surfaces in tryptic soy broth supplemented with 0.6% yeast extract (TSBYE) at 20°C for 72h and then transferred to TSBYE at 30 different pH and NaCl concentrations, which were renewed every two days during incubation at 10°C. Survival of attached population was observed at optimal conditions (pH 7.2, a w 0.996), whereas at 4.5-8.0% salt and/or pH<6.0, reduction of attached population on SS surfaces was observed. PFGE patterns showed that 91% of the cells colonizing the SS coupons after 30days, at any pH and a w conditions, belonged to a single strain. Furthermore, the change in the probability of a single cell to initiate growth (P in) over time, as well as the number of cells needed (CN) for growth initiation of planktonically growing Plan and Det L. monocytogenes cells were evaluated based on MPN Tables. An ordinary logistic regression model was also used to describe the growth/no growth interface of varying inoculation levels (from <10 to 10 4CFU/ml) of Plan and Det cells in response to pH and a w. Although both cell types demonstrated similar growth limits at populations of 10 2-10 4CFU/ml, higher numbers of Det than Plan cells were needed (CN) in order to initiate growth at low a w and pH. Individual Plan cells reached higher maximum levels of probability of growth initiation (P max) and had shorter times to reach P max/2 (t au), compared to their Det counterparts. Data on growth potential of cells in suspension, attached or detached status, may assist in ranking the risk from different sources of contamination. In addition, they may establish the link between the behavior of L. monocytogenes in foods and its origin from the processing plant. The latter link is important component of biotraceability. © 2010 Elsevier B.V.	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.issue	SUPPL. 1	en
dc.identifier.volume	145	en
dc.identifier.doi	10.1016/j.ijfoodmicro.2010.10.031	en
dc.identifier.spage	S53	en
dc.identifier.epage	S60	en