Efficiency of different sanitation methods on Listeria monocytogenes biofilms formed under various environmental conditions

dc.contributor.author	Belessi, CEA	en
dc.contributor.author	Gounadaki, AS	en
dc.contributor.author	Psomas, AN	en
dc.contributor.author	Skandamis, PN	en
dc.date.accessioned	2014-06-06T06:51:18Z
dc.date.available	2014-06-06T06:51:18Z
dc.date.issued	2011	en
dc.identifier.issn	01681605	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.ijfoodmicro.2010.10.020	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5437
dc.subject	Acid adaptation	en
dc.subject	Attached	en
dc.subject	Biofilms	en
dc.subject	Detached	en
dc.subject	Listeria monocytogenes	en
dc.subject	Planktonic	en
dc.subject	Sanitizers	en
dc.subject.other	chlorine	en
dc.subject.other	peracetic acid	en
dc.subject.other	quaternary ammonium derivative	en
dc.subject.other	stainless steel	en
dc.subject.other	article	en
dc.subject.other	bacterial survival	en
dc.subject.other	bacterium adherence	en
dc.subject.other	bacterium culture	en
dc.subject.other	biofilm	en
dc.subject.other	culture medium	en
dc.subject.other	disinfection	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	nonhuman	en
dc.subject.other	sanitation	en
dc.subject.other	Biofilms	en
dc.subject.other	Chlorine	en
dc.subject.other	Disinfectants	en
dc.subject.other	Disinfection	en
dc.subject.other	Environment	en
dc.subject.other	Food Handling	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Peracetic Acid	en
dc.subject.other	Quaternary Ammonium Compounds	en
dc.subject.other	Sodium Chloride	en
dc.subject.other	Stainless Steel	en
dc.subject.other	Temperature	en
dc.subject.other	Water	en
dc.subject.other	Listeria monocytogenes	en
dc.subject.other	Paa	en
dc.title	Efficiency of different sanitation methods on Listeria monocytogenes biofilms formed under various environmental conditions	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijfoodmicro.2010.10.020	en
heal.publicationDate	2011	en
heal.abstract	The resistance of Listeria monocytogenes biofilms formed under food processing conditions, against various sanitizing agents and disinfection procedures was evaluated in the present study. The first sanitation procedure included biofilm formation on stainless steel coupons (SS) placed in tryptic soy broth supplemented with 0.6% yeast extract (TSBYE) of various concentrations of NaCl (0.5, 7.5 and 9.5%) at different temperatures (5 and 20°C). The biofilms formed were exposed to warm (60°C) water for 20min, or to peroxyacetic acid (2% PAA) for 1, 2, 3 and 6min. Treatment with warm water caused no significant (P≥0.05) reductions in the attached populations. Conversely, surviving bacteria on SS coupons decreased as the exposure time to 2% PAA increased and could not be detected by culture after 6min of exposure. Biofilms formed at 20°C were more resistant to PAA than biofilms formed at 5°C. Salt concentration in the growth medium had no marked impact on the resistance to PAA. The second sanitation procedure included biofilm formation of nonadapted (NA) and acid-adapted (AA) cells in TSBYE of pH 5.0 and 7.0 (i.e., NA-5.0, NA-7.0 and AA-5.0, AA-7.0) at 4°C. Coupons bearing attached cells of L. monocytogenes were periodically exposed to chlorine (0.465% Cl -), quaternary ammonium compound (1% QAC) and 2% PAA. The resistance of attached cells to QAC, PAA and Cl - followed the order: AA-5.0>NA-7.0≥AA-7.0>NA-5.0. The most effective sanitizer was QAC followed by PAA and Cl -. The results can lead to the development of efficient sanitation strategies in order to eliminate L. monocytogenes from the processing environment. Furthermore, such results may explain the presence of L. monocytogenes after sanitation as a result of cell attachment history. © 2010 Elsevier B.V.	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.issue	SUPPL. 1	en
dc.identifier.volume	145	en
dc.identifier.doi	10.1016/j.ijfoodmicro.2010.10.020	en
dc.identifier.spage	S46	en
dc.identifier.epage	S52	en