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Effect of Origanum vulgare L. essential oil on growth and lipid profile of Yarrowia lipolytica cultivated on glycerol-based media

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dc.contributor.author Chatzifragkou, A en
dc.contributor.author Petrou, I en
dc.contributor.author Gardeli, C en
dc.contributor.author Komaitis, M en
dc.contributor.author Papanikolaou, S en
dc.date.accessioned 2014-06-06T06:51:17Z
dc.date.available 2014-06-06T06:51:17Z
dc.date.issued 2011 en
dc.identifier.issn 0003021X en
dc.identifier.uri http://dx.doi.org/10.1007/s11746-011-1870-4 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5429
dc.subject Essential oil en
dc.subject Lipids en
dc.subject Origanum vulgare L en
dc.subject Raw glycerol en
dc.subject Yarrowia lipolytica en
dc.subject.other Chemical compositions en
dc.subject.other Culture medium en
dc.subject.other GC-MS analysis en
dc.subject.other Lipid composition en
dc.subject.other Lipid profile en
dc.subject.other Low concentrations en
dc.subject.other Origanum vulgare en
dc.subject.other Raw glycerol en
dc.subject.other Shake-flask cultivation en
dc.subject.other Y. lipolytica en
dc.subject.other Yarrowia lipolytica en
dc.subject.other Glycerol en
dc.subject.other Lipids en
dc.subject.other Phenols en
dc.subject.other Saturated fatty acids en
dc.subject.other Yeast en
dc.subject.other Essential oils en
dc.subject.other Origanum en
dc.subject.other Origanum vulgare en
dc.subject.other Yarrowia lipolytica en
dc.title Effect of Origanum vulgare L. essential oil on growth and lipid profile of Yarrowia lipolytica cultivated on glycerol-based media en
heal.type journalArticle en
heal.identifier.primary 10.1007/s11746-011-1870-4 en
heal.publicationDate 2011 en
heal.abstract Aim of the present study was to identify the chemical composition of Origanum vulgare L. essential oil and to investigate its effect on the biochemical behavior of the yeast Yarrowia lipolytica. Twenty-three compounds were identified by GC-MS analysis, representing the 92.3% (w/w) of the total essential oil of the plant. Carvacrol (56.3%) and thymol (16.4%) were the major components. Additionally, shake-flask cultivations of Y. lipolytica were performed, with various essential oil additions (0.05-2 mL/L of medium) on glycerol-based media. Growth was affected even at low concentrations (0.05 mL/L), while in higher essential oil concentrations, strong inhibition phenomena were observed. A tolerance-threshold concentration for the strain was hence established at 0.15 mL/L of oil. Furthermore, the presence of the essential oil in the culture medium resulted in changes in the composition of the intra-cellular lipids of the yeast. Specifically, oil addition to nitrogen-limited cultures to a level >0.15 mL/L caused a substantial increase in the percentage of saturated fatty acids (C16:0 and C18:0) in the lipid composition of the yeast Y. lipolytica. © AOCS 2011. en
heal.journalName JAOCS, Journal of the American Oil Chemists' Society en
dc.identifier.issue 12 en
dc.identifier.volume 88 en
dc.identifier.doi 10.1007/s11746-011-1870-4 en
dc.identifier.spage 1955 en
dc.identifier.epage 1964 en


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