Development of an IC-RT-PCR assay for the detection of Eggplant mottled dwarf virus and partial characterization of isolates from various hosts in Greece

dc.contributor.author	Katis, NI	en
dc.contributor.author	Chatzivassiliou, EK	en
dc.contributor.author	Clay, CM	en
dc.contributor.author	Maliogka, VI	en
dc.contributor.author	Pappi, P	en
dc.contributor.author	Efthimiou, K	en
dc.contributor.author	Dovas, CI	en
dc.contributor.author	Avgelis, AD	en
dc.date.accessioned	2014-06-06T06:51:16Z
dc.date.available	2014-06-06T06:51:16Z
dc.date.issued	2011	en
dc.identifier.issn	11254653	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5419
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-84856294925&partnerID=40&md5=8ac756f5be9707c5c19b772bc5552ec2	en
dc.subject	Cytopathology	en
dc.subject	Electron microscopy	en
dc.subject	EMDV	en
dc.subject	IC-RT-PCR detection	en
dc.subject	Western blot	en
dc.subject.other	Capparis	en
dc.subject.other	Capparis spinosa	en
dc.subject.other	Cucumis sativus	en
dc.subject.other	Nicotiana tabacum	en
dc.subject.other	Pittosporum tobira	en
dc.subject.other	Solanum melongena	en
dc.title	Development of an IC-RT-PCR assay for the detection of Eggplant mottled dwarf virus and partial characterization of isolates from various hosts in Greece	en
heal.type	journalArticle	en
heal.publicationDate	2011	en
heal.abstract	In a 10-year survey several Eggplant mottled dwarf virus (EMDV) isolates were collected from diverse hosts from all over Greece. In this study, an IC-RT-PCR assay was developed for the detection of the virus. Degenerate primers were designed on conserved regions of the glycoprotein (G) gene of various plant nucleorhabdoviruses. Based on the initial genetic information obtained, EMDV-specific primers were subsequently designed. The method successfully amplified a 284 bp product from all (76) virus samples originating from tobacco, cucumber, eggplant, Pittosporum tobira and Capparis spinosa (caper). Sequence analysis and Western blot were done for further characterization of representative isolates. Two isolates from tobacco (EMDV-Tob) and cucumber (EMDV-Cu) were also subjected to electron microscopy and host range studies. Sequence analysis of the partial G gene confirmed the classification of EMDV within nucleorhabdoviruses. Genetic comparisons among the EMDV isolates tested indicated low levels of variation with the caper isolate, which is reported for the first time in Greece, being the most deviating isolate. This is the first molecular assay developed for the detection of EMDV and also the first sequence information obtained of its genome.	en
heal.journalName	Journal of Plant Pathology	en
dc.identifier.issue	2	en
dc.identifier.volume	93	en
dc.identifier.spage	353	en
dc.identifier.epage	362	en