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Citric acid, biomass and cellular lipid production by Yarrowia lipolytica strains cultivated on olive mill wastewater-based media

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dc.contributor.author Sarris, D en
dc.contributor.author Galiotou-Panayotou, M en
dc.contributor.author Koutinas, AA en
dc.contributor.author Komaitis, M en
dc.contributor.author Papanikolaou, S en
dc.date.accessioned 2014-06-06T06:51:14Z
dc.date.available 2014-06-06T06:51:14Z
dc.date.issued 2011 en
dc.identifier.issn 02682575 en
dc.identifier.uri http://dx.doi.org/10.1002/jctb.2658 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5399
dc.subject Cellular lipids en
dc.subject Citric acid en
dc.subject Olive mill wastewaters en
dc.subject Waste bioremediation en
dc.subject Yarrowia lipolytica en
dc.subject.other Biomass concentrations en
dc.subject.other Biomass conversion en
dc.subject.other Biomass productions en
dc.subject.other Cellular lipid en
dc.subject.other Conversion yield en
dc.subject.other In-control en
dc.subject.other Inhibitory compounds en
dc.subject.other Olive mill en
dc.subject.other Olive mill wastewaters en
dc.subject.other Physico-chemicals en
dc.subject.other Removal of phenolic compounds en
dc.subject.other Shake-flask cultures en
dc.subject.other Waste bioremediation en
dc.subject.other Y. lipolytica en
dc.subject.other Yarrowia lipolytica en
dc.subject.other Bioconversion en
dc.subject.other Biomass en
dc.subject.other Bioremediation en
dc.subject.other Biotechnology en
dc.subject.other Glucose en
dc.subject.other Lipids en
dc.subject.other Oleic acid en
dc.subject.other Phenols en
dc.subject.other Wastewater en
dc.subject.other Wastewater treatment en
dc.subject.other Citric acid en
dc.subject.other carbon en
dc.subject.other citric acid en
dc.subject.other glucose en
dc.subject.other lipid en
dc.subject.other nitrogen en
dc.subject.other phenol derivative en
dc.subject.other sugar en
dc.subject.other article en
dc.subject.other biomass en
dc.subject.other biomass conversion en
dc.subject.other biomass production en
dc.subject.other concentration (parameters) en
dc.subject.other controlled study en
dc.subject.other culture medium en
dc.subject.other decolorization en
dc.subject.other fatty acid analysis en
dc.subject.other fungal strain en
dc.subject.other fungus growth en
dc.subject.other lipid storage en
dc.subject.other nonhuman en
dc.subject.other olive en
dc.subject.other olive mill wastewater en
dc.subject.other waste water en
dc.subject.other Yarrowia lipolytica en
dc.subject.other Yarrowia lipolytica en
dc.title Citric acid, biomass and cellular lipid production by Yarrowia lipolytica strains cultivated on olive mill wastewater-based media en
heal.type journalArticle en
heal.identifier.primary 10.1002/jctb.2658 en
heal.publicationDate 2011 en
heal.abstract Background: Olive mill wastewaters (OMWs) are an important residue and several physico-chemical and/or biotechnological methods have been proposed for their treatment. Results: The ability of three Yarrowia lipolytica strains to grow on and convert glucose-enriched OMWs into added-value compounds in carbon- and nitrogen-limited shake-flask cultures was assessed. Remarkable decolorization (up to 63%) and non-negligible removal of phenolic compounds (up to 34%, w/w) occurred. In nitrogen-limited cultures, the accumulation of cellular lipids was favored by OMW addition into the medium. In contrast, although remarkable quantities of citric acid (Cit) were produced in control experiments (cultures without OMW addition), in which Cit up to 18.9 g L-1 was produced with yield of Cit synthesized per sugar consumed ∼0.73 g g-1), adaptation of cultures to media supplemented with OMWs reduced the final Cit quantity and conversion yield values achieved. In OMW-based media, the highest concentration of citric acid produced was 18.1 g L-1, with conversion yield ∼0.51 g g-1. In carbon-limited cultures, despite the presence of inhibitory compounds in the medium (e.g. phenols), biomass production was enhanced with the addition of OMWs. The highest biomass concentration achieved was 12.7 g L-1, with biomass conversion yield per sugar consumed ∼0.45 g g-1. Fatty acid analysis of cellular lipid produced demonstrated that adaptation of all strains in OMW-based media favored the synthesis of cellular lipids that contained increased concentrations of cellular oleic acid. Conclusions: The Y. lipolytica strains tested can be regarded as possible candidates for simultaneous OMWs remediation and production of added-value compounds. © 2011 Society of Chemical Industry. en
heal.journalName Journal of Chemical Technology and Biotechnology en
dc.identifier.issue 11 en
dc.identifier.volume 86 en
dc.identifier.doi 10.1002/jctb.2658 en
dc.identifier.spage 1439 en
dc.identifier.epage 1448 en


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