| dc.contributor.author |
Tsikou, D |
en |
| dc.contributor.author |
Stedel, C |
en |
| dc.contributor.author |
Kouri, ED |
en |
| dc.contributor.author |
Udvardi, MK |
en |
| dc.contributor.author |
Wang, TL |
en |
| dc.contributor.author |
Katinakis, P |
en |
| dc.contributor.author |
Labrou, NE |
en |
| dc.contributor.author |
Flemetakis, E |
en |
| dc.date.accessioned |
2014-06-06T06:51:13Z |
|
| dc.date.available |
2014-06-06T06:51:13Z |
|
| dc.date.issued |
2011 |
en |
| dc.identifier.issn |
15709639 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.bbapap.2011.01.007 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5398 |
|
| dc.subject |
Carbonic anhydrase |
en |
| dc.subject |
Lotus japonicus |
en |
| dc.subject |
Nodule |
en |
| dc.subject |
Symbiotic nitrogen fixation |
en |
| dc.subject.other |
carbonate dehydratase |
en |
| dc.subject.other |
complementary DNA |
en |
| dc.subject.other |
isoenzyme |
en |
| dc.subject.other |
vegetable protein |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
down regulation |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
gene expression profiling |
en |
| dc.subject.other |
legume |
en |
| dc.subject.other |
LjCAA1 gene |
en |
| dc.subject.other |
LjCAA2 gene |
en |
| dc.subject.other |
Lotus japonicus |
en |
| dc.subject.other |
Mesorhizobium |
en |
| dc.subject.other |
mutant |
en |
| dc.subject.other |
nitrogen fixation |
en |
| dc.subject.other |
nodulation |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
physiological process |
en |
| dc.subject.other |
phytochemistry |
en |
| dc.subject.other |
plant cell |
en |
| dc.subject.other |
plant gene |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
protein expression |
en |
| dc.subject.other |
protein purification |
en |
| dc.subject.other |
vascular bundle (plant) |
en |
| dc.subject.other |
Amino Acid Sequence |
en |
| dc.subject.other |
Carbonic Anhydrases |
en |
| dc.subject.other |
DNA, Complementary |
en |
| dc.subject.other |
Enzyme Assays |
en |
| dc.subject.other |
Gene Expression Regulation, Plant |
en |
| dc.subject.other |
Lotus |
en |
| dc.subject.other |
Models, Molecular |
en |
| dc.subject.other |
Molecular Sequence Data |
en |
| dc.subject.other |
Phylogeny |
en |
| dc.subject.other |
Protein Structure, Secondary |
en |
| dc.subject.other |
RNA, Messenger |
en |
| dc.subject.other |
Root Nodules, Plant |
en |
| dc.subject.other |
Sequence Homology, Amino Acid |
en |
| dc.subject.other |
Up-Regulation |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
Lotus corniculatus var. japonicus |
en |
| dc.subject.other |
Mesorhizobium loti |
en |
| dc.title |
Characterization of two novel nodule-enhanced α-type carbonic anhydrases from Lotus japonicus |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.bbapap.2011.01.007 |
en |
| heal.publicationDate |
2011 |
en |
| heal.abstract |
Two cDNA clones coding for α-type carbonic anhydrases (CA; EC 4.2.1.1) in the nitrogen-fixing nodules of the model legume Lotus japonicus were identified. Functionality of the full-length proteins was confirmed by heterologous expression in Escherichia coli and purification of the encoded polypeptides. The developmental expression pattern of LjCAA1 and LjCAA2 revealed that both genes code for nodule enhanced carbonic anhydrase isoforms, which are induced early during nodule development. The genes were slightly to moderately down-regulated in ineffective nodules formed by mutant Mesorhizobium loti strains, indicating that these genes may also be involved in biochemical and physiological processes not directly linked to nitrogen fixation/assimilation. The spatial expression profiling revealed that both genes were expressed in nodule inner cortical cells, vascular bundles and central tissue. These results are discussed in the context of the possible roles of CA in nodule carbon dioxide (CO 2) metabolism. © 2011 Elsevier B.V. All rights reserved. |
en |
| heal.journalName |
Biochimica et Biophysica Acta - Proteins and Proteomics |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
1814 |
en |
| dc.identifier.doi |
10.1016/j.bbapap.2011.01.007 |
en |
| dc.identifier.spage |
496 |
en |
| dc.identifier.epage |
504 |
en |