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Biotechnological valorization of low-cost sugar-based media for bacteriocin production by Leuconostoc mesenteroides E131

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dc.contributor.author Metsoviti, M en
dc.contributor.author Paramithiotis, S en
dc.contributor.author Drosinos, EH en
dc.contributor.author Skandamis, PN en
dc.contributor.author Galiotou-Panayotou, M en
dc.contributor.author Papanikolaou, S en
dc.date.accessioned 2014-06-06T06:51:13Z
dc.date.available 2014-06-06T06:51:13Z
dc.date.issued 2011 en
dc.identifier.issn 18716784 en
dc.identifier.uri http://dx.doi.org/10.1016/j.nbt.2011.03.004 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5391
dc.subject.other Bacteriocin production en
dc.subject.other Bacteriocins en
dc.subject.other Carbon source en
dc.subject.other Electron acceptor en
dc.subject.other Initial concentration en
dc.subject.other Leuconostoc mesenteroides en
dc.subject.other Shake-flask experiments en
dc.subject.other Bacilli en
dc.subject.other Detoxification en
dc.subject.other Ethanol en
dc.subject.other Fructose en
dc.subject.other Glucose en
dc.subject.other Sugar (sucrose) en
dc.subject.other Sugar factories en
dc.subject.other Carbon en
dc.subject.other acetic acid en
dc.subject.other alcohol en
dc.subject.other bacteriocin en
dc.subject.other fructose en
dc.subject.other glucose en
dc.subject.other lactic acid en
dc.subject.other sucrose en
dc.subject.other sugar en
dc.subject.other article en
dc.subject.other biomass en
dc.subject.other biotechnology en
dc.subject.other decolorization en
dc.subject.other kinetics en
dc.subject.other Leuconostoc mesenteroides en
dc.subject.other microorganism en
dc.subject.other nonhuman en
dc.subject.other priority journal en
dc.subject.other Bacteriocins en
dc.subject.other Culture Media en
dc.subject.other Fructose en
dc.subject.other Glucose en
dc.subject.other Leuconostoc en
dc.subject.other Molasses en
dc.subject.other Sucrose en
dc.subject.other Sweetening Agents en
dc.subject.other Leuconostoc mesenteroides en
dc.title Biotechnological valorization of low-cost sugar-based media for bacteriocin production by Leuconostoc mesenteroides E131 en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.nbt.2011.03.004 en
heal.publicationDate 2011 en
heal.abstract The aim of the present study was to evaluate the suitability of low-cost carbon sources for bacteriocin production by Leuconostoc mesenteroides strain E131. For this purpose, inexpensive sugars derived from a sugar refinery plant (glucose, fructose and sucrose) as well as waste molasses were utilized as carbon sources in submerged shake-flask experiments and the kinetic response of the microorganism was evaluated. Interestingly, in the case of molasses, non-negligible decolorization-detoxification (up to ~27%) of the residue was performed together with the production of bacteriocin. In all instances the initial concentration of sugars employed was adjusted at 20 and 30. g/L, therefore the effect of both the nature and the initial quantity of sugar upon the growth of the microorganism was assessed. All media proved to be suitable for both biomass and bacteriocin production by L. mesenteroides, whereas variable quantities of lactate, acetate and ethanol were detected into the medium. Employment of fructose, sucrose or molasses as carbon sources resulted in the accumulation of mannitol (in some cases in significant quantities) into the medium; remarkable portion thus of the available or released fructose acted as electron acceptor instead of carbon source by the microorganism. The highest bacteriocin production achieved (=640. AU/mL) was obtained when initial glucose at 30. g/L was used as substrate. Finally, utilization of waste molasses as carbon source by L. mesenteroides resulted in satisfactory bacteriocin production (up to 320. AU/mL) besides the decolorization of the residue. © 2011 Elsevier B.V. en
heal.journalName New Biotechnology en
dc.identifier.issue 6 en
dc.identifier.volume 28 en
dc.identifier.doi 10.1016/j.nbt.2011.03.004 en
dc.identifier.spage 600 en
dc.identifier.epage 609 en


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