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Bacterial species associated with sound and Botrytis-infected grapes from a Greek vineyard

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dc.contributor.author Nisiotou, AA en
dc.contributor.author Rantsiou, K en
dc.contributor.author Iliopoulos, V en
dc.contributor.author Cocolin, L en
dc.contributor.author Nychas, GJE en
dc.date.accessioned 2014-06-06T06:51:12Z
dc.date.available 2014-06-06T06:51:12Z
dc.date.issued 2011 en
dc.identifier.issn 01681605 en
dc.identifier.uri http://dx.doi.org/10.1016/j.ijfoodmicro.2011.01.017 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5387
dc.subject Bacteria en
dc.subject Botrytis en
dc.subject Grape must fermentation en
dc.subject Grapes en
dc.subject Wine en
dc.subject.other ribosome RNA en
dc.subject.other article en
dc.subject.other Bacillus subtilis en
dc.subject.other bacterium isolate en
dc.subject.other Botrytis en
dc.subject.other Citrobacter freundii en
dc.subject.other controlled study en
dc.subject.other cultivar en
dc.subject.other denaturing gradient gel electrophoresis en
dc.subject.other Enterobacter en
dc.subject.other Enterobacter ludwigii en
dc.subject.other Enterobacteriaceae en
dc.subject.other Erwinia en
dc.subject.other fermentation en
dc.subject.other gene sequence en
dc.subject.other Gluconobacter cerinus en
dc.subject.other grape en
dc.subject.other Klebsiella oxytoca en
dc.subject.other Lactobacillus plantarum en
dc.subject.other mycosis en
dc.subject.other nonhuman en
dc.subject.other nucleotide sequence en
dc.subject.other Pantoea dispersa en
dc.subject.other polymerase chain reaction en
dc.subject.other Providencia rettgeri en
dc.subject.other Serratia marcescens en
dc.subject.other Staphylococcus epidermidis en
dc.subject.other tatumella ptyseos en
dc.subject.other vineyard en
dc.subject.other Botrytis en
dc.subject.other Colony Count, Microbial en
dc.subject.other Denaturing Gradient Gel Electrophoresis en
dc.subject.other Enterobacteriaceae en
dc.subject.other Fermentation en
dc.subject.other Food Microbiology en
dc.subject.other Plant Diseases en
dc.subject.other Polymerase Chain Reaction en
dc.subject.other RNA, Ribosomal, 16S en
dc.subject.other Vitis en
dc.subject.other Wine en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Botrytis en
dc.subject.other Citrobacter freundii en
dc.subject.other Enterobacter en
dc.subject.other Enterobacter ludwigii en
dc.subject.other Enterobacteriaceae en
dc.subject.other Erwinia sp. en
dc.subject.other Klebsiella oxytoca en
dc.subject.other Lactobacillus plantarum en
dc.subject.other Pantoea dispersa en
dc.subject.other Tatumella ptyseos en
dc.subject.other Vitaceae en
dc.title Bacterial species associated with sound and Botrytis-infected grapes from a Greek vineyard en
heal.type journalArticle en
heal.identifier.primary 10.1016/j.ijfoodmicro.2011.01.017 en
heal.publicationDate 2011 en
heal.abstract Grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. In the present study, bacterial species associated with sound and Botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. Isolates were identified by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and sequence analysis of partial 16S rRNA gene. Comparable counts were recorded between Botrytis-infected and sound grape samples. In all cases, the majority of isolates belonged to different species of Enterobacteriaceae. The dominant species in the vineyard was Klebsiella oxytoca that was found in different combinations with Citrobacter freundii, Enterobacter spp., Erwinia sp., Pantoea dispersa, Tatumella ptyseos or other species. In fermenting musts, those populations declined while other species evolved, like Lactobacillus plantarum and Enterobacter ludwigii. Populations in botrytised samples persisted longer during spontaneous fermentations. Present study suggests that bacterial diversity on grapes may be wider than previously described. © 2011 Elsevier B.V. en
heal.journalName International Journal of Food Microbiology en
dc.identifier.issue 2-3 en
dc.identifier.volume 145 en
dc.identifier.doi 10.1016/j.ijfoodmicro.2011.01.017 en
dc.identifier.spage 432 en
dc.identifier.epage 436 en


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