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Autoinducer-2-like activity in lactic acid bacteria isolated from minced beef packaged under modified atmospheres

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dc.contributor.author Blana, VA en
dc.contributor.author Doulgeraki, AI en
dc.contributor.author Nychas, G-JE en
dc.date.accessioned 2014-06-06T06:51:12Z
dc.date.available 2014-06-06T06:51:12Z
dc.date.issued 2011 en
dc.identifier.issn 0362028X en
dc.identifier.uri http://dx.doi.org/10.4315/0362-028X.JFP-10-276 en
dc.identifier.uri http://62.217.125.90/xmlui/handle/123456789/5386
dc.subject.other drug derivative en
dc.subject.other homoserine en
dc.subject.other lactone en
dc.subject.other n octanoylhomoserine lactone en
dc.subject.other N-octanoylhomoserine lactone en
dc.subject.other animal en
dc.subject.other article en
dc.subject.other bacterial count en
dc.subject.other cattle en
dc.subject.other food contamination en
dc.subject.other food control en
dc.subject.other food packaging en
dc.subject.other genetics en
dc.subject.other isolation and purification en
dc.subject.other Lactobacillales en
dc.subject.other meat en
dc.subject.other metabolism en
dc.subject.other methodology en
dc.subject.other microbiology en
dc.subject.other product safety en
dc.subject.other temperature en
dc.subject.other Animals en
dc.subject.other Cattle en
dc.subject.other Colony Count, Microbial en
dc.subject.other Consumer Product Safety en
dc.subject.other Food Contamination en
dc.subject.other Food Microbiology en
dc.subject.other Food Packaging en
dc.subject.other Homoserine en
dc.subject.other Lactobacillales en
dc.subject.other Lactones en
dc.subject.other Meat Products en
dc.subject.other Temperature en
dc.subject.other Bacteria (microorganisms) en
dc.subject.other Lactobacillus sakei en
dc.subject.other Leuconostoc en
dc.subject.other Leuconostoc citreum en
dc.subject.other Leuconostoc mesenteroides en
dc.subject.other Leuconostoc sakei en
dc.subject.other Vibrio harveyi en
dc.subject.other Weissella viridescens en
dc.title Autoinducer-2-like activity in lactic acid bacteria isolated from minced beef packaged under modified atmospheres en
heal.type journalArticle en
heal.identifier.primary 10.4315/0362-028X.JFP-10-276 en
heal.publicationDate 2011 en
heal.abstract Fifteen fingerprints (assigned to Leuconostoc spp., Leuconostoc mesenteroides, Weissella viridescens, Leuconostoc citreum, and Lactobacillus sakei) of 89 lactic acid bacteria (LAB) isolated from minced beef stored under modified atmospheres at various temperatures were screened for their ability to exhibit autoinducer-2 (AI-2)-like activity under certain growth conditions. Cellfree meat extracts (CFME) were collected at the same time as the LAB isolates and tested for the presence of AI-2-like molecules. All bioassays were conducted using the Vibrio harveyi BAA-1117 (sensor 1-, sensor 2 +) biosensor strain. The possible inhibitory effect of meat extracts on the activity of the biosensor strain was also evaluated. AI-2-like activity was observed for Leuconostoc spp. isolates, but none of the L. sakei strains produced detectable AI-2-like activity. The AI-2-like activity was evident mainly associated with the Leuconostoc sp. B 233 strain, which was the dominant isolate recovered from storage at 10 and 15°C and at the initial and middle stages of storage at chill temperatures (0 and 5°C). The tested CFME samples displayed low AI-2-like activity and inhibited AI-2 activity regardless of the indigenous bacterial populations. The LAB isolated during meat spoilage exhibited AI-2-like activity, whereas the LAB strains retrieved depended on storage time and temperature. The production of AI-2-like molecules may affect the dominance of different bacterial strains during storage. The results provide a basis for further research concerning the effect of storage temperature on the expression of genes encoding AI-2 activity and on the diversity of the ephemeral bacterial population. Copyright © International Association for Food Protection. en
heal.journalName Journal of Food Protection en
dc.identifier.issue 4 en
dc.identifier.volume 74 en
dc.identifier.doi 10.4315/0362-028X.JFP-10-276 en
dc.identifier.spage 631 en
dc.identifier.epage 635 en


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