Achievement of thousand-fold accumulation of rosmarinic acid in immobilized cells of sweet basil (Ocimum basilicum L.) by ten-fold increase of the volume of the immobilization matrix

dc.contributor.author	Moschopoulou, G	en
dc.contributor.author	Kintzios, S	en
dc.date.accessioned	2014-06-06T06:51:10Z
dc.date.available	2014-06-06T06:51:10Z
dc.date.issued	2011	en
dc.identifier.issn	1790045X	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5368
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-79953713227&partnerID=40&md5=bbe100f38a1d7c8d65c6691882b403dc	en
dc.subject	Calcium alginate	en
dc.subject	Immobilization	en
dc.subject	Ocimum basilicum	en
dc.subject	Rosmarinic acid	en
dc.subject.other	Ocimum	en
dc.subject.other	Ocimum basilicum	en
dc.title	Achievement of thousand-fold accumulation of rosmarinic acid in immobilized cells of sweet basil (Ocimum basilicum L.) by ten-fold increase of the volume of the immobilization matrix	en
heal.type	journalArticle	en
heal.publicationDate	2011	en
heal.abstract	A very high rosmarinic acid (RA) production (20 mg g-1 dry weight) was achieved by immobilizing sweet basil (Ocimum basilicum L.) cells at a high density (approximately 25×104 cells ml-1) in a 4-ml gel matrix, a production performance that was 1400 times higher than in basil cells immobilized in spherical beads, with a 14-fold lower volume. Therefore, doubling of the volume of the immobilization matrix was associated with an increase of RA accumulation by a hundred times. In addition, RA was excreted into the culture medium, where it was collected without terminating the culture of immobilized cells. A complex relationship between oxygen transfer, ascorbate and RA biosynthesis was observed, with L-phenylalanine ammonia lyase (PAL) activity appearing to be a critical common link. The results are discussed in consideration of stress under conditions of nutrient and oxygen transfer restriction to immobilized cells.	en
heal.journalName	Journal of Biological Research	en
dc.identifier.volume	15	en
dc.identifier.spage	59	en
dc.identifier.epage	65	en