Detection of Pathogenic Mycobacteria Based on Functionalized Quantum Dots Coupled with Immunomagnetic Separation

dc.contributor.advisor	Liandris, Emmanouil	en
dc.contributor.advisor	Λιανδρής, Εμμανουήλ	el
dc.contributor.author	Gazouli, Maria	en
dc.contributor.author	Andreadou, Margarita	en
dc.contributor.author	Sechi, Leonardo A.	it
dc.contributor.author	Rosu, Valentina	it
dc.contributor.author	Ikonomopoulos, John A.	en
dc.contributor.author	Οικονομόπουλος, Ιωάννης Α.	el
dc.contributor.author	Γκαζούλη, Μαρία	el
dc.contributor.author	Ανδρεάδου, Μαργαρίτα	el
dc.date.accessioned	2014-06-06T06:51:08Z
dc.date.available	2014-06-06T06:51:08Z
dc.date.issued	2011-05-27	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0020026	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5341
dc.rights	CC0 1.0 Παγκόσμια	en
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.subject	Detection limit	en
dc.subject	Magnetic bead	en
dc.subject	Monoclonal antibody	en
dc.subject	Mycobacterium bovis	en
dc.subject	Mycobacterium tuberculosis	en
dc.subject	Polyclonal antibody	en
dc.subject	Protein targeting	en
dc.subject	Salmonella	en
dc.subject	Quantum dots	en
dc.title	Detection of Pathogenic Mycobacteria Based on Functionalized Quantum Dots Coupled with Immunomagnetic Separation	en
heal.type	journalArticle	en
heal.identifier.primary	10.1371/journal.pone.0020026	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Ζωικής Παραγωγής	el
heal.recordProvider	Πανεπιστήμιο Αθηνών/Ιατρική Σχολή	el
heal.recordProvider	University of Sassari/Biomedical Science	en
heal.publicationDate	2011-05-27	en
heal.bibliographicCitation	Liandris, Emmanouil. Detection of Pathogenic Mycobacteria Based on Functionalized Quantum Dots Coupled with Immunomagnetic Separation, PLoS One, vol. 6 (5), pp 1-6, PLoS 2011	en
heal.abstract	Mycobacteria have always proven difficult to identify due to their low growth rate and fastidious nature. Therefore molecular biology and more recently nanotechnology, have been exploited from early on for the detection of these pathogens. Here we present the first stage of development of an assay incorporating cadmium selenide quantum dots (QDs) for the detection of mycobacterial surface antigens. The principle of the assay is the separation of bacterial cells using magnetic beads coupled with genus-specific polyclonal antibodies and monoclonal antibodies for heparin-binding hemagglutinin. These complexes are then tagged with anti-mouse biotinylated antibody and finally streptavidin-conjugated QDs which leads to the detection of a fluorescent signal. For the evaluation of performance, the method under study was applied on Mycobacterium bovis BCG and Mycobacterium tuberculosis (positive controls), as well as E. coli and Salmonella spp. that constituted the negative controls. The direct observation of the latter category of samples did not reveal fluorescence as opposed to the mycobacteria mentioned above. The minimum detection limit of the assay was defined to 104 bacteria/ml, which could be further decreased by a 1 log when fluorescence was measured with a spectrofluorometer. The method described here can be easily adjusted for any other protein target of either the pathogen or the host, and once fully developed it will be directly applicable on clinical samples.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLοS One	en
dc.identifier.doi	10.1371/journal.pone.0020026	en