The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1

dc.contributor.author	Banilas, G	en
dc.contributor.author	Karampelias, M	en
dc.contributor.author	Makariti, I	en
dc.contributor.author	Kourti, A	en
dc.contributor.author	Hatzopoulos, P	en
dc.date.accessioned	2014-06-06T06:51:04Z
dc.date.available	2014-06-06T06:51:04Z
dc.date.issued	2011	en
dc.identifier.issn	00220957	en
dc.identifier.uri	http://dx.doi.org/10.1093/jxb/erq286	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5300
dc.subject	Diacylglycerol acyltransferase	en
dc.subject	gene expression	en
dc.subject	in situ hybridization	en
dc.subject	olive development	en
dc.subject	triacylglycerols	en
dc.subject.other	diacylglycerol acyltransferase	en
dc.subject.other	isoprotein	en
dc.subject.other	vegetable protein	en
dc.subject.other	amino acid sequence	en
dc.subject.other	article	en
dc.subject.other	classification	en
dc.subject.other	enzymology	en
dc.subject.other	gene expression regulation	en
dc.subject.other	genetics	en
dc.subject.other	growth, development and aging	en
dc.subject.other	metabolism	en
dc.subject.other	molecular genetics	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	olive tree	en
dc.subject.other	phylogeny	en
dc.subject.other	Amino Acid Sequence	en
dc.subject.other	Base Sequence	en
dc.subject.other	Diacylglycerol O-Acyltransferase	en
dc.subject.other	Gene Expression Regulation, Developmental	en
dc.subject.other	Gene Expression Regulation, Enzymologic	en
dc.subject.other	Gene Expression Regulation, Plant	en
dc.subject.other	Molecular Sequence Data	en
dc.subject.other	Olea	en
dc.subject.other	Phylogeny	en
dc.subject.other	Plant Proteins	en
dc.subject.other	Protein Isoforms	en
dc.title	The olive DGAT2 gene is developmentally regulated and shares overlapping but distinct expression patterns with DGAT1	en
heal.type	journalArticle	en
heal.identifier.primary	10.1093/jxb/erq286	en
heal.publicationDate	2011	en
heal.abstract	Diacylglycerol acyltransferases (DGATs) catalyse the final step of the triacylglycerol (TAG) biosynthesis of the Kennedy pathway. Two major gene families have been shown to encode DGATs, DGAT1 (type-1) and DGAT2 (type-2). Both genes encode membrane-bound proteins, with no sequence homology to each other. In this study, the molecular cloning and characterization of a type-2 DGAT cDNA from olive is presented. Southern blot analysis showed that OeDGAT2 is represented by a single copy in the olive genome. Comparative transcriptional analysis revealed that DGAT1 and DGAT2 are developmentally regulated and share an overall overlapping but distinct transcription pattern in various tissues during vegetative growth. DGAT2 is highly expressed in mature or senescing olive tissues. In flowers, the expression of DGAT1 was almost undetectable, while DGAT2 transcripts accumulated at the later stages of both anther and ovary development. Differential gene regulation was also detected in the seed and mesocarp, two drupe compartments that largely differ in their functional roles and mode of lipid accumulation. DGAT1 appears to contribute for most of the TAG deposition in seeds, whereas, in the mesocarp, both DGAT1 and DGAT2 share an overlapping expression pattern. During the last stages of mesocarp growth, when TAGs are still accumulating, strong up-regulation of DGAT2 but a marked decline of DGAT1 transcript levels were detected. The present results show overlapping gene expression for olive DGATs during mesocarp growth, with a more prominent implication of DGAT2 in floral bud development and fruit ripening. © 2010 The Author(s).	en
heal.journalName	Journal of Experimental Botany	en
dc.identifier.issue	2	en
dc.identifier.volume	62	en
dc.identifier.doi	10.1093/jxb/erq286	en
dc.identifier.spage	521	en
dc.identifier.epage	532	en