| heal.abstract |
The olive tree (Olea europea L.) is cultivated in the Mediterranean Basin since 5800 BC. Its socio-economic impact is very important for the countries in the area. Greece occupies the third place in the world rank of olive oil producers and the second place in the European Union as a table olive producer. 'Koroneiki' is an olive oil cultivar while 'Kalamon' is a table olive cultivar. Both of them are the most well-known worldwide Greek olive cultivars. In this study, healthy, young leaves of both cultivars were collected from seven different regions in Greece and Cyprus in order to study the intra-varietal variability. DNA extraction was performed according to Doyle and Doyle protocol. Markers originating from two different molecular techniques, Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR), were used for investigating the germplasm variability. In order to establish the genetic relationships among the clones of 'Kalamon' and 'Koroneiki', 30 RAPD primers were tested and 10 were used while for ISSR 10 primers were tested and 6 of them were used. Based on the results from both methods, but primarily from ISSR, intra-varietal variability was present in both cultivars. |
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