Microbial removal of acetate selectively from sugar mixtures

dc.contributor.author	Lakshmanaswamy, A	en
dc.contributor.author	Rajaraman, E	en
dc.contributor.author	Eiteman, MA	en
dc.contributor.author	Altman, E	en
dc.date.accessioned	2014-06-06T06:50:58Z
dc.date.available	2014-06-06T06:50:58Z
dc.date.issued	2011	en
dc.identifier.issn	13675435	en
dc.identifier.uri	http://dx.doi.org/10.1007/s10295-010-0932-1	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5255
dc.subject	Acetate	en
dc.subject	Biomass hydrolysate	en
dc.subject	Ethanol	en
dc.subject	Glucose	en
dc.subject	Xylose	en
dc.subject.other	acetic acid	en
dc.subject.other	glucokinase	en
dc.subject.other	glucose	en
dc.subject.other	xylose	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	crr gene	en
dc.subject.other	Escherichia coli	en
dc.subject.other	gene	en
dc.subject.other	gene mutation	en
dc.subject.other	manZ gene	en
dc.subject.other	nonhuman	en
dc.subject.other	ptsG gene	en
dc.subject.other	sugar intake	en
dc.subject.other	Acetic Acid	en
dc.subject.other	Biomass	en
dc.subject.other	Escherichia coli	en
dc.subject.other	Fermentation	en
dc.subject.other	Glucokinase	en
dc.subject.other	Glucose	en
dc.subject.other	Phosphoenolpyruvate Sugar Phosphotransferase System	en
dc.subject.other	Xylose	en
dc.subject.other	Escherichia coli	en
dc.title	Microbial removal of acetate selectively from sugar mixtures	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s10295-010-0932-1	en
heal.publicationDate	2011	en
heal.abstract	Acetic acid is an unavoidable constituent of the biomass hydrolysates generated from acetylated hemicellulose and lignin, and acetate affects the performance of microbes used to convert these hydrolysates into biofuels or other biochemicals. In this study, acetate was selectively removed from synthetic mixtures of glucose and xylose using metabolically engineered Escherichia coli strains having mutations in the glucose phosphotransferase system (PTS) genes (ptsG, manZ, crr), glucokinase (glk), and xylose (xylA). In batch culture, ALS1060 (ptsG manZ glk xylA) consumed exclusively acetate to depletion, and then consumed the two sugars only at a very slow rate (a growth rate of about 0.01 h -1). We also examined the effects of an additional knockout of either malX, fruA, fruB, bglF, or crr, genes that are involved in other PTSs, and a batch process using KD840 (ptsG manZ glk crr xylA) demonstrated a further reduction in glucose or xylose consumption by E. coli. These results demonstrate the feasibility of using a substrate-selective approach for the pre-treatment of biomass hydrolysate for microbial processes. © 2011 Society for Industrial Microbiology.	en
heal.journalName	Journal of Industrial Microbiology and Biotechnology	en
dc.identifier.issue	9	en
dc.identifier.volume	38	en
dc.identifier.doi	10.1007/s10295-010-0932-1	en
dc.identifier.spage	1477	en
dc.identifier.epage	1484	en