Effects of grape extracts on the in vitro activity of enzymes involved in oxidative stress regulation

dc.contributor.author	Chrysoula, S	en
dc.contributor.author	Veskoukis, AS	en
dc.contributor.author	Dimitrios, S	en
dc.contributor.author	Kalliopi, L	en
dc.contributor.author	Maria, A	en
dc.contributor.author	Haroutounian, SA	en
dc.contributor.author	Maria, T	en
dc.contributor.author	Eleni, T	en
dc.contributor.author	Dimitrios, K	en
dc.date.accessioned	2014-06-06T06:50:55Z
dc.date.available	2014-06-06T06:50:55Z
dc.date.issued	2011	en
dc.identifier.issn	0258851X	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5224
dc.relation.uri	http://www.scopus.com/inward/record.url?eid=2-s2.0-79960621376&partnerID=40&md5=eefa1b68534ed78e04d906962202ac5b	en
dc.subject	Enzymes	en
dc.subject	Food supplements	en
dc.subject	Grapes	en
dc.subject	Oxidative stress	en
dc.subject	Plant extracts	en
dc.subject.other	catalase	en
dc.subject.other	polyphenol	en
dc.subject.other	superoxide dismutase	en
dc.subject.other	Vitis vinifera extract	en
dc.subject.other	xanthine oxidase	en
dc.subject.other	antioxidant activity	en
dc.subject.other	article	en
dc.subject.other	berry	en
dc.subject.other	enzyme induction	en
dc.subject.other	enzyme inhibition	en
dc.subject.other	human	en
dc.subject.other	human experiment	en
dc.subject.other	in vitro study	en
dc.subject.other	male	en
dc.subject.other	nonhuman	en
dc.subject.other	oxidative stress	en
dc.subject.other	plant stem	en
dc.subject.other	Adult	en
dc.subject.other	Antioxidants	en
dc.subject.other	Catalase	en
dc.subject.other	Enzyme Activation	en
dc.subject.other	Humans	en
dc.subject.other	Male	en
dc.subject.other	Oxidative Stress	en
dc.subject.other	Oxidoreductases	en
dc.subject.other	Plant Extracts	en
dc.subject.other	Superoxide Dismutase	en
dc.subject.other	Vitis	en
dc.subject.other	Xanthine Oxidase	en
dc.title	Effects of grape extracts on the in vitro activity of enzymes involved in oxidative stress regulation	en
heal.type	journalArticle	en
heal.publicationDate	2011	en
heal.abstract	Background/Aim: A number of in vitro and in vivo studies have investigated the potential preventive activity of grape extracts against different diseases, and have mostly focused on their antioxidant properties. The present study examined the effects of 21 extracts from stem, skin and berry from Greek grape varieties on the activity of enzymes involved in regulation of oxidative stress, namely xanthine oxidase (XO), catalase (CAT) and superoxide dismutase (SOD). Materials and Methods: The effects of the extracts on the enzymatic activity of XO, CAT and SOD were studied spectrophotometrically. Results: The tested extracts inhibited CAT and XO activity, while higher extract concentrations are required to induce SOD. However, stem and skin extracts exhibited a different inhibitory pattern against CAT and XO compared to berry extracts. The observed differences are possibly attributed to the extract polyphenolic composition. Conclusion: Although the induction of SOD activity suggests an antioxidant capacity, the inhibition of CAT and XO indicates a pro-oxidant action. In general, the extracts showed pro-oxidant activity, possibly dependent on both their polyphenolic composition and concentration.	en
heal.journalName	In Vivo	en
dc.identifier.issue	4	en
dc.identifier.volume	25	en
dc.identifier.spage	657	en
dc.identifier.epage	662	en