Differential screening of the neurotoxicity of insecticides by means of a novel electrophysiological biosensor

dc.contributor.author	Voumvouraki, E	en
dc.contributor.author	Kintzios, S	en
dc.date.accessioned	2014-06-06T06:50:53Z
dc.date.available	2014-06-06T06:50:53Z
dc.date.issued	2011	en
dc.identifier.issn	18777058	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.proeng.2011.12.237	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5212
dc.subject	BERA	en
dc.subject	Biosensor	en
dc.subject	Insecticides	en
dc.subject	Neurotoxicity	en
dc.subject.other	Abamectin	en
dc.subject.other	Avermectins	en
dc.subject.other	BERA	en
dc.subject.other	Bioelectric recognition assays	en
dc.subject.other	Calcium alginate gels	en
dc.subject.other	Cell-based	en
dc.subject.other	Chemical substance	en
dc.subject.other	Conventional methods	en
dc.subject.other	Cypermethrins	en
dc.subject.other	Cytotoxic activities	en
dc.subject.other	Differential screening	en
dc.subject.other	Electrode material	en
dc.subject.other	N2a cells	en
dc.subject.other	Neuroblastomas	en
dc.subject.other	Neurotoxicity	en
dc.subject.other	Operational parameters	en
dc.subject.other	Optimal configurations	en
dc.subject.other	Pesticide residue	en
dc.subject.other	Physiological functions	en
dc.subject.other	Pyrethroid insecticide	en
dc.subject.other	Electrophysiology	en
dc.subject.other	Insect control	en
dc.subject.other	Insecticides	en
dc.subject.other	Organic chemicals	en
dc.subject.other	Veterinary medicine	en
dc.subject.other	Biosensors	en
dc.title	Differential screening of the neurotoxicity of insecticides by means of a novel electrophysiological biosensor	en
heal.type	conferenceItem	en
heal.identifier.primary	10.1016/j.proeng.2011.12.237	en
heal.publicationDate	2011	en
heal.abstract	More than 500 different organic chemical substances are used worldwide as pesticides in agriculture and veterinary science. There is increasing evidence that many of these compounds cause moderate to severe neurological damage, due to cytotoxic activity on the foetus or the adult. Conventional methods for assessing the neurotoxicity of pesticides and their residues are very elaborate, time-consuming and expensive. For this reason, a novel cell-based biosensory method has been developed, for the detection of pesticide residues based on the Bioelectric Recognition Assay, (BERA). Neuroblastoma N2a cells have been used, immobilized in calcium alginate gel in order to preserve their physiological functions. The avermectin abamectin was chosen as a model insecticide. Various operational parameters were investigated, such as assay temperature and electrode material. In its optimal configuration, the novel biosensor responded to different abamectin concentrations in a linear fashion, with satisfactory sensitivity (10 ppb) and at a considerably high speed (100-180 sec). The selectivity of the assay against a pyrethroid insecticide, αcypermethrin, was also validated. © 2011 Published by Elsevier Ltd.	en
heal.journalName	Procedia Engineering	en
dc.identifier.volume	25	en
dc.identifier.doi	10.1016/j.proeng.2011.12.237	en
dc.identifier.spage	964	en
dc.identifier.epage	967	en