| dc.contributor.author |
Guan, Y |
en |
| dc.contributor.author |
Kisaalita, W |
en |
| dc.date.accessioned |
2014-06-06T06:50:52Z |
|
| dc.date.available |
2014-06-06T06:50:52Z |
|
| dc.date.issued |
2011 |
en |
| dc.identifier.issn |
09277765 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.colsurfb.2010.12.007 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5198 |
|
| dc.subject |
Cell adhesion |
en |
| dc.subject |
Cell locomotion |
en |
| dc.subject |
Image processing |
en |
| dc.subject |
Laser ablation |
en |
| dc.subject |
Time-lapse microscopy |
en |
| dc.subject.other |
Ablation process |
en |
| dc.subject.other |
Anchorage-dependent cells |
en |
| dc.subject.other |
ArF excimer laser |
en |
| dc.subject.other |
Cell locomotion |
en |
| dc.subject.other |
Edge effect |
en |
| dc.subject.other |
Fibroblast cells |
en |
| dc.subject.other |
Glass controls |
en |
| dc.subject.other |
Glass substrates |
en |
| dc.subject.other |
Human fibroblast cells |
en |
| dc.subject.other |
Implantable devices |
en |
| dc.subject.other |
Laser fluences |
en |
| dc.subject.other |
Life span |
en |
| dc.subject.other |
Micro structuring |
en |
| dc.subject.other |
Micro wells |
en |
| dc.subject.other |
Microstructured materials |
en |
| dc.subject.other |
Precise control |
en |
| dc.subject.other |
Pulse number |
en |
| dc.subject.other |
Repetition rate |
en |
| dc.subject.other |
Time-lapse microscopy |
en |
| dc.subject.other |
Aspect ratio |
en |
| dc.subject.other |
Cell adhesion |
en |
| dc.subject.other |
Cell culture |
en |
| dc.subject.other |
Excimer lasers |
en |
| dc.subject.other |
Fibroblasts |
en |
| dc.subject.other |
Gas lasers |
en |
| dc.subject.other |
Glass |
en |
| dc.subject.other |
Glass lasers |
en |
| dc.subject.other |
Image processing |
en |
| dc.subject.other |
Imaging systems |
en |
| dc.subject.other |
Implants (surgical) |
en |
| dc.subject.other |
Laser ablation |
en |
| dc.subject.other |
Laser applications |
en |
| dc.subject.other |
Microchannels |
en |
| dc.subject.other |
Pulse repetition rate |
en |
| dc.subject.other |
Substrates |
en |
| dc.subject.other |
Adhesion |
en |
| dc.subject.other |
glass |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cell adhesion |
en |
| dc.subject.other |
cell anchorage |
en |
| dc.subject.other |
cell contact |
en |
| dc.subject.other |
cell interaction |
en |
| dc.subject.other |
cell kinetics |
en |
| dc.subject.other |
cell migration |
en |
| dc.subject.other |
cell spreading |
en |
| dc.subject.other |
cell surface |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
fibroblast culture |
en |
| dc.subject.other |
human |
en |
| dc.subject.other |
human cell |
en |
| dc.subject.other |
image processing |
en |
| dc.subject.other |
microanalysis |
en |
| dc.subject.other |
microscope image |
en |
| dc.subject.other |
microtechnology |
en |
| dc.subject.other |
priority journal |
en |
| dc.subject.other |
process optimization |
en |
| dc.subject.other |
structure analysis |
en |
| dc.subject.other |
surface property |
en |
| dc.subject.other |
time lapse imaging |
en |
| dc.subject.other |
Cell Adhesion |
en |
| dc.subject.other |
Cell Movement |
en |
| dc.subject.other |
Cells, Cultured |
en |
| dc.subject.other |
Fibroblasts |
en |
| dc.subject.other |
Glass |
en |
| dc.subject.other |
Humans |
en |
| dc.subject.other |
Surface Properties |
en |
| dc.title |
Cell adhesion and locomotion on microwell-structured glass substrates |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.colsurfb.2010.12.007 |
en |
| heal.publicationDate |
2011 |
en |
| heal.abstract |
The purpose of this study was to investigate the effect of microstructured material surface on cell adhesion and locomotion in real-time. ArF excimer laser direct-writing ablation was used to fabricate microwell patterns with precise control of size and spacing on glass. The influence of the ablation process parameters (laser fluence, pulse number and repetition rate) on the micromachining quality (depth, width, aspect ratio and edge effects) of the microwells was established. Human fibroblast cells, as an example of anchorage-dependent cells, were seeded onto the microstructured glass substrate and time-lapse microscopy was used to study cell adhesion and locomotion. The interaction with microstructured materials resulted in fibroblast cell repulsion and the cells exhibited a higher locomotion speed (75.77 ± 3.36 μm/h) on the structures in comparison with plane glass control (54.01 ± 15.53 μm/h). Further studies are needed to firmly establish the potential of microstructuring, for example, in elongating the life spans of implantable devices. © 2010 Elsevier B.V. |
en |
| heal.journalName |
Colloids and Surfaces B: Biointerfaces |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
84 |
en |
| dc.identifier.doi |
10.1016/j.colsurfb.2010.12.007 |
en |
| dc.identifier.spage |
35 |
en |
| dc.identifier.epage |
43 |
en |