| dc.contributor.author |
Valero, T |
en |
| dc.contributor.author |
Moschopoulou, G |
en |
| dc.contributor.author |
Kintzios, S |
en |
| dc.contributor.author |
Hauptmann, P |
en |
| dc.contributor.author |
Naumann, M |
en |
| dc.contributor.author |
Jacobs, T |
en |
| dc.date.accessioned |
2014-06-06T06:50:44Z |
|
| dc.date.available |
2014-06-06T06:50:44Z |
|
| dc.date.issued |
2010 |
en |
| dc.identifier.issn |
09565663 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.bios.2010.07.066 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5136 |
|
| dc.subject |
Collagen |
en |
| dc.subject |
Electrical impedance spectroscopy |
en |
| dc.subject |
Impedimetric biosensor |
en |
| dc.subject |
Laminin |
en |
| dc.subject |
Neural differentiation |
en |
| dc.subject |
Three-dimensional cell culture |
en |
| dc.subject.other |
Cell number |
en |
| dc.subject.other |
Cellular differentiation |
en |
| dc.subject.other |
Cellular model |
en |
| dc.subject.other |
Central nervous systems |
en |
| dc.subject.other |
Conductive properties |
en |
| dc.subject.other |
Differentiation process |
en |
| dc.subject.other |
Drug screening |
en |
| dc.subject.other |
Electrical impedance spectroscopy |
en |
| dc.subject.other |
Gel matrix |
en |
| dc.subject.other |
Gel mixture |
en |
| dc.subject.other |
Immobilization process |
en |
| dc.subject.other |
Laminin |
en |
| dc.subject.other |
Neural cells |
en |
| dc.subject.other |
Neural differentiations |
en |
| dc.subject.other |
Neuroblastoma cells |
en |
| dc.subject.other |
Neuronal cell |
en |
| dc.subject.other |
Neuronal differentiation |
en |
| dc.subject.other |
On-line monitors |
en |
| dc.subject.other |
Regenerative medicine |
en |
| dc.subject.other |
Serum-free medium |
en |
| dc.subject.other |
Specific resistivities |
en |
| dc.subject.other |
Three dimensional cell culture |
en |
| dc.subject.other |
Three-dimensional model |
en |
| dc.subject.other |
Biosensors |
en |
| dc.subject.other |
Collagen |
en |
| dc.subject.other |
Electric impedance |
en |
| dc.subject.other |
Electric impedance measurement |
en |
| dc.subject.other |
Gels |
en |
| dc.subject.other |
Three dimensional |
en |
| dc.subject.other |
Cell culture |
en |
| dc.subject.other |
collagen |
en |
| dc.subject.other |
laminin |
en |
| dc.subject.other |
animal cell |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
biosensor |
en |
| dc.subject.other |
cell count |
en |
| dc.subject.other |
cell culture |
en |
| dc.subject.other |
cell differentiation |
en |
| dc.subject.other |
controlled study |
en |
| dc.subject.other |
dielectric constant |
en |
| dc.subject.other |
drug screening |
en |
| dc.subject.other |
gel |
en |
| dc.subject.other |
immobilization |
en |
| dc.subject.other |
impedance |
en |
| dc.subject.other |
nerve cell |
en |
| dc.subject.other |
neurobiology |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
three dimensional imaging |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Biosensing Techniques |
en |
| dc.subject.other |
Cell Count |
en |
| dc.subject.other |
Cell Differentiation |
en |
| dc.subject.other |
Cell Line |
en |
| dc.subject.other |
Cell Survival |
en |
| dc.subject.other |
Cells, Immobilized |
en |
| dc.subject.other |
Collagen |
en |
| dc.subject.other |
Culture Media, Serum-Free |
en |
| dc.subject.other |
Dielectric Spectroscopy |
en |
| dc.subject.other |
Laminin |
en |
| dc.subject.other |
Mice |
en |
| dc.subject.other |
Neurons |
en |
| dc.subject.other |
Signal Transduction |
en |
| dc.title |
Studies on neuronal differentiation and signalling processes with a novel impedimetric biosensor |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.bios.2010.07.066 |
en |
| heal.publicationDate |
2010 |
en |
| heal.abstract |
The differentiation of neural cells is an important process during the development of the central nervous system. Studies on the mechanisms of the differentiation process is of special importance, e.g. in the field of regenerative medicine. In this contribution the cellular differentiation of gel matrix embedded neuronal cells was studied. The three-dimensional organization of neuronal cells represents a new cellular model system closer to the physiology than conventional two-dimensional cell cultures. Neuro2a (N2a) neuroblastoma cells were immobilized in different gel matrices and the grade of differentiation was compared. Furthermore, the impact of the cell number and selected differentiation factors were analyzed. Experimental results revealed that gel matrices based on collagen-laminin mixtures in contact with serum free medium enable neural differentiation. Therefore, collagen-laminin gels appear as a suitable three-dimensional model for drug screening in developmental neurobiology. Following optimization of the immobilization process, a novel impedimetric sensor and electrical impedance spectroscopy technique was applied to on-line monitor the differentiation process by means of changes in the dielectric and conductive properties. Experimental results showed an increase in the impedance magnitude that can be mainly attributed to differentiating cells accompanied by an increase in the specific resistivity of the bare gel mixture. © 2010 Elsevier B.V. |
en |
| heal.journalName |
Biosensors and Bioelectronics |
en |
| dc.identifier.issue |
4 |
en |
| dc.identifier.volume |
26 |
en |
| dc.identifier.doi |
10.1016/j.bios.2010.07.066 |
en |
| dc.identifier.spage |
1407 |
en |
| dc.identifier.epage |
1413 |
en |