| dc.contributor.author |
Labrou, NE |
en |
| dc.date.accessioned |
2014-06-06T06:50:40Z |
|
| dc.date.available |
2014-06-06T06:50:40Z |
|
| dc.date.issued |
2010 |
en |
| dc.identifier.issn |
13892037 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.2174/138920310790274617 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5110 |
|
| dc.subject.other |
chemical mutagen |
en |
| dc.subject.other |
DNA |
en |
| dc.subject.other |
enzyme |
en |
| dc.subject.other |
analytical error |
en |
| dc.subject.other |
chemical mutagenesis |
en |
| dc.subject.other |
combinatorial library |
en |
| dc.subject.other |
directed molecular evolution |
en |
| dc.subject.other |
DNA replication |
en |
| dc.subject.other |
DNA sequence |
en |
| dc.subject.other |
enzyme activity |
en |
| dc.subject.other |
enzyme structure |
en |
| dc.subject.other |
Escherichia coli |
en |
| dc.subject.other |
gene amplification |
en |
| dc.subject.other |
genetic strain |
en |
| dc.subject.other |
genetic variability |
en |
| dc.subject.other |
in vitro study |
en |
| dc.subject.other |
molecular cloning |
en |
| dc.subject.other |
mutagenesis |
en |
| dc.subject.other |
mutator gene |
en |
| dc.subject.other |
nonhuman |
en |
| dc.subject.other |
polymerase chain reaction |
en |
| dc.subject.other |
review |
en |
| dc.subject.other |
rolling circle amplification |
en |
| dc.subject.other |
saturation mutagenesis |
en |
| dc.subject.other |
site directed mutagenesis |
en |
| dc.subject.other |
site saturationn mutagenesis |
en |
| dc.subject.other |
Directed Molecular Evolution |
en |
| dc.subject.other |
Enzymes |
en |
| dc.subject.other |
Gene Library |
en |
| dc.subject.other |
Humans |
en |
| dc.subject.other |
Mutagenesis |
en |
| dc.title |
Random mutagenesis methods for in vitro directed enzyme evolution |
en |
| heal.type |
other |
en |
| heal.identifier.primary |
10.2174/138920310790274617 |
en |
| heal.publicationDate |
2010 |
en |
| heal.abstract |
Random mutagenesis is a powerful tool for generating enzymes, proteins, entire metabolic pathways, or even entire genomes with desired or improved properties. This technology is used to evolve genes in vitro through an iterative process consisting of recombinant generation. Coupled with the development of powerful high-throughput screening or selection methods, this technique has been successfully used to solve problems in protein engineering. There are many methods to generate genetic diversity by random mutagenesis and to create combinatorial libraries. This can be achieved by treating DNA or whole bacteria with various chemical mutagens, by passing cloned genes through mutator strains, by ""error-prone"" PCR mutagenesis, by rolling circle error-prone PCR, or by saturation mutagenesis. The next sections of this review article focus on recent advances in techniques and methods used for in vitro directed evolution of enzymes using random mutagenesis. Selected examples, highlighting successful applications of these methods, are also presented and discussed. © 2010 Bentham Science Publishers Ltd. |
en |
| heal.journalName |
Current Protein and Peptide Science |
en |
| dc.identifier.issue |
1 |
en |
| dc.identifier.volume |
11 |
en |
| dc.identifier.doi |
10.2174/138920310790274617 |
en |
| dc.identifier.spage |
91 |
en |
| dc.identifier.epage |
100 |
en |