Random mutagenesis methods for in vitro directed enzyme evolution

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dc.contributor.author Labrou, NE en
dc.date.accessioned 2014-06-06T06:50:40Z
dc.date.available 2014-06-06T06:50:40Z
dc.date.issued 2010 en
dc.identifier.issn 13892037 en
dc.identifier.uri http://dx.doi.org/10.2174/138920310790274617 en
dc.subject.other chemical mutagen en
dc.subject.other DNA en
dc.subject.other enzyme en
dc.subject.other analytical error en
dc.subject.other chemical mutagenesis en
dc.subject.other combinatorial library en
dc.subject.other directed molecular evolution en
dc.subject.other DNA replication en
dc.subject.other DNA sequence en
dc.subject.other enzyme activity en
dc.subject.other enzyme structure en
dc.subject.other Escherichia coli en
dc.subject.other gene amplification en
dc.subject.other genetic strain en
dc.subject.other genetic variability en
dc.subject.other in vitro study en
dc.subject.other molecular cloning en
dc.subject.other mutagenesis en
dc.subject.other mutator gene en
dc.subject.other nonhuman en
dc.subject.other polymerase chain reaction en
dc.subject.other review en
dc.subject.other rolling circle amplification en
dc.subject.other saturation mutagenesis en
dc.subject.other site directed mutagenesis en
dc.subject.other site saturationn mutagenesis en
dc.subject.other Directed Molecular Evolution en
dc.subject.other Enzymes en
dc.subject.other Gene Library en
dc.subject.other Humans en
dc.subject.other Mutagenesis en
dc.title Random mutagenesis methods for in vitro directed enzyme evolution en
heal.type other en
heal.identifier.primary 10.2174/138920310790274617 en
heal.publicationDate 2010 en
heal.abstract Random mutagenesis is a powerful tool for generating enzymes, proteins, entire metabolic pathways, or even entire genomes with desired or improved properties. This technology is used to evolve genes in vitro through an iterative process consisting of recombinant generation. Coupled with the development of powerful high-throughput screening or selection methods, this technique has been successfully used to solve problems in protein engineering. There are many methods to generate genetic diversity by random mutagenesis and to create combinatorial libraries. This can be achieved by treating DNA or whole bacteria with various chemical mutagens, by passing cloned genes through mutator strains, by ""error-prone"" PCR mutagenesis, by rolling circle error-prone PCR, or by saturation mutagenesis. The next sections of this review article focus on recent advances in techniques and methods used for in vitro directed evolution of enzymes using random mutagenesis. Selected examples, highlighting successful applications of these methods, are also presented and discussed. © 2010 Bentham Science Publishers Ltd. en
heal.journalName Current Protein and Peptide Science en
dc.identifier.issue 1 en
dc.identifier.volume 11 en
dc.identifier.doi 10.2174/138920310790274617 en
dc.identifier.spage 91 en
dc.identifier.epage 100 en

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