Overexpression of a specific soybean GmGSTU4 isoenzyme improves diphenyl ether and chloroacetanilide herbicide tolerance of transgenic tobacco plants

dc.contributor.author	Benekos, K	en
dc.contributor.author	Kissoudis, C	en
dc.contributor.author	Nianiou-Obeidat, I	en
dc.contributor.author	Labrou, N	en
dc.contributor.author	Madesis, P	en
dc.contributor.author	Kalamaki, M	en
dc.contributor.author	Makris, A	en
dc.contributor.author	Tsaftaris, A	en
dc.date.accessioned	2014-06-06T06:50:37Z
dc.date.available	2014-06-06T06:50:37Z
dc.date.issued	2010	en
dc.identifier.issn	01681656	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.jbiotec.2010.07.011	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5087
dc.subject	Chloroacetanilide	en
dc.subject	Diphenyl ether	en
dc.subject	GST	en
dc.subject	Herbicide detoxification	en
dc.subject	Tobacco	en
dc.subject.other	Agrobacterium	en
dc.subject.other	Alachlor	en
dc.subject.other	Catalytic activity	en
dc.subject.other	Chloroacetanilide herbicides	en
dc.subject.other	Chloroacetanilides	en
dc.subject.other	Cumene hydroperoxide	en
dc.subject.other	Diphenyl ether	en
dc.subject.other	Electrolyte leakage	en
dc.subject.other	Environmental cleanup	en
dc.subject.other	Glutathione transferase	en
dc.subject.other	Glutathiones	en
dc.subject.other	GST	en
dc.subject.other	Herbicide detoxification	en
dc.subject.other	In-vitro	en
dc.subject.other	Leaf extracts	en
dc.subject.other	Metolachlors	en
dc.subject.other	Over-expression	en
dc.subject.other	Phytoremediation	en
dc.subject.other	Southern blot hybridization	en
dc.subject.other	Transgenic plants	en
dc.subject.other	Transgenic tobacco plants	en
dc.subject.other	Wild-type plants	en
dc.subject.other	Bioremediation	en
dc.subject.other	Catalyst activity	en
dc.subject.other	Detoxification	en
dc.subject.other	Enzymes	en
dc.subject.other	Ethers	en
dc.subject.other	Organic compounds	en
dc.subject.other	Plants (botany)	en
dc.subject.other	Tobacco	en
dc.subject.other	Weed control	en
dc.subject.other	Herbicides	en
dc.subject.other	alachlor	en
dc.subject.other	anilide	en
dc.subject.other	chloroacetanilide	en
dc.subject.other	cumene hydroperoxide	en
dc.subject.other	diphenyl ether	en
dc.subject.other	glutathione dependent peroxidase	en
dc.subject.other	glutathione transferase	en
dc.subject.other	glutathione transferase u4	en
dc.subject.other	herbicide	en
dc.subject.other	isoenzyme	en
dc.subject.other	metolachlor	en
dc.subject.other	peroxidase	en
dc.subject.other	plant extract	en
dc.subject.other	unclassified drug	en
dc.subject.other	article	en
dc.subject.other	cultivar	en
dc.subject.other	detoxification	en
dc.subject.other	enzyme activity	en
dc.subject.other	gene overexpression	en
dc.subject.other	nonhuman	en
dc.subject.other	phenotype	en
dc.subject.other	phytoremediation	en
dc.subject.other	plant growth	en
dc.subject.other	priority journal	en
dc.subject.other	reverse transcription polymerase chain reaction	en
dc.subject.other	Rhizobium	en
dc.subject.other	Southern blotting	en
dc.subject.other	soybean	en
dc.subject.other	tobacco	en
dc.subject.other	Acetamides	en
dc.subject.other	Glutathione Transferase	en
dc.subject.other	Isoenzymes	en
dc.subject.other	Metabolic Detoxication, Drug	en
dc.subject.other	Phenotype	en
dc.subject.other	Phenyl Ethers	en
dc.subject.other	Plant Leaves	en
dc.subject.other	Plants, Genetically Modified	en
dc.subject.other	Soybean Proteins	en
dc.subject.other	Tobacco	en
dc.subject.other	Agrobacterium	en
dc.subject.other	Glycine max	en
dc.subject.other	Nicotiana obtusifolia	en
dc.subject.other	Nicotiana tabacum	en
dc.title	Overexpression of a specific soybean GmGSTU4 isoenzyme improves diphenyl ether and chloroacetanilide herbicide tolerance of transgenic tobacco plants	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.jbiotec.2010.07.011	en
heal.publicationDate	2010	en
heal.abstract	Plant glutathione transferases (GSTs) superfamily consists of multifunctional enzymes and forms a major part of the plants herbicide detoxification enzyme network. The tau class GST isoenzyme GmGSTU4 from soybean, exhibits catalytic activity towards the diphenyl ether herbicide fluorodifen and is active as glutathione-dependent peroxidase (GPOX). Transgenic tobacco plants of Basmas cultivar were generated via Agrobacterium transformation. The aim was to evaluate in planta, GmGSTU4's role in detoxifying the diphenyl ether herbicides fluorodifen and oxyfluorfen and the chloroacetanilides alachlor and metolachlor. Transgenic tobacco plants were verified by PCR and Southern blot hybridization and expression of GmGSTU4 was determined by RT-PCR. Leaf extracts from transgenic plants showed moderate increase in GST activity towards CDNB and a significant increase towards fluorodifen and alachlor, and at the same time an increased GPOX activity towards cumene hydroperoxide. GmGSTU4 overexpressing plants when treated with 200 μM fluorodifen or oxyfluorfen exhibited reduced relative electrolyte leakage compared to wild type plants. Moreover all GmGSTU4 overexpressing lines exhibited significantly increased tolerance towards alachlor when grown in vitro at 7.5. mg/L alachlor compared to wild type plants. No significant increased tolerance was observed to metolachlor. These results confirm the contribution of this particular GmGSTU4 isoenzyme from soybean in the detoxification of fluorodifen and alachlor, and provide the basis towards the development of transgenic plants with improved phytoremediation capabilities for future use in environmental cleanup of herbicides. © 2010 Elsevier B.V.	en
heal.journalName	Journal of Biotechnology	en
dc.identifier.issue	1	en
dc.identifier.volume	150	en
dc.identifier.doi	10.1016/j.jbiotec.2010.07.011	en
dc.identifier.spage	195	en
dc.identifier.epage	201	en