| dc.contributor.author |
Xu, Y |
en |
| dc.contributor.author |
Wang, R-H |
en |
| dc.contributor.author |
Koutinas, AA |
en |
| dc.contributor.author |
Webb, C |
en |
| dc.date.accessioned |
2014-06-06T06:50:33Z |
|
| dc.date.available |
2014-06-06T06:50:33Z |
|
| dc.date.issued |
2010 |
en |
| dc.identifier.issn |
13595113 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.procbio.2009.09.001 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/5069 |
|
| dc.subject |
Poly(3-hydroxybutyrate) |
en |
| dc.subject |
Wautersia eutropha |
en |
| dc.subject |
Wheat-based biorefinery |
en |
| dc.subject.other |
Bacterial cells |
en |
| dc.subject.other |
Biodegradable plastics |
en |
| dc.subject.other |
Biorefining |
en |
| dc.subject.other |
Casein hydrolysate |
en |
| dc.subject.other |
Commercial sources |
en |
| dc.subject.other |
Commercialisation |
en |
| dc.subject.other |
Conversion yield |
en |
| dc.subject.other |
Corn steep liquor |
en |
| dc.subject.other |
Downstream-processing |
en |
| dc.subject.other |
Dry weight |
en |
| dc.subject.other |
Environmentally benign |
en |
| dc.subject.other |
Fed batches |
en |
| dc.subject.other |
PHB production |
en |
| dc.subject.other |
Poly(3-hydroxybutyrate) |
en |
| dc.subject.other |
Polyhydroxyalkanoates |
en |
| dc.subject.other |
Transmission electron microscope |
en |
| dc.subject.other |
Wautersia eutropha |
en |
| dc.subject.other |
Wheat gluten |
en |
| dc.subject.other |
Wheat-based biorefinery |
en |
| dc.subject.other |
Yeast extracts |
en |
| dc.subject.other |
Amines |
en |
| dc.subject.other |
Amino acids |
en |
| dc.subject.other |
Antigen-antibody reactions |
en |
| dc.subject.other |
Bacteriology |
en |
| dc.subject.other |
Biodegradable polymers |
en |
| dc.subject.other |
Electron microscopes |
en |
| dc.subject.other |
Fermentation |
en |
| dc.subject.other |
Glucose |
en |
| dc.subject.other |
Inorganic chemicals |
en |
| dc.subject.other |
Inorganic compounds |
en |
| dc.subject.other |
Organic acids |
en |
| dc.subject.other |
Plastics |
en |
| dc.subject.other |
Proteins |
en |
| dc.subject.other |
Purification |
en |
| dc.subject.other |
Transmission electron microscopy |
en |
| dc.subject.other |
Bioconversion |
en |
| dc.subject.other |
Bacteria (microorganisms) |
en |
| dc.subject.other |
Cupriavidus necator |
en |
| dc.subject.other |
Glycine max |
en |
| dc.subject.other |
Triticum aestivum |
en |
| dc.subject.other |
Zea mays |
en |
| dc.title |
Microbial biodegradable plastic production from a wheat-based biorefining strategy |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.procbio.2009.09.001 |
en |
| heal.publicationDate |
2010 |
en |
| heal.abstract |
Restructuring the current fermentation and recovery practices employed for the production of polyhydroxyalkanoates is essential for the commercialisation of environmentally benign and cost competitive biodegradable plastics. This study presents the potential of a wheat-based biorefinery for the production of poly(3-hydroxybutyrate) (PHB). Fed-batch bioconversions using Wautersia eutropha growing on wheat-derived media led to the production of 162.8 g/l PHB. A high PHB to total dry weight (TDW) yield of 93% (w/w) was achieved due to microbial autolysis at the end of fermentation. Images of bacterial cells taken with a Transmission Electron Microscope (TEM) indicated the potential of bacterial autolysis as a mean to shorten downstream processing for PHB purification. The consumption of amino acids and peptides derived from wheat gluten hydrolysis resulted in a high glucose to PHB conversion yield of 0.47 g/g. The respective yield regarding the amount of wheat used for the production of enzymes and PHB was around 0.3 g PHB/g wheat, which corresponds to 82.8% of the maximum theoretical conversion yield. The productivity achieved was around 0.9 g/l h. Fermentations carried out on wheat-derived media and media formulated with various commercial sources of nutrients (glucose, yeast extract, soy-protein acid hydrolysate, casein hydrolysates, corn steep liquor and various inorganic chemicals) showed that the proposed wheat-based biorefinery strategy enhanced PHB production. © 2009 Elsevier Ltd. All rights reserved. |
en |
| heal.journalName |
Process Biochemistry |
en |
| dc.identifier.issue |
2 |
en |
| dc.identifier.volume |
45 |
en |
| dc.identifier.doi |
10.1016/j.procbio.2009.09.001 |
en |
| dc.identifier.spage |
153 |
en |
| dc.identifier.epage |
163 |
en |