Lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection

dc.contributor.author	Maragkoudakis, PA	en
dc.contributor.author	Chingwaru, W	en
dc.contributor.author	Gradisnik, L	en
dc.contributor.author	Tsakalidou, E	en
dc.contributor.author	Cencic, A	en
dc.date.accessioned	2014-06-06T06:50:33Z
dc.date.available	2014-06-06T06:50:33Z
dc.date.issued	2010	en
dc.identifier.issn	01681605	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.ijfoodmicro.2009.12.024	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5064
dc.subject	Attachment	en
dc.subject	Cell lines	en
dc.subject	Probiotic	en
dc.subject	Reactive oxygen species	en
dc.subject	Rotavirus	en
dc.subject	Transmissible gastroenteritis virus	en
dc.subject.other	aca dc 146	en
dc.subject.other	aca dc 179	en
dc.subject.other	aca dc 4037	en
dc.subject.other	bfe 2207	en
dc.subject.other	bfe 5092	en
dc.subject.other	bfe 900	en
dc.subject.other	hydrogen peroxide	en
dc.subject.other	lcs	en
dc.subject.other	lgg	en
dc.subject.other	nitric oxide	en
dc.subject.other	pca 142	en
dc.subject.other	pca 185	en
dc.subject.other	pca 227	en
dc.subject.other	pca 236	en
dc.subject.other	pcd 71	en
dc.subject.other	pck 38	en
dc.subject.other	pcs 20	en
dc.subject.other	pcs 22	en
dc.subject.other	pcs 25	en
dc.subject.other	pcs 26	en
dc.subject.other	probiotic agent	en
dc.subject.other	reactive oxygen metabolite	en
dc.subject.other	unclassified drug	en
dc.subject.other	antiviral activity	en
dc.subject.other	article	en
dc.subject.other	bacterial strain	en
dc.subject.other	cell line	en
dc.subject.other	enteric virus	en
dc.subject.other	Enterococcus faecium	en
dc.subject.other	human	en
dc.subject.other	immunocompetent cell	en
dc.subject.other	intestine epithelium cell	en
dc.subject.other	intestine infection	en
dc.subject.other	lactic acid bacterium	en
dc.subject.other	Lactobacillus casei	en
dc.subject.other	Lactobacillus fermentum	en
dc.subject.other	Lactobacillus gasseri	en
dc.subject.other	Lactobacillus paracasei	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	Lactobacillus rhamnosus	en
dc.subject.other	macrophage	en
dc.subject.other	monolayer culture	en
dc.subject.other	nonhuman	en
dc.subject.other	protection	en
dc.subject.other	Rotavirus	en
dc.subject.other	Transmissible gastroenteritis virus	en
dc.subject.other	virus infection	en
dc.subject.other	Animals	en
dc.subject.other	Bacterial Adhesion	en
dc.subject.other	Enterococcus	en
dc.subject.other	Enterovirus Infections	en
dc.subject.other	Epithelial Cells	en
dc.subject.other	Humans	en
dc.subject.other	Hydrogen Peroxide	en
dc.subject.other	Intestinal Mucosa	en
dc.subject.other	Lactobacillus	en
dc.subject.other	Macrophages	en
dc.subject.other	Nitric Oxide	en
dc.subject.other	Probiotics	en
dc.subject.other	Reactive Oxygen Species	en
dc.subject.other	Rotavirus	en
dc.subject.other	Transmissible gastroenteritis virus	en
dc.subject.other	Animalia	en
dc.subject.other	Enterococcus faecium	en
dc.subject.other	Lactobacillus casei	en
dc.subject.other	Lactobacillus fermentum	en
dc.subject.other	Lactobacillus pentosus	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	Lactobacillus rhamnosus	en
dc.subject.other	Rotavirus	en
dc.subject.other	Transmissible gastroenteritis virus	en
dc.title	Lactic acid bacteria efficiently protect human and animal intestinal epithelial and immune cells from enteric virus infection	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijfoodmicro.2009.12.024	en
heal.publicationDate	2010	en
heal.abstract	This study aimed to examine the potential antiviral activity of lactic acid bacteria (LAB) using animal and human intestinal and macrophage cell line models of non tumor origin. To this end, LAB strains selected on the basis of previous in vitro trials were co-incubated with cell line monolayers, which were subsequently challenged with rotavirus (RV) and transmissible gastroenteritis virus (TGEV). In order to elucidate the possible mechanism responsible for the antiviral activity, the induction of reactive oxygen species (ROS) release as well as the attachment ability of LAB on the cell lines was investigated. Various strains were found to exhibit moderate to complete monolayer protection against viral RV or TGEV disruption. Highest protection effects were recorded with the known probiotics Lactobacillus rhamnosus GG and Lactobacillus casei Shirota against both RV and TGEV, while notable antiviral activity was also attributed to Enterococcus faecium PCK38, Lactobacillus fermentum ACA-DC179, Lactobacillus pentosus PCA227 and Lactobacillus plantarum PCA236 and PCS22, depending on the cell line and virus combination used. A variable increase (of up to 50%) on the release of NO- and H2O2 (ROS) was obtained when LAB strains were co-incubated with the cell lines, but the results were found to be LAB strain and cell line specific, apart from a small number of strains which were able to induce strong ROS release in more than one cell line. In contrast, the ability of the examined LAB strains to attach to the cell line monolayers was LAB strain but not cell line specific. Highest attachment ability was observed with L. plantarum ACA-DC 146, L. paracasei subsp. tolerans ACA-DC 4037 and E. faecium PCD71. Clear indications on the nature of the antiviral effect were evident only in the case of the L. casei Shirota against TGEV and with L. plantarum PCA236 againt both RV and TGEV. In the rest of the cases, each interaction was LAB-cell line-virus specific, barring general conclusions. However, it is probable that more than one mechanism is involved in the antiviral effect described here. Further investigations are required to elucidate the underlying mode of action and to develop a cell line model as a system for selection of probiotic strains suited for farm animal applications. © 2010 Elsevier B.V.	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.issue	SUPPL.	en
dc.identifier.volume	141	en
dc.identifier.doi	10.1016/j.ijfoodmicro.2009.12.024	en
dc.identifier.spage	S91	en
dc.identifier.epage	S97	en