Insights into the role of ethylene perception in tomato resistance to vascular infection by Verticillium dahliae

dc.contributor.author	Pantelides, IS	en
dc.contributor.author	Tjamos, SE	en
dc.contributor.author	Paplomatas, EJ	en
dc.date.accessioned	2014-06-06T06:50:32Z
dc.date.available	2014-06-06T06:50:32Z
dc.date.issued	2010	en
dc.identifier.issn	00320862	en
dc.identifier.uri	http://dx.doi.org/10.1111/j.1365-3059.2009.02206.x	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5060
dc.subject	Disease resistance	en
dc.subject	Gene silencing	en
dc.subject	Quantitative PCR	en
dc.subject	Solanum lycopersium	en
dc.subject	Verticillium wilt	en
dc.subject.other	biomass	en
dc.subject.other	disease resistance	en
dc.subject.other	disease severity	en
dc.subject.other	ethylene	en
dc.subject.other	fruit	en
dc.subject.other	fungal disease	en
dc.subject.other	gene expression	en
dc.subject.other	growth rate	en
dc.subject.other	statistical analysis	en
dc.subject.other	vascular plant	en
dc.subject.other	wilt	en
dc.subject.other	Lycopersicon esculentum	en
dc.subject.other	Nicotiana tabacum	en
dc.subject.other	Solanum	en
dc.subject.other	Tobacco rattle virus	en
dc.subject.other	Verticillium dahliae	en
dc.title	Insights into the role of ethylene perception in tomato resistance to vascular infection by Verticillium dahliae	en
heal.type	journalArticle	en
heal.identifier.primary	10.1111/j.1365-3059.2009.02206.x	en
heal.publicationDate	2010	en
heal.abstract	A Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) system was employed to investigate the role of the tomato ethylene receptor ETR4. By comparing wilting symptoms of verticillium wilt in wild-type, ethylene-insensitive Never ripe (Nr) mutant tomato plants and ETR4-silenced plants, it was demonstrated that disease severity in the Nr and ETR4-silenced plants was statistically reduced compared to wild-type plants. Disease incidence and severity were reduced by 11 and 20%, respectively, in the Nr plants compared to the wild-type plants, at 33 days post-inoculation (d.p.i.). In the ETR4-silenced plants, disease incidence and severity were reduced by 14 and 15%, respectively, compared to the TRV-only-inoculated plants, at 37 d.p.i. Quantification of Verticillium dahliae by qPCR revealed that the reduction in symptom severity in the Nr plants was associated with significant reduction of growth of the pathogen in the vascular tissues of the Nr plants compared to that in the wild-type plants, suggesting that impaired perception of ethylene via the Never-ripe receptor results in increased disease resistance. Fungal reduction was evident at each sampling day in the Nr plants, ranging from 1·5 to 1·75 times less than that in the wild-type plants. Fungal quantification in the ETR4-silenced and TRV-only-inoculated plants showed similar levels of fungal biomass. © 2009 BSPP.	en
heal.journalName	Plant Pathology	en
dc.identifier.issue	1	en
dc.identifier.volume	59	en
dc.identifier.doi	10.1111/j.1365-3059.2009.02206.x	en
dc.identifier.spage	130	en
dc.identifier.epage	138	en