Feed supplementation of Lactobacillus plantarum PCA 236 modulates gut microbiota and milk fatty acid composition in dairy goats - a preliminary study

dc.contributor.author	Maragkoudakis, PA	en
dc.contributor.author	Mountzouris, KC	en
dc.contributor.author	Rosu, C	en
dc.contributor.author	Zoumpopoulou, G	en
dc.contributor.author	Papadimitriou, K	en
dc.contributor.author	Dalaka, E	en
dc.contributor.author	Hadjipetrou, A	en
dc.contributor.author	Theofanous, G	en
dc.contributor.author	Strozzi, GP	en
dc.contributor.author	Carlini, N	en
dc.contributor.author	Zervas, G	en
dc.contributor.author	Tsakalidou, E	en
dc.date.accessioned	2014-06-06T06:50:27Z
dc.date.available	2014-06-06T06:50:27Z
dc.date.issued	2010	en
dc.identifier.issn	01681605	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.ijfoodmicro.2010.03.007	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5038
dc.subject	Antioxidant status	en
dc.subject	Faecal microflora	en
dc.subject	Goat	en
dc.subject	Immune response	en
dc.subject	Milk fatty acids	en
dc.subject	Probiotic	en
dc.subject.other	antioxidant	en
dc.subject.other	fatty acid	en
dc.subject.other	immunoglobulin A	en
dc.subject.other	immunoglobulin G	en
dc.subject.other	immunoglobulin M	en
dc.subject.other	lactic acid	en
dc.subject.other	linoleic acid	en
dc.subject.other	linolenic acid	en
dc.subject.other	milk fat	en
dc.subject.other	pca 236	en
dc.subject.other	plasma protein	en
dc.subject.other	polyunsaturated fatty acid	en
dc.subject.other	probiotic agent	en
dc.subject.other	unclassified drug	en
dc.subject.other	anaerobe	en
dc.subject.other	article	en
dc.subject.other	bacterial survival	en
dc.subject.other	Bacteroides	en
dc.subject.other	blood sampling	en
dc.subject.other	body weight	en
dc.subject.other	breed	en
dc.subject.other	cheese	en
dc.subject.other	Clostridia	en
dc.subject.other	Clostridium	en
dc.subject.other	coliform bacterium	en
dc.subject.other	controlled study	en
dc.subject.other	culture medium	en
dc.subject.other	Enterococcus	en
dc.subject.other	feces microflora	en
dc.subject.other	feeding behavior	en
dc.subject.other	female	en
dc.subject.other	field study	en
dc.subject.other	gastrointestinal tract	en
dc.subject.other	goat	en
dc.subject.other	immunoglobulin blood level	en
dc.subject.other	intestine flora	en
dc.subject.other	lactic acid bacterium	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	mesophilic bacterium	en
dc.subject.other	milk	en
dc.subject.other	nonhuman	en
dc.subject.other	population	en
dc.subject.other	sample	en
dc.subject.other	sheep	en
dc.subject.other	Streptococcus	en
dc.subject.other	supplementation	en
dc.subject.other	Animal Feed	en
dc.subject.other	Animals	en
dc.subject.other	Bacteria	en
dc.subject.other	Cheese	en
dc.subject.other	Dairying	en
dc.subject.other	Dietary Supplements	en
dc.subject.other	Fatty Acids, Unsaturated	en
dc.subject.other	Feces	en
dc.subject.other	Female	en
dc.subject.other	Food Microbiology	en
dc.subject.other	Gastrointestinal Tract	en
dc.subject.other	Goats	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	Metagenome	en
dc.subject.other	Microbial Viability	en
dc.subject.other	Milk	en
dc.subject.other	Probiotics	en
dc.subject.other	Animalia	en
dc.subject.other	Bacteroides	en
dc.subject.other	Capra hircus	en
dc.subject.other	Clostridia	en
dc.subject.other	Clostridium	en
dc.subject.other	Enterococcus	en
dc.subject.other	Lactobacillus	en
dc.subject.other	Lactobacillus plantarum	en
dc.subject.other	Ovis aries	en
dc.subject.other	Streptococcus	en
dc.title	Feed supplementation of Lactobacillus plantarum PCA 236 modulates gut microbiota and milk fatty acid composition in dairy goats - a preliminary study	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.ijfoodmicro.2010.03.007	en
heal.publicationDate	2010	en
heal.abstract	This study aimed to evaluate the potential of a promising Lactobacillus plantarum isolate (PCA 236) from cheese as a probiotic feed supplement in lactating goats. The ability of L. plantarum to survive transit through the goat gastrointestinal tract and to modulate selected constituents of the gut microbiota composition, monitored at faecal level was assessed. In addition, L. plantarum effects on plasma immunoglobulins and antioxidant capacity of the animals as well as on the milk fatty acid composition were determined. For the purpose of the experiment a field study was designed, involving 24 dairy goats of the Damascus breed, kept in a sheep and goat dairy farm. The goats were divided in terms of body weight in two treatments of 12 goats each, namely: control (CON) without addition of L. plantarum and probiotic (PRO) treatment with in feed administration of L. plantarum so that the goats would intake 12. log. CFU/day. The experiment lasted 5 weeks and at weekly time intervals individual faecal, blood and milk samples were collected and analysed. All faecal samples were examined for the presence of L. plantarum PCA 236. In addition, the culturable population levels of mesophilic aerobes, coliforms lactic acid bacteria (LAB), Streptococcus, Enterococcus, mesophilic anaerobes, Clostridium and Bacteroides in faeces were also determined by enumeration on specific culture media. In parallel, plasma IgA, IgM and IgG and antioxidant capacity of plasma and milk were determined. No adverse effects were observed in the animals receiving the lactobacillus during the experiment. Lactobacillus plantarum PCA 236 was recovered in the faeces of all animals in the PRO treatment. In addition, PRO treatment resulted in a significant (P≤. 0.05) increase in LAB coupled with a significant decrease in faecal clostridia populations compared to the CON treatment. The antioxidant capacity and the concentrations of immunoglobulins IgA, IgM and IgG in goat plasma did not differ between the treatments. In contrast, milk fat composition in the PRO treatment had a significantly higher content of polyunsaturated fatty acids such as linoleic, a-linolenic and rumenic acids compared to CON, while there were no differences in milk antioxidant capacity. The results obtained in this study, indicate that the L. plantarum PCA 236 strain has displayed an interesting probiotic potential, in terms of beneficially modulating the goat faecal microbiota and milk fatty acid composition that needs to be further researched. © 2010 Elsevier B.V.	en
heal.journalName	International Journal of Food Microbiology	en
dc.identifier.issue	SUPPL.	en
dc.identifier.volume	141	en
dc.identifier.doi	10.1016/j.ijfoodmicro.2010.03.007	en
dc.identifier.spage	S109	en
dc.identifier.epage	S116	en