Enzymatic and mRNA transcript response of ovine 6-phosphogluconate dehydrogenase (6PGD) in respect to different weights from weaning to four months of age

dc.contributor.author	Laliotis, GP	en
dc.contributor.author	Trivizaki, S	en
dc.contributor.author	Bizelis, I	en
dc.contributor.author	Charismiadou, MA	en
dc.contributor.author	Rogdakis, E	en
dc.date.accessioned	2014-06-06T06:50:24Z
dc.date.available	2014-06-06T06:50:24Z
dc.date.issued	2010	en
dc.identifier.issn	10495398	en
dc.identifier.uri	http://dx.doi.org/10.1080/10495391003673096	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5026
dc.subject	6PGD	en
dc.subject	Adipose tissue	en
dc.subject	cDNA	en
dc.subject	Enzymatic activity	en
dc.subject	Sheep	en
dc.subject.other	6PGD	en
dc.subject.other	Adipose tissue	en
dc.subject.other	cDNA	en
dc.subject.other	Enzymatic activities	en
dc.subject.other	Sheep	en
dc.subject.other	Transcription	en
dc.subject.other	Tissue	en
dc.subject.other	messenger RNA	en
dc.subject.other	phosphogluconate dehydrogenase	en
dc.subject.other	adipocyte	en
dc.subject.other	animal	en
dc.subject.other	animal disease	en
dc.subject.other	article	en
dc.subject.other	body weight	en
dc.subject.other	chemistry	en
dc.subject.other	cytology	en
dc.subject.other	enzymology	en
dc.subject.other	female	en
dc.subject.other	gene expression regulation	en
dc.subject.other	genetics	en
dc.subject.other	male	en
dc.subject.other	metabolism	en
dc.subject.other	physiology	en
dc.subject.other	regression analysis	en
dc.subject.other	reverse transcription polymerase chain reaction	en
dc.subject.other	sheep	en
dc.subject.other	subcutaneous fat	en
dc.subject.other	Adipocytes	en
dc.subject.other	Animals	en
dc.subject.other	Body Weight	en
dc.subject.other	Female	en
dc.subject.other	Gene Expression Regulation, Enzymologic	en
dc.subject.other	Least-Squares Analysis	en
dc.subject.other	Male	en
dc.subject.other	Phosphogluconate Dehydrogenase	en
dc.subject.other	Reverse Transcriptase Polymerase Chain Reaction	en
dc.subject.other	RNA, Messenger	en
dc.subject.other	Sheep	en
dc.subject.other	Subcutaneous Fat	en
dc.subject.other	Ovis	en
dc.subject.other	Ovis aries	en
dc.title	Enzymatic and mRNA transcript response of ovine 6-phosphogluconate dehydrogenase (6PGD) in respect to different weights from weaning to four months of age	en
heal.type	journalArticle	en
heal.identifier.primary	10.1080/10495391003673096	en
heal.publicationDate	2010	en
heal.abstract	Ovine 6-phosphogluconate dehydrogenase (6PGD), an enzyme of the pentose phosphate pathway, provides the necessary compounds of NADPH for the synthesis of fatty acids. Much of research has been conducted not only on the enzymatic level, but also on molecular level elucidating its cDNA sequence. Herein, we tried to elucidate if any correlation between enzymatic activity and expression of ovine 6PGD gene exists, in respect to two different weights from weaning to 4 months old. 18 male and 16 female lambs of Chios breed were randomly selected after weaning and assigned to two groups based on sex in a different experimental open-plan shed. Two subgroups were defined in each sex and they were slaughtered at 25kg and 30kg, respectively. Samples of adipose tissue (tail, perirenal and shoulder site) were collected and 6PGD enzymatic activity, gene expression, and characteristics of adipocytes were determined. According to the determined data, tail subcutaneous adipose tissue matures later than the others examined tissues and has a diminished lipogenic activity. A 6PGD gene expression pattern was not followed by analogous changes of its enzymatic activity, suggesting that other mechanisms such as post transcription or/and post translation regulation may be involved. Copyright © Taylor & Francis Group, LLC.	en
heal.journalName	Animal Biotechnology	en
dc.identifier.issue	3	en
dc.identifier.volume	21	en
dc.identifier.doi	10.1080/10495391003673096	en
dc.identifier.spage	149	en
dc.identifier.epage	155	en