Effect of biodiesel-derived waste glycerol impurities on biomass and 1,3-propanediol production of Clostridium butyricum VPI 1718

dc.contributor.author	Chatzifragkou, A	en
dc.contributor.author	Dietz, D	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Zeng, A-P	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:50:14Z
dc.date.available	2014-06-06T06:50:14Z
dc.date.issued	2010	en
dc.identifier.issn	00063592	en
dc.identifier.uri	http://dx.doi.org/10.1002/bit.22767	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/5001
dc.subject	1,3-propanediol	en
dc.subject	Biodiesel	en
dc.subject	Clostridium butyricum	en
dc.subject	Raw glycerol	en
dc.subject.other	Batch bioreactors	en
dc.subject.other	Biomass productions	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Continuous bioreactors	en
dc.subject.other	Double bonds	en
dc.subject.other	Fermentors	en
dc.subject.other	Growth behavior	en
dc.subject.other	Growth medium	en
dc.subject.other	High concentration	en
dc.subject.other	Inhibitory effect	en
dc.subject.other	Microbial growth	en
dc.subject.other	Propanediols	en
dc.subject.other	Raw glycerol	en
dc.subject.other	Steady state	en
dc.subject.other	Acids	en
dc.subject.other	Bioconversion	en
dc.subject.other	Biodiesel	en
dc.subject.other	Bioreactors	en
dc.subject.other	Clostridium	en
dc.subject.other	Concentration (process)	en
dc.subject.other	Methanol	en
dc.subject.other	Oleic acid	en
dc.subject.other	Sodium chloride	en
dc.subject.other	Stearic acid	en
dc.subject.other	Glycerol	en
dc.subject.other	1,3 propanediol	en
dc.subject.other	biodiesel	en
dc.subject.other	glycerol	en
dc.subject.other	methanol	en
dc.subject.other	oleic acid	en
dc.subject.other	sodium chloride	en
dc.subject.other	stearic acid	en
dc.subject.other	article	en
dc.subject.other	bacterial growth	en
dc.subject.other	batch reactor	en
dc.subject.other	biomass	en
dc.subject.other	biotransformation	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	culture medium	en
dc.subject.other	fermentation	en
dc.subject.other	nonhuman	en
dc.subject.other	Biofuels	en
dc.subject.other	Cell Proliferation	en
dc.subject.other	Clostridium butyricum	en
dc.subject.other	Dose-Response Relationship, Drug	en
dc.subject.other	Glycerol	en
dc.subject.other	Industrial Waste	en
dc.subject.other	Propylene Glycols	en
dc.subject.other	Clostridium butyricum	en
dc.title	Effect of biodiesel-derived waste glycerol impurities on biomass and 1,3-propanediol production of Clostridium butyricum VPI 1718	en
heal.type	journalArticle	en
heal.identifier.primary	10.1002/bit.22767	en
heal.publicationDate	2010	en
heal.abstract	Aim of the present study was to assess and evaluate the impact of various kinds of impurities of biodiesel-derived raw glycerol feedstock, upon microbial growth and 1,3-propanediol (1,3-PDO) production by Clostridium butyricum. Preliminary trials in 200-mL anaerobic bottles revealed that the presence of NaCl at a concentration of 4.5% (w/w of glycerol) in growth medium imposed an evident inhibitory effect, in contrast with phosphoric salts. However, the application of NaCl at elevated quantities during batch bioreactor experiments [up to 30% (w/w of glycerol)], did neither affect the microbial growth, nor the 1,3-PDO production. Moreover, when oleic acid was added into the growth medium at 2% (w/w of glycerol), a total preclusion of the strain was observed. In order to further investigate whether the nature of oleic acid itself or the presence of the double bond induced the inhibitory phenomenon, stearic acid was added into the medium at the same concentration (2%, w/w, of glycerol). Indeed, no inhibitory effect was observed in the fermentor, suggesting that the presence of the double bond may play a key role in the growth behavior of the microorganism. Finally, methanol effect was tested in batch and continuous bioreactor operations. Interestingly enough, the alcohol addition did not affect the microbial bioconversion of glycerol into 1,3-PDO, even when imposed at relatively high concentrations (10%, w/w, of glycerol) in batch-bioreactor operations. In continuous experiments, methanol was added when steady state had been achieved, and although in one case high concentration was added into the chemostat (5 g/L), the system re-obtained a steady state without indications of negative effect upon biomass production due to the alcohol. © 2010 Wiley Periodicals, Inc.	en
heal.journalName	Biotechnology and Bioengineering	en
dc.identifier.issue	1	en
dc.identifier.volume	107	en
dc.identifier.doi	10.1002/bit.22767	en
dc.identifier.spage	76	en
dc.identifier.epage	84	en