Development of a fully automated flow injection analyzer implementing bioluminescent biosensors for water toxicity assessment

dc.contributor.advisor	Georgiou, Constantinos	en
dc.contributor.advisor	Γεωργίου, Κωνσταντίνος	el
dc.contributor.author	Komaitis, Efstratios	en
dc.contributor.author	Vasiliou, Efstathios	en
dc.contributor.author	Kremmydas, Gerasimos	en
dc.contributor.author	Georgakopoulos, Dimitrios G.	en
dc.contributor.author	Κωμαΐτης, Ευστράτιος	el
dc.contributor.author	Βασιλείου, Ευστάθιος	el
dc.contributor.author	Κρεμμύδας, Γεράσιμος	el
dc.contributor.author	Γεωργακόπουλος, Δημήτριος Γ.	el
dc.date.accessioned	2014-06-06T06:50:11Z
dc.date.available	2014-06-06T06:50:11Z
dc.date.issued	2010-07-27	en
dc.identifier.issn	14248220	en
dc.identifier.uri	http://dx.doi.org/10.3390/s100807089	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4988
dc.rights	CC0 1.0 Παγκόσμια
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/
dc.title	Development of a fully automated flow injection analyzer implementing bioluminescent biosensors for water toxicity assessment	en
heal.type	journalArticle	en
heal.keyword	Flow injection	en
heal.keyword	Toxicity	en
heal.keyword	Vibrio fischeri	en
heal.keyword	Automated fluidics	en
heal.keyword	Bioluminescent biosensor	en
heal.keyword	Heavy metals	en
heal.keyword	Water toxicity	en
heal.keyword	Deionized water	en
heal.keyword	Phosphorescence	en
heal.keyword	pH	en
heal.identifier.primary	10.3390/s100807089	en
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Γενικό Τμήμα	el
heal.publicationDate	2010-07-27	en
heal.bibliographicCitation	Georgiou, Constantinos. Development of a fully automated flow injection analyzer implementing bioluminescent biosensors for water toxicity assessment. Sensors, vol. 10 (8), pp. 7089-7098, MDPI 2010	en
heal.abstract	This paper describes the development of an automated Flow Injection analyzer for water toxicity assessment. The analyzer is validated by assessing the toxicity of heavy metal (Pb2+, Hg2+ and Cu2+) solutions. One hundred μL of a Vibrio fischeri suspension are injected in a carrier solution containing different heavy metal concentrations. Biosensor cells are mixed with the toxic carrier solution in the mixing coil on the way to the detector. Response registered is % inhibition of biosensor bioluminescence due to heavy metal toxicity in comparison to that resulting by injecting the Vibrio fischeri suspension in deionised water. Carrier solutions of mercury showed higher toxicity than the other heavy metals, whereas all metals show concentration related levels of toxicity. The biosensor's response to carrier solutions of different pHs was tested. Vibrio fischeri's bioluminescence is promoted in the pH 5-10 range. Experiments indicate that the whole cell biosensor, as applied in the automated fluidic system, responds to various toxic solutions.	en
heal.publisher	Molecular Diversity Preservation International (MDPI)	en
heal.journalName	Sensors	en
dc.identifier.doi	10.3390/s100807089	en