| dc.contributor.author |
Flampouri, K |
en |
| dc.contributor.author |
Mavrikou, S |
en |
| dc.contributor.author |
Kintzios, S |
en |
| dc.contributor.author |
Miliadis, G |
en |
| dc.contributor.author |
Aplada-Sarlis, P |
en |
| dc.date.accessioned |
2014-06-06T06:50:10Z |
|
| dc.date.available |
2014-06-06T06:50:10Z |
|
| dc.date.issued |
2010 |
en |
| dc.identifier.issn |
00399140 |
en |
| dc.identifier.uri |
http://dx.doi.org/10.1016/j.talanta.2009.10.026 |
en |
| dc.identifier.uri |
http://62.217.125.90/xmlui/handle/123456789/4986 |
|
| dc.subject |
Bioelectric Recognition Assay (BERA) |
en |
| dc.subject |
Dithiocarbamates |
en |
| dc.subject |
N2a cells |
en |
| dc.subject |
Organophosphates |
en |
| dc.subject |
Tomato |
en |
| dc.subject |
Vero cells |
en |
| dc.subject.other |
Agricultural practices |
en |
| dc.subject.other |
Agricultural samples |
en |
| dc.subject.other |
Bioelectric recognition assays |
en |
| dc.subject.other |
Cell types |
en |
| dc.subject.other |
Cytosolic |
en |
| dc.subject.other |
Differential response |
en |
| dc.subject.other |
Dithiocarbamate fungicide |
en |
| dc.subject.other |
Dithiocarbamates |
en |
| dc.subject.other |
Fluorescent assays |
en |
| dc.subject.other |
Immobilized cells |
en |
| dc.subject.other |
In-cell |
en |
| dc.subject.other |
Intracellular Ca |
en |
| dc.subject.other |
N2a cells |
en |
| dc.subject.other |
Neuroblastomas |
en |
| dc.subject.other |
Organic samples |
en |
| dc.subject.other |
Organophosphate insecticide Diazinon |
en |
| dc.subject.other |
Pesticide residue |
en |
| dc.subject.other |
Proficiency tests |
en |
| dc.subject.other |
Quantitative detection |
en |
| dc.subject.other |
Reproducibilities |
en |
| dc.subject.other |
Sodium alginates |
en |
| dc.subject.other |
Trace level |
en |
| dc.subject.other |
Vero cells |
en |
| dc.subject.other |
Working principles |
en |
| dc.subject.other |
Agricultural chemicals |
en |
| dc.subject.other |
Bioelectric potentials |
en |
| dc.subject.other |
Biosensors |
en |
| dc.subject.other |
Calcium |
en |
| dc.subject.other |
Cell culture |
en |
| dc.subject.other |
Cell membranes |
en |
| dc.subject.other |
Extraction |
en |
| dc.subject.other |
Fruits |
en |
| dc.subject.other |
Sodium |
en |
| dc.subject.other |
Pesticides |
en |
| dc.subject.other |
calcium |
en |
| dc.subject.other |
dimpylate |
en |
| dc.subject.other |
drug derivative |
en |
| dc.subject.other |
insecticide |
en |
| dc.subject.other |
pesticide |
en |
| dc.subject.other |
pesticide residue |
en |
| dc.subject.other |
propineb |
en |
| dc.subject.other |
zineb |
en |
| dc.subject.other |
animal |
en |
| dc.subject.other |
article |
en |
| dc.subject.other |
cell membrane potential |
en |
| dc.subject.other |
Cercopithecus |
en |
| dc.subject.other |
chemistry |
en |
| dc.subject.other |
economics |
en |
| dc.subject.other |
fluorescence |
en |
| dc.subject.other |
genetic procedures |
en |
| dc.subject.other |
immobilized cell |
en |
| dc.subject.other |
metabolism |
en |
| dc.subject.other |
methodology |
en |
| dc.subject.other |
tomato |
en |
| dc.subject.other |
validation study |
en |
| dc.subject.other |
Vero cell |
en |
| dc.subject.other |
Animals |
en |
| dc.subject.other |
Biosensing Techniques |
en |
| dc.subject.other |
Calcium |
en |
| dc.subject.other |
Cells, Immobilized |
en |
| dc.subject.other |
Cercopithecus aethiops |
en |
| dc.subject.other |
Diazinon |
en |
| dc.subject.other |
Fluorescence |
en |
| dc.subject.other |
Insecticides |
en |
| dc.subject.other |
Lycopersicon esculentum |
en |
| dc.subject.other |
Membrane Potentials |
en |
| dc.subject.other |
Pesticide Residues |
en |
| dc.subject.other |
Pesticides |
en |
| dc.subject.other |
Vero Cells |
en |
| dc.subject.other |
Zineb |
en |
| dc.title |
Development and validation of a cellular biosensor detecting pesticide residues in tomatoes |
en |
| heal.type |
journalArticle |
en |
| heal.identifier.primary |
10.1016/j.talanta.2009.10.026 |
en |
| heal.publicationDate |
2010 |
en |
| heal.abstract |
Two of the most important categories of pesticides used in agricultural practice are organophosphates and dithiocarbamates. Their extensive and inappropriate use has rendered their reliable monitoring at trace levels more and more necessary. This study presents the construction of a rapid and sensitive cellular biosensor test based on the measurement of changes of the cell membrane potential of immobilized cells, according to the working principle of the Bioelectric Recognition Assay (BERA). The cells were immobilized by entrapment in a sodium alginate bead and directly applied in different pesticide dilutions and agricultural samples. The pesticides used were the organophosphate insecticide diazinon and the dithiocarbamate fungicide propineb. Two different cell types, N2a (neuroblastoma) and Vero (fibroblast) were used as the biosensory elements in order to investigate their differential response against the pesticides. In this way, we hoped to increase the selectivity of the assay. Based on the observed patterns of response, we demonstrate that the sensor can be used for the qualitative and, in some concentrations, quantitative detection of the pesticides with a high degree of reproducibility. The lowest detected concentration was 3 nM. Finally, for the investigation of the effects of different pesticides on the accumulation of cytosolic Ca2+, we conducted a fluorescent assay on N2a cells treated with tomato sample extracts, which were replicates of the E.U. proficiency test sample. The tomato samples were either organically grown or contained 14 different pesticides. The experimental results showed a higher increase of the intracellular Ca2+ concentration in cells treated with non-organic samples compared to the cells treated with organic samples. © 2009 Elsevier B.V. All rights reserved. |
en |
| heal.journalName |
Talanta |
en |
| dc.identifier.issue |
5 |
en |
| dc.identifier.volume |
80 |
en |
| dc.identifier.doi |
10.1016/j.talanta.2009.10.026 |
en |
| dc.identifier.spage |
1799 |
en |
| dc.identifier.epage |
1804 |
en |