dc.contributor.author | Chatzifragkou, A | en |
dc.contributor.author | Fakas, S | en |
dc.contributor.author | Galiotou-Panayotou, M | en |
dc.contributor.author | Komaitis, M | en |
dc.contributor.author | Aggelis, G | en |
dc.contributor.author | Papanikolaou, S | en |
dc.date.accessioned | 2014-06-06T06:50:06Z | |
dc.date.available | 2014-06-06T06:50:06Z | |
dc.date.issued | 2010 | en |
dc.identifier.issn | 14387697 | en |
dc.identifier.uri | http://dx.doi.org/10.1002/ejlt.201000027 | en |
dc.identifier.uri | http://62.217.125.90/xmlui/handle/123456789/4965 | |
dc.subject | γ-Linolenic acid | en |
dc.subject | Cunninghamella echinulata | en |
dc.subject | Mortierella isabellina | en |
dc.subject | Oleaginous microorganisms | en |
dc.subject | Renewable substrates | en |
dc.subject | Single cell oil | en |
dc.subject.other | Cunninghamella echinulata | en |
dc.subject.other | Fungi | en |
dc.subject.other | Mortierella | en |
dc.subject.other | Umbelopsis isabellina | en |
dc.title | Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi | en |
heal.type | journalArticle | en |
heal.identifier.primary | 10.1002/ejlt.201000027 | en |
heal.publicationDate | 2010 | en |
heal.abstract | Cunninghamella echinulata and Mortierella isabellina, fungi capable of accumulating single cell oil (SCO) containing γ-linolenic acid (GLA), were cultivated on sugar-based media, at initial substrate concentration 60g/L. It was demonstrated that the carbon source plays an essential role on lipid accumulation process. Especially, on glucose, C. echinulata produced 3.9g/L lipids containing 19.5% GLA, whereas M. isabellina achieved 9.9g/L of lipid, containing 3.5% GLA. Growth on fructose was also satisfactory for both microorganisms. Although M. isabellina failed to grow adequately on saccharose, C. echinulata presented appreciable biomass production of 17.6g/L, containing 18% of fat. Extra-cellular invertase activity was also detected in the case of C. echinulata cultures, achieving 0.5U/mL. Both molds presented satisfactory growth on molasses, with significant amounts of SCO and GLA produced, accompanied by non-negligible substrate decolorization. Scalingup in a 3-L bioreactor of M. isabellina with glucose as a substrate resulted in 17.5g/L of biomass, containing 12.7g/L of lipid. Finally, fractionation of cellular lipid for both strains showed that neutral lipids represented about 90-92% w/w upon total accumulated lipids, whilst fractions of glycolipids plus sphingolipids and phospholipids were 6-7 and 3-4%, respectively. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | en |
heal.journalName | European Journal of Lipid Science and Technology | en |
dc.identifier.issue | 9 | en |
dc.identifier.volume | 112 | en |
dc.identifier.doi | 10.1002/ejlt.201000027 | en |
dc.identifier.spage | 1048 | en |
dc.identifier.epage | 1057 | en |
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