Characterization of cultivated fungi isolated from grape marc wastes through the use of amplified rDNA restriction analysis and sequencing

dc.contributor.author	Ntougias, S	en
dc.contributor.author	Kavroulakis, N	en
dc.contributor.author	Papadopoulou, KK	en
dc.contributor.author	Ehaliotis, C	en
dc.contributor.author	Zervakis, GI	en
dc.date.accessioned	2014-06-06T06:50:05Z
dc.date.available	2014-06-06T06:50:05Z
dc.date.issued	2010	en
dc.identifier.issn	12258873	en
dc.identifier.uri	http://dx.doi.org/10.1007/s12275-010-9193-y	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4959
dc.subject	18S rRNA gene	en
dc.subject	Agricultural residues	en
dc.subject	Environmental quality	en
dc.subject	Fungal diversity	en
dc.subject	ITS	en
dc.subject	Winery by-products	en
dc.subject.other	fungal DNA	en
dc.subject.other	primer DNA	en
dc.subject.other	ribosome DNA	en
dc.subject.other	article	en
dc.subject.other	classification	en
dc.subject.other	ecosystem	en
dc.subject.other	fungal gene	en
dc.subject.other	fungus	en
dc.subject.other	genetics	en
dc.subject.other	grape	en
dc.subject.other	industrial waste	en
dc.subject.other	isolation and purification	en
dc.subject.other	microbiology	en
dc.subject.other	nucleotide sequence	en
dc.subject.other	phylogeny	en
dc.subject.other	waste disposal	en
dc.subject.other	wine	en
dc.subject.other	Base Sequence	en
dc.subject.other	DNA Primers	en
dc.subject.other	DNA, Fungal	en
dc.subject.other	DNA, Ribosomal	en
dc.subject.other	Ecosystem	en
dc.subject.other	Fungi	en
dc.subject.other	Genes, Fungal	en
dc.subject.other	Industrial Waste	en
dc.subject.other	Phylogeny	en
dc.subject.other	Refuse Disposal	en
dc.subject.other	Soil Microbiology	en
dc.subject.other	Vitis	en
dc.subject.other	Wine	en
dc.subject.other	Absidia corymbifera	en
dc.subject.other	Aspergillus	en
dc.subject.other	Aspergillus fumigatus	en
dc.subject.other	Bionectria ochroleuca	en
dc.subject.other	Fungi	en
dc.subject.other	Galactomyces geotrichum	en
dc.subject.other	Haematonectria haematococca	en
dc.subject.other	Otus	en
dc.subject.other	Penicillium	en
dc.subject.other	Trichosporon (unclassified)	en
dc.subject.other	Trichosporon mycotoxinivorans	en
dc.subject.other	Vitaceae	en
dc.title	Characterization of cultivated fungi isolated from grape marc wastes through the use of amplified rDNA restriction analysis and sequencing	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s12275-010-9193-y	en
heal.publicationDate	2010	en
heal.abstract	Microbial assessment of grape marc wastes, the residual solid by-product of the wine-industry, was performed by identifying phylogenetically the fungal culturable diversity in order to evaluate environmental and disposal safety issues and to discuss ecological considerations of applications on agricultural land. Fungal spores in grape marc were estimated to 4.7×106 per g dry weight. Fifty six fungal isolates were classified into eight operational taxonomic units (OTUs) following amplified ribosomal DNA restriction analysis (ARDRA) and colony morphology. Based on 18S rRNA gene and 5.8S rRNA gene-ITS sequencing, the isolates representing OTUs #1, #2, #3, and #4, which comprised 44.6%, 26.8%, 12.5%, and 5.3%, respectively, of the number of the total isolates, were identified as Aspergillus fumigatus, Bionectria ochroleuca, Haematonectria haematococca, and Trichosporon mycotoxinivorans. The isolates of OTU#5 demonstrated high phylogenetic affinity with Penicillium spp., while members of OTUs #6 and #7 were closer linked with Geotrichum candidum var. citri-aurantii and Mycocladus corymbifer, respectively (95.4 and 97.9% similarities in respect to their 5.8S rRNA gene-ITS sequences). The OTU#8 with a single isolate was related with Aspergillus strains. It appears that most of the fungal isolates are associated with the initial raw material. Despite the fact that some of the species identified may potentially act as pathogens, measures such as the avoidance of maintaining large and unprocessed quantities of grape marc wastes in premises without adequate aeration, together with its suitable biological treatment (e.g., composting) prior to any agriculture-related application, could eliminate any pertinent health risks. © 2010 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.	en
heal.journalName	Journal of Microbiology	en
dc.identifier.issue	3	en
dc.identifier.volume	48	en
dc.identifier.doi	10.1007/s12275-010-9193-y	en
dc.identifier.spage	297	en
dc.identifier.epage	306	en