BNYVV-derived dsRNA confers resistance to rhizomania disease of sugar beet as evidenced by a novel transgenic hairy root approach

dc.contributor.author	Pavli, OI	en
dc.contributor.author	Panopoulos, NJ	en
dc.contributor.author	Goldbach, R	en
dc.contributor.author	Skaracis, GN	en
dc.date.accessioned	2014-06-06T06:50:03Z
dc.date.available	2014-06-06T06:50:03Z
dc.date.issued	2010	en
dc.identifier.issn	09628819	en
dc.identifier.uri	http://dx.doi.org/10.1007/s11248-010-9364-y	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4952
dc.subject	Agrobacterium rhizogenes	en
dc.subject	Beet necrotic yellow vein virus	en
dc.subject	Beta vulgaris	en
dc.subject	Rhizomania	en
dc.subject	Sugar beet	en
dc.subject	Transformation	en
dc.subject.other	double stranded RNA	en
dc.subject.other	RNA directed RNA polymerase	en
dc.subject.other	small interfering RNA	en
dc.subject.other	virus protein	en
dc.subject.other	virus RNA	en
dc.subject.other	article	en
dc.subject.other	beet	en
dc.subject.other	gene vector	en
dc.subject.other	genetics	en
dc.subject.other	innate immunity	en
dc.subject.other	microbiology	en
dc.subject.other	pathogenicity	en
dc.subject.other	plant disease	en
dc.subject.other	plant root	en
dc.subject.other	plant virus	en
dc.subject.other	Plasmodiophorida	en
dc.subject.other	Rhizobium	en
dc.subject.other	RNA interference	en
dc.subject.other	RNA virus	en
dc.subject.other	seedling	en
dc.subject.other	transgenic plant	en
dc.subject.other	ultrastructure	en
dc.subject.other	virology	en
dc.subject.other	Beta vulgaris	en
dc.subject.other	Genetic Vectors	en
dc.subject.other	Immunity, Innate	en
dc.subject.other	Plant Diseases	en
dc.subject.other	Plant Roots	en
dc.subject.other	Plant Viruses	en
dc.subject.other	Plants, Genetically Modified	en
dc.subject.other	Plasmodiophorida	en
dc.subject.other	Rhizobium	en
dc.subject.other	RNA Interference	en
dc.subject.other	RNA Replicase	en
dc.subject.other	RNA Viruses	en
dc.subject.other	RNA, Double-Stranded	en
dc.subject.other	RNA, Small Interfering	en
dc.subject.other	RNA, Viral	en
dc.subject.other	Seedling	en
dc.subject.other	Viral Proteins	en
dc.subject.other	Agrobacterium rhizogenes	en
dc.subject.other	Beet necrotic yellow vein virus	en
dc.subject.other	Beta vulgaris	en
dc.subject.other	Beta vulgaris subsp. vulgaris	en
dc.title	BNYVV-derived dsRNA confers resistance to rhizomania disease of sugar beet as evidenced by a novel transgenic hairy root approach	en
heal.type	journalArticle	en
heal.identifier.primary	10.1007/s11248-010-9364-y	en
heal.publicationDate	2010	en
heal.abstract	Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in morphology to wild type roots but were characterized by a profound abundancy, rapid growth rate and, in some cases, plagiotropic development. Upon challenge inoculation, seedlings showed a considerable delay in symptom development compared to untransformed or vector-transformed seedlings, expressing dsRNA from an unrelated source. The transgenic root system of almost all seedlings contained no or very low virus titer while the non-transformed aerial parts of the same plants were found infected, leading to the conclusion that the hairy roots studied were effectively protected against the virus. This readily applicable novel method forms a plausible approach to preliminarily evaluate transgenic rhizomania resistance before proceeding in transformation and whole plant regeneration of sugar beet, a tedious and time consuming process for such a recalcitrant crop species. © 2010 The Author(s).	en
heal.journalName	Transgenic Research	en
dc.identifier.issue	5	en
dc.identifier.volume	19	en
dc.identifier.doi	10.1007/s11248-010-9364-y	en
dc.identifier.spage	915	en
dc.identifier.epage	922	en