Biotechnological conversions of bio-diesel derived waste glycerol into added-value compounds by higher fungi: production of biomass, single cell oil and oxalic acid

dc.contributor.author	Andre, A	en
dc.contributor.author	Diamantopoulou, P	en
dc.contributor.author	Philippoussis, A	en
dc.contributor.author	Sarris, D	en
dc.contributor.author	Komaitis, M	en
dc.contributor.author	Papanikolaou, S	en
dc.date.accessioned	2014-06-06T06:50:03Z
dc.date.available	2014-06-06T06:50:03Z
dc.date.issued	2010	en
dc.identifier.issn	09266690	en
dc.identifier.uri	http://dx.doi.org/10.1016/j.indcrop.2009.12.011	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4951
dc.subject	Aspergillus niger	en
dc.subject	Bio-diesel	en
dc.subject	Biomass	en
dc.subject	Crude glycerol	en
dc.subject	Lentinula edodes	en
dc.subject	Oxalic acid	en
dc.subject	Single cell oil	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Crude glycerol	en
dc.subject.other	Lentinula edodes	en
dc.subject.other	Oxalic Acid	en
dc.subject.other	Single cell oil	en
dc.subject.other	Bottles	en
dc.subject.other	Cell culture	en
dc.subject.other	Fatty acids	en
dc.subject.other	Fungi	en
dc.subject.other	Glycerol	en
dc.subject.other	Nitrogen	en
dc.subject.other	Pelletizing	en
dc.subject.other	Biomass	en
dc.subject.other	alcohol	en
dc.subject.other	bioenergy	en
dc.subject.other	biomass allocation	en
dc.subject.other	biotechnology	en
dc.subject.other	concentration (composition)	en
dc.subject.other	fungus	en
dc.subject.other	growth rate	en
dc.subject.other	microbial activity	en
dc.subject.other	nutrient cycling	en
dc.subject.other	oxalic acid	en
dc.subject.other	pH	en
dc.subject.other	production system	en
dc.subject.other	temperature effect	en
dc.subject.other	Aspergillus niger	en
dc.subject.other	Fungi	en
dc.subject.other	Lentinula edodes	en
dc.subject.other	biotechnology	en
dc.subject.other	lipid	en
dc.subject.other	synthesis	en
dc.subject.other	waste management	en
dc.title	Biotechnological conversions of bio-diesel derived waste glycerol into added-value compounds by higher fungi: production of biomass, single cell oil and oxalic acid	en
heal.type	journalArticle	en
heal.identifier.primary	10.1016/j.indcrop.2009.12.011	en
heal.publicationDate	2010	en
heal.abstract	Waste bio-diesel derived glycerol was used as the sole carbon source by higher fungi; two Lentinula edodes strains were flask cultured in carbon-limited conditions and displayed satisfactory growth in media presenting weak agitation, pH 4.0 and temperature 25 °C. Maximum biomass of 5.2 g/l was produced. Mycelia were synthesized, containing around 0.1 g of fat per g of biomass, with linoleic acid (Δ9,12C18:2) being the principal cellular fatty acid produced. Two Aspergillus niger strains were grown in nitrogen-limited flask cultures with constant nitrogen and two different initial glycerol concentrations into the medium. In 250-ml flask cultures, large-sized pellets were developed, in contrast with the trials performed in 2-l flasks. Nitrogen limitation led to oxalic acid secretion and intra-cellular lipid accumulation; in any case, sequential production of lipid and oxalic acid was observed. Initially, nitrogen limitation led to lipid accumulation. Thereafter, accumulated lipid was re-consumed and oxalic acid, in significant quantities, was secreted into the medium. In large-sized pellets, higher quantities of intra-cellular total lipid and lower quantities of oxalic acid were produced and vice versa. Maximum quantities of oxalic acid up to 20.5-21.5 g/l and lipid up to 3.1-3.5 g/l (corresponding to 0.41-0.57 g of fat per g of biomass) were produced. Lipid was mainly composed of oleic (Δ9C18:1) and linoleic (Δ9,12C18:2) acids. © 2010.	en
heal.journalName	Industrial Crops and Products	en
dc.identifier.issue	2	en
dc.identifier.volume	31	en
dc.identifier.doi	10.1016/j.indcrop.2009.12.011	en
dc.identifier.spage	407	en
dc.identifier.epage	416	en