The Anopheles gambiae Odorant Binding Protein 1 (AgamOBP1) Mediates Indole Recognition in the Antennae of Female Mosquitoes

dc.contributor.advisor	Biessmann, Harald	en
dc.contributor.author	Andronopoulou, Evi	en
dc.contributor.author	Biessmann, Max R.	en
dc.contributor.author	Douris, Vassilis	en
dc.contributor.author	Dimitratos, Spiros D.	en
dc.contributor.author	Eliopoulos, Elias	en
dc.contributor.author	Guerin, Patrick M.	en
dc.contributor.author	Iatrou, Kostas	en
dc.contributor.author	Justice, Robin W.	en
dc.contributor.author	Kröber, Thomas	en
dc.contributor.author	Marinotti, Osvaldo	en
dc.contributor.author	Tsitoura, Panagiota	en
dc.contributor.author	Woods, Daniel F.	en
dc.contributor.author	Walter, Marika F.	en
dc.contributor.author	Ανδρονοπούλου, Εύη	el
dc.contributor.author	Δουρής, Βασίλης	el
dc.contributor.author	Δημητράτος, Σπύρος Δ.	el
dc.contributor.author	Ηλιόπουλος, Ηλίας	el
dc.contributor.author	Ιατρού, Κώστας	el
dc.contributor.author	Τσίτουρα, Παναγιώτα	el
dc.date.accessioned	2014-06-06T06:49:58Z
dc.date.available	2014-06-06T06:49:58Z
dc.date.issued	2010-03-01	en
dc.identifier.uri	http://dx.doi.org/10.1371/journal.pone.0009471	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4921
dc.rights	CC0 1.0 Παγκόσμια	el
dc.rights.uri	http://creativecommons.org/publicdomain/zero/1.0/	en
dc.subject	Anopheles gambiae	en
dc.subject	Female mosquitoes	en
dc.subject	AgamOBP1	en
dc.subject	Malaria	en
dc.title	The Anopheles gambiae Odorant Binding Protein 1 (AgamOBP1) Mediates Indole Recognition in the Antennae of Female Mosquitoes	en
heal.type	journalArticle	en
heal.identifier.primary	10.1371/journal.pone.0009471	en
heal.recordProvider	University of California Irvine/Department of Molecular Biology and Biochemistry	en
heal.recordProvider	Εθνικό Κέντρο Έρευνας Φυσικών Επιστημών ΔΗΜΟΚΡΙΤΟΣ	el
heal.recordProvider	Γεωπονικό Πανεπιστήμιο Αθηνών/Επιστήμη Γεωπονικής Βιοτεχνολογίας	el
heal.publicationDate	2010-03-01	en
heal.bibliographicCitation	Biessmann, Harald. The Anopheles gambiae Odorant Binding Protein 1 (AgamOBP1) Mediates Indole Recognition in the Antennae of Female Mosquitoes, PLoS One, vol. 5 (3), pp 1-8, PLoS 2010	en
heal.abstract	Haematophagous insects are frequently carriers of parasitic diseases, including malaria. The mosquito Anopheles gambiae is the major vector of malaria in sub-Saharan Africa and is thus responsible for thousands of deaths daily. Although the role of olfaction in A. gambiae host detection has been demonstrated, little is known about the combinations of ligands and odorant binding proteins (OBPs) that can produce specific odor-related responses in vivo. We identified a ligand, indole, for an A. gambiae odorant binding protein, AgamOBP1, modeled the interaction in silico and confirmed the interaction using biochemical assays. RNAi-mediated gene silencing coupled with electrophysiological analyses confirmed that AgamOBP1 binds indole in A. gambiae and that the antennal receptor cells do not respond to indole in the absence of AgamOBP1. This case represents the first documented instance of a specific A. gambiae OBP–ligand pairing combination, demonstrates the significance of OBPs in odor recognition, and can be expanded to the identification of other ligands for OBPs of Anopheles and other medically important insects.	en
heal.publisher	Public Library of Science (PLoS)	en
heal.journalName	PLOS One	en
dc.identifier.doi	10.1371/journal.pone.0009471	en