Acetylcholinesterase point mutations in European strains of Tetranychus urticae (Acari: Tetranychidae) resistant to organophosphates

dc.contributor.author	Khajehali, J	en
dc.contributor.author	Van Leeuwen, T	en
dc.contributor.author	Grispou, M	en
dc.contributor.author	Morou, E	en
dc.contributor.author	Alout, H	en
dc.contributor.author	Weill, M	en
dc.contributor.author	Tirry, L	en
dc.contributor.author	Vontas, J	en
dc.contributor.author	Tsagkarakou, A	en
dc.date.accessioned	2014-06-06T06:49:51Z
dc.date.available	2014-06-06T06:49:51Z
dc.date.issued	2010	en
dc.identifier.issn	1526-498X	en
dc.identifier.uri	http://62.217.125.90/xmlui/handle/123456789/4838
dc.subject	Tetranychus urticae	en
dc.subject	spider mite	en
dc.subject	organophosphate resistance	en
dc.subject	acetylcholinesterase mutations	en
dc.subject	ace-1	en
dc.subject	resistance mechanisms	en
dc.subject.classification	Agronomy	en
dc.subject.classification	Entomology	en
dc.subject.other	AMINO-ACID SUBSTITUTION	en
dc.subject.other	2-SPOTTED SPIDER-MITE	en
dc.subject.other	ACE-PARALOGOUS ACETYLCHOLINESTERASE	en
dc.subject.other	CONFER INSECTICIDE RESISTANCE	en
dc.subject.other	APHIS-GOSSYPII GLOVER	en
dc.subject.other	INSENSITIVE ACETYLCHOLINESTERASE	en
dc.subject.other	CULEX-TRITAENIORHYNCHUS	en
dc.subject.other	BIOCHEMICAL-PROPERTIES	en
dc.subject.other	PLUTELLA-XYLOSTELLA	en
dc.subject.other	MOSQUITO VECTORS	en
dc.title	Acetylcholinesterase point mutations in European strains of Tetranychus urticae (Acari: Tetranychidae) resistant to organophosphates	en
heal.type	journalArticle	en
heal.language	English	en
heal.publicationDate	2010	en
heal.abstract	BACKGROUND: In Tetranychus urticae Koch, acetylcholinesterase insensitivity is often involved in organophosphate (OP) and carbamate (CARB) resistance. By combining toxicological, biochemical and molecular data from three reference laboratory and three OP selected strains (OP strains), the AChE1 mutations associated with resistance in T. urticae were characterised. RESULTS: The resistance ratios of the OP strains varied from 9 to 43 for pirimiphos-methyl, from 78 to 586 for chlorpyrifos, from 8 to 333 for methomyl and from 137 to 4164 for dimethoate. The insecticide concentration needed to inhibit 50% of the AChE1 activity was, in the OP strains, at least 2.7, 55, 58 and 31 times higher for the OP pirimiphos-methyl, chlorpyrifos oxon, paraoxon and omethoate respectively, and 87 times higher for the CARB carbaryl. By comparing the AChE1 sequence, four amino acid substitutions were detected in the OP strains: (1) F331W (Torpedo numbering) in all the three OP strains; (2) T280A found in the three OP strains but not in all clones; (3) G328A, found in two OP strains; (4) A201S found in only one OP strain. CONCLUSIONS: Four AChE1 mutations were found in resistant strains of T. urticae, and three of them, F331 W, G328A and A201S, are possibly involved in resistance to OP and CARB insecticides. Among them, F331W is probably the most important and the most common in T. urticae. It can be easily detected by the diagnostic PCR-RLFP assay developed in this study. (C) 2009 Society of Chemical Industry	en
heal.publisher	JOHN WILEY & SONS LTD	en
heal.journalName	PEST MANAGEMENT SCIENCE	en
dc.identifier.issue	2	en
dc.identifier.volume	66	en
dc.identifier.isi	ISI:000273758900016	en
dc.identifier.spage	220	en
dc.identifier.epage	228	en